Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29240 and RR42_RS29245 overlap by 4 nucleotides RR42_RS29245 and RR42_RS29250 overlap by 8 nucleotides RR42_RS29250 and RR42_RS29255 are separated by 174 nucleotides
RR42_RS29240: RR42_RS29240 - nitrile hydratase subunit alpha, at 1,914,971 to 1,915,675
_RS29240
RR42_RS29245: RR42_RS29245 - nitrile hydratase, at 1,915,672 to 1,916,343
_RS29245
RR42_RS29250: RR42_RS29250 - nitrile hydratase, at 1,916,336 to 1,916,722
_RS29250
RR42_RS29255: RR42_RS29255 - GTP cyclohydrolase, at 1,916,897 to 1,917,493
_RS29255
Position (kb)
1915
1916
1917 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1914.675 kb on + strand at 1914.675 kb on + strand at 1914.675 kb on + strand at 1914.676 kb on - strand at 1914.676 kb on - strand at 1914.676 kb on - strand at 1914.763 kb on + strand at 1914.763 kb on + strand at 1914.764 kb on - strand at 1914.764 kb on - strand at 1914.764 kb on - strand at 1914.912 kb on - strand at 1915.130 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.235 kb on + strand, within RR42_RS29240 at 1915.235 kb on + strand, within RR42_RS29240 at 1915.235 kb on + strand, within RR42_RS29240 at 1915.236 kb on - strand, within RR42_RS29240 at 1915.236 kb on - strand, within RR42_RS29240 at 1915.236 kb on - strand, within RR42_RS29240 at 1915.374 kb on - strand, within RR42_RS29240 at 1915.374 kb on - strand, within RR42_RS29240 at 1915.409 kb on + strand, within RR42_RS29240 at 1915.409 kb on + strand, within RR42_RS29240 at 1915.410 kb on - strand, within RR42_RS29240 at 1915.410 kb on - strand, within RR42_RS29240 at 1915.532 kb on + strand, within RR42_RS29240 at 1915.532 kb on + strand, within RR42_RS29240 at 1915.533 kb on - strand, within RR42_RS29240 at 1915.533 kb on - strand, within RR42_RS29240 at 1915.533 kb on - strand, within RR42_RS29240 at 1915.596 kb on + strand, within RR42_RS29240 at 1915.596 kb on + strand, within RR42_RS29240 at 1915.597 kb on - strand, within RR42_RS29240 at 1915.742 kb on + strand, within RR42_RS29245 at 1915.812 kb on + strand, within RR42_RS29245 at 1915.858 kb on + strand, within RR42_RS29245 at 1915.858 kb on + strand, within RR42_RS29245 at 1915.858 kb on + strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.873 kb on + strand, within RR42_RS29245 at 1915.876 kb on + strand, within RR42_RS29245 at 1915.877 kb on - strand, within RR42_RS29245 at 1916.131 kb on + strand, within RR42_RS29245 at 1916.131 kb on + strand, within RR42_RS29245 at 1916.132 kb on - strand, within RR42_RS29245 at 1916.230 kb on + strand at 1916.230 kb on + strand, within RR42_RS29245 at 1916.231 kb on - strand, within RR42_RS29245 at 1916.311 kb on + strand at 1916.312 kb on - strand at 1916.312 kb on - strand at 1916.312 kb on - strand at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.582 kb on + strand, within RR42_RS29250 at 1916.583 kb on - strand, within RR42_RS29250 at 1916.583 kb on - strand, within RR42_RS29250 at 1916.744 kb on + strand at 1916.825 kb on - strand at 1916.837 kb on + strand at 1916.845 kb on - strand at 1916.960 kb on - strand, within RR42_RS29255 at 1916.960 kb on - strand, within RR42_RS29255 at 1917.164 kb on + strand, within RR42_RS29255 at 1917.164 kb on + strand, within RR42_RS29255 at 1917.218 kb on + strand, within RR42_RS29255 at 1917.219 kb on - strand, within RR42_RS29255 at 1917.219 kb on - strand, within RR42_RS29255 at 1917.245 kb on - strand, within RR42_RS29255 at 1917.293 kb on + strand, within RR42_RS29255 at 1917.294 kb on - strand, within RR42_RS29255 at 1917.294 kb on - strand, within RR42_RS29255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,914,675 + +2.4 1,914,675 + +0.5 1,914,675 + +1.1 1,914,676 - +0.5 1,914,676 - +0.2 1,914,676 - -0.3 1,914,763 + -0.4 1,914,763 + -1.9 1,914,764 - -1.9 1,914,764 - -0.6 1,914,764 - +1.0 1,914,912 - +1.0 1,915,130 + RR42_RS29240 0.23 -0.8 1,915,169 + RR42_RS29240 0.28 -1.0 1,915,169 + RR42_RS29240 0.28 +1.8 1,915,169 + RR42_RS29240 0.28 -2.0 1,915,169 + RR42_RS29240 0.28 -0.0 1,915,169 + RR42_RS29240 0.28 +0.3 1,915,170 - RR42_RS29240 0.28 +1.3 1,915,170 - RR42_RS29240 0.28 -0.0 1,915,170 - RR42_RS29240 0.28 +1.1 1,915,235 + RR42_RS29240 0.37 -0.4 1,915,235 + RR42_RS29240 0.37 -0.2 1,915,235 + RR42_RS29240 0.37 -0.9 1,915,236 - RR42_RS29240 0.38 -0.7 1,915,236 - RR42_RS29240 0.38 -0.7 1,915,236 - RR42_RS29240 0.38 +1.9 1,915,374 - RR42_RS29240 0.57 -1.8 1,915,374 - RR42_RS29240 0.57 -0.7 1,915,409 + RR42_RS29240 0.62 -0.6 1,915,409 + RR42_RS29240 0.62 -0.4 1,915,410 - RR42_RS29240 0.62 +0.4 1,915,410 - RR42_RS29240 0.62 -2.5 1,915,532 + RR42_RS29240 0.80 +1.5 1,915,532 + RR42_RS29240 0.80 -0.5 1,915,533 - RR42_RS29240 0.80 -0.4 1,915,533 - RR42_RS29240 0.80 +1.1 1,915,533 - RR42_RS29240 0.80 -2.1 1,915,596 + RR42_RS29240 0.89 +0.7 1,915,596 + RR42_RS29240 0.89 +0.7 1,915,597 - RR42_RS29240 0.89 +0.6 1,915,742 + RR42_RS29245 0.10 +0.1 1,915,812 + RR42_RS29245 0.21 +0.4 1,915,858 + RR42_RS29245 0.28 +0.4 1,915,858 + RR42_RS29245 0.28 +1.9 1,915,858 + RR42_RS29245 0.28 +0.7 1,915,859 - RR42_RS29245 0.28 +0.5 1,915,859 - RR42_RS29245 0.28 -2.5 1,915,859 - RR42_RS29245 0.28 +1.3 1,915,859 - RR42_RS29245 0.28 -0.8 1,915,873 + RR42_RS29245 0.30 +0.5 1,915,876 + RR42_RS29245 0.30 +0.1 1,915,877 - RR42_RS29245 0.31 -1.9 1,916,131 + RR42_RS29245 0.68 -1.6 1,916,131 + RR42_RS29245 0.68 -0.3 1,916,132 - RR42_RS29245 0.68 +0.5 1,916,230 + +2.7 1,916,230 + RR42_RS29245 0.83 +1.0 1,916,231 - RR42_RS29245 0.83 +0.5 1,916,311 + -1.1 1,916,312 - -1.1 1,916,312 - -0.8 1,916,312 - -0.2 1,916,579 + RR42_RS29250 0.63 -0.5 1,916,579 + RR42_RS29250 0.63 +0.3 1,916,579 + RR42_RS29250 0.63 +0.4 1,916,579 + RR42_RS29250 0.63 -0.2 1,916,580 - RR42_RS29250 0.63 -0.3 1,916,580 - RR42_RS29250 0.63 +1.2 1,916,580 - RR42_RS29250 0.63 -0.3 1,916,582 + RR42_RS29250 0.64 -1.5 1,916,583 - RR42_RS29250 0.64 +0.2 1,916,583 - RR42_RS29250 0.64 -1.3 1,916,744 + -1.9 1,916,825 - +0.3 1,916,837 + -0.7 1,916,845 - -0.6 1,916,960 - RR42_RS29255 0.11 -1.1 1,916,960 - RR42_RS29255 0.11 -0.2 1,917,164 + RR42_RS29255 0.45 -1.5 1,917,164 + RR42_RS29255 0.45 -2.2 1,917,218 + RR42_RS29255 0.54 +2.0 1,917,219 - RR42_RS29255 0.54 -1.1 1,917,219 - RR42_RS29255 0.54 +2.7 1,917,245 - RR42_RS29255 0.58 -1.6 1,917,293 + RR42_RS29255 0.66 -0.3 1,917,294 - RR42_RS29255 0.66 -0.5 1,917,294 - RR42_RS29255 0.66 -1.8
Or see this region's nucleotide sequence