Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23000 and RR42_RS23005 overlap by 4 nucleotides RR42_RS23005 and RR42_RS23010 are separated by 289 nucleotides RR42_RS23010 and RR42_RS23015 are separated by 163 nucleotides
RR42_RS23000: RR42_RS23000 - acylaldehyde oxidase, at 494,518 to 496,803
_RS23000
RR42_RS23005: RR42_RS23005 - hypothetical protein, at 496,800 to 497,303
_RS23005
RR42_RS23010: RR42_RS23010 - hypothetical protein, at 497,593 to 498,051
_RS23010
RR42_RS23015: RR42_RS23015 - hypothetical protein, at 498,215 to 498,757
_RS23015
Position (kb) 496
497
498 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 495.945 kb on + strand, within RR42_RS23000 at 495.945 kb on + strand, within RR42_RS23000 at 495.945 kb on + strand, within RR42_RS23000 at 495.945 kb on + strand, within RR42_RS23000 at 495.946 kb on - strand, within RR42_RS23000 at 495.946 kb on - strand, within RR42_RS23000 at 495.946 kb on - strand, within RR42_RS23000 at 495.946 kb on - strand, within RR42_RS23000 at 495.946 kb on - strand, within RR42_RS23000 at 495.946 kb on - strand, within RR42_RS23000 at 496.109 kb on + strand, within RR42_RS23000 at 496.109 kb on + strand, within RR42_RS23000 at 496.109 kb on + strand, within RR42_RS23000 at 496.110 kb on - strand, within RR42_RS23000 at 496.110 kb on - strand, within RR42_RS23000 at 496.110 kb on - strand, within RR42_RS23000 at 496.203 kb on + strand, within RR42_RS23000 at 496.204 kb on - strand, within RR42_RS23000 at 496.204 kb on - strand, within RR42_RS23000 at 496.206 kb on - strand, within RR42_RS23000 at 496.206 kb on - strand, within RR42_RS23000 at 496.606 kb on + strand at 496.606 kb on + strand at 496.606 kb on + strand at 496.606 kb on + strand at 496.606 kb on + strand at 496.606 kb on + strand at 496.606 kb on + strand at 496.606 kb on + strand at 496.607 kb on - strand at 496.607 kb on - strand at 496.607 kb on - strand at 496.607 kb on - strand at 496.607 kb on - strand at 496.607 kb on - strand at 496.682 kb on + strand at 496.682 kb on + strand at 496.682 kb on + strand at 496.892 kb on + strand, within RR42_RS23005 at 496.892 kb on + strand, within RR42_RS23005 at 496.892 kb on + strand, within RR42_RS23005 at 496.892 kb on + strand, within RR42_RS23005 at 496.893 kb on - strand, within RR42_RS23005 at 496.893 kb on - strand, within RR42_RS23005 at 496.893 kb on - strand, within RR42_RS23005 at 496.893 kb on - strand, within RR42_RS23005 at 496.894 kb on + strand, within RR42_RS23005 at 496.894 kb on + strand, within RR42_RS23005 at 496.895 kb on - strand, within RR42_RS23005 at 496.895 kb on - strand, within RR42_RS23005 at 496.895 kb on - strand, within RR42_RS23005 at 496.895 kb on - strand, within RR42_RS23005 at 496.895 kb on - strand, within RR42_RS23005 at 496.895 kb on - strand, within RR42_RS23005 at 497.009 kb on - strand, within RR42_RS23005 at 497.098 kb on + strand, within RR42_RS23005 at 497.238 kb on + strand, within RR42_RS23005 at 497.402 kb on - strand at 497.508 kb on + strand at 497.508 kb on + strand at 497.652 kb on - strand, within RR42_RS23010 at 497.652 kb on - strand, within RR42_RS23010 at 497.652 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.937 kb on - strand, within RR42_RS23010 at 498.151 kb on + strand at 498.151 kb on + strand at 498.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 495,945 + RR42_RS23000 0.62 +0.3 495,945 + RR42_RS23000 0.62 +4.9 495,945 + RR42_RS23000 0.62 +2.5 495,945 + RR42_RS23000 0.62 -0.8 495,946 - RR42_RS23000 0.62 +2.9 495,946 - RR42_RS23000 0.62 +0.6 495,946 - RR42_RS23000 0.62 +0.4 495,946 - RR42_RS23000 0.62 -0.3 495,946 - RR42_RS23000 0.62 +1.0 495,946 - RR42_RS23000 0.62 +0.4 496,109 + RR42_RS23000 0.70 -0.9 496,109 + RR42_RS23000 0.70 -0.5 496,109 + RR42_RS23000 0.70 -2.1 496,110 - RR42_RS23000 0.70 +1.3 496,110 - RR42_RS23000 0.70 +0.2 496,110 - RR42_RS23000 0.70 +1.0 496,203 + RR42_RS23000 0.74 +0.2 496,204 - RR42_RS23000 0.74 -1.0 496,204 - RR42_RS23000 0.74 -0.7 496,206 - RR42_RS23000 0.74 -1.4 496,206 - RR42_RS23000 0.74 -0.7 496,606 + -1.4 496,606 + +1.6 496,606 + +1.1 496,606 + -1.8 496,606 + -1.6 496,606 + +1.2 496,606 + +0.6 496,606 + +0.3 496,607 - +0.7 496,607 - -0.3 496,607 - -0.5 496,607 - -0.4 496,607 - +0.3 496,607 - +1.7 496,682 + -1.0 496,682 + +1.3 496,682 + -1.2 496,892 + RR42_RS23005 0.18 +0.4 496,892 + RR42_RS23005 0.18 +0.9 496,892 + RR42_RS23005 0.18 +1.4 496,892 + RR42_RS23005 0.18 +0.6 496,893 - RR42_RS23005 0.18 -0.1 496,893 - RR42_RS23005 0.18 +1.0 496,893 - RR42_RS23005 0.18 +0.2 496,893 - RR42_RS23005 0.18 -1.0 496,894 + RR42_RS23005 0.19 +0.0 496,894 + RR42_RS23005 0.19 -1.2 496,895 - RR42_RS23005 0.19 +0.8 496,895 - RR42_RS23005 0.19 -2.2 496,895 - RR42_RS23005 0.19 -2.3 496,895 - RR42_RS23005 0.19 +0.6 496,895 - RR42_RS23005 0.19 +0.1 496,895 - RR42_RS23005 0.19 -0.1 497,009 - RR42_RS23005 0.41 +0.5 497,098 + RR42_RS23005 0.59 -0.6 497,238 + RR42_RS23005 0.87 -1.2 497,402 - +0.8 497,508 + -1.3 497,508 + -0.4 497,652 - RR42_RS23010 0.13 +0.3 497,652 - RR42_RS23010 0.13 -0.3 497,652 - RR42_RS23010 0.13 -1.6 497,904 - RR42_RS23010 0.68 -0.1 497,904 - RR42_RS23010 0.68 -1.6 497,904 - RR42_RS23010 0.68 -0.3 497,904 - RR42_RS23010 0.68 +1.3 497,937 - RR42_RS23010 0.75 +0.6 498,151 + +1.2 498,151 + -0.3 498,151 + +0.3
Or see this region's nucleotide sequence