Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS26085

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS26075 and RR42_RS26080 are separated by 85 nucleotidesRR42_RS26080 and RR42_RS26085 are separated by 66 nucleotidesRR42_RS26085 and RR42_RS26090 are separated by 54 nucleotides RR42_RS26075: RR42_RS26075 - cytochrome D ubiquinol oxidase subunit I, at 1,199,317 to 1,200,627 _RS26075 RR42_RS26080: RR42_RS26080 - hypothetical protein, at 1,200,713 to 1,201,372 _RS26080 RR42_RS26085: RR42_RS26085 - acetoacetyl-CoA reductase, at 1,201,439 to 1,202,185 _RS26085 RR42_RS26090: RR42_RS26090 - acetyl-CoA acetyltransferase, at 1,202,240 to 1,203,415 _RS26090 Position (kb) 1201 1202 1203Strain fitness (log2 ratio) -3 -2 -1 0 1at 1200.465 kb on - strand, within RR42_RS26075at 1200.573 kb on + strandat 1200.573 kb on + strandat 1200.967 kb on + strand, within RR42_RS26080at 1201.372 kb on + strandat 1201.372 kb on + strandat 1201.372 kb on + strandat 1201.373 kb on - strandat 1201.373 kb on - strandat 1201.398 kb on - strandat 1201.398 kb on - strandat 1201.398 kb on - strandat 1201.513 kb on - strandat 1201.694 kb on - strand, within RR42_RS26085at 1201.726 kb on - strand, within RR42_RS26085at 1201.726 kb on - strand, within RR42_RS26085at 1202.025 kb on + strand, within RR42_RS26085at 1202.168 kb on + strandat 1202.169 kb on - strandat 1202.583 kb on + strand, within RR42_RS26090at 1202.584 kb on - strand, within RR42_RS26090at 1202.584 kb on - strand, within RR42_RS26090at 1202.914 kb on - strand, within RR42_RS26090at 1202.952 kb on + strand, within RR42_RS26090at 1202.952 kb on + strand, within RR42_RS26090

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,200,465 - RR42_RS26075 0.88 +0.7
1,200,573 + -1.8
1,200,573 + +0.1
1,200,967 + RR42_RS26080 0.38 -0.5
1,201,372 + -2.2
1,201,372 + +1.6
1,201,372 + -2.4
1,201,373 - +0.8
1,201,373 - -1.1
1,201,398 - -2.0
1,201,398 - -0.1
1,201,398 - -0.3
1,201,513 - -1.2
1,201,694 - RR42_RS26085 0.34 -2.8
1,201,726 - RR42_RS26085 0.38 +0.7
1,201,726 - RR42_RS26085 0.38 +0.6
1,202,025 + RR42_RS26085 0.78 +0.8
1,202,168 + -0.7
1,202,169 - +0.8
1,202,583 + RR42_RS26090 0.29 -0.3
1,202,584 - RR42_RS26090 0.29 -1.3
1,202,584 - RR42_RS26090 0.29 +0.5
1,202,914 - RR42_RS26090 0.57 +0.9
1,202,952 + RR42_RS26090 0.61 -0.9
1,202,952 + RR42_RS26090 0.61 -0.6

Or see this region's nucleotide sequence