Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22900 and RR42_RS22905 are separated by 81 nucleotides RR42_RS22905 and RR42_RS22910 are separated by 14 nucleotides RR42_RS22910 and RR42_RS22915 are separated by 4 nucleotides
RR42_RS22900: RR42_RS22900 - 5-oxoprolinase, at 470,081 to 471,814
_RS22900
RR42_RS22905: RR42_RS22905 - myristoyl transferase, at 471,896 to 472,885
_RS22905
RR42_RS22910: RR42_RS22910 - nitrate ABC transporter ATPase, at 472,900 to 473,673
_RS22910
RR42_RS22915: RR42_RS22915 - ABC transporter permease, at 473,678 to 474,517
_RS22915
Position (kb) 471
472
473 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 470.939 kb on + strand, within RR42_RS22900 at 470.940 kb on - strand, within RR42_RS22900 at 470.957 kb on + strand, within RR42_RS22900 at 470.957 kb on + strand, within RR42_RS22900 at 470.958 kb on - strand, within RR42_RS22900 at 470.958 kb on - strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.222 kb on - strand, within RR42_RS22900 at 471.365 kb on + strand, within RR42_RS22900 at 471.366 kb on - strand, within RR42_RS22900 at 471.616 kb on + strand, within RR42_RS22900 at 471.725 kb on + strand at 471.725 kb on + strand at 471.725 kb on + strand at 471.816 kb on - strand at 472.001 kb on + strand, within RR42_RS22905 at 472.378 kb on + strand, within RR42_RS22905 at 472.535 kb on + strand, within RR42_RS22905 at 472.628 kb on + strand, within RR42_RS22905 at 472.629 kb on - strand, within RR42_RS22905 at 472.629 kb on - strand, within RR42_RS22905 at 472.629 kb on - strand, within RR42_RS22905 at 472.724 kb on + strand, within RR42_RS22905 at 472.725 kb on - strand, within RR42_RS22905 at 472.856 kb on + strand at 472.856 kb on + strand at 472.857 kb on - strand at 472.857 kb on - strand at 472.859 kb on + strand at 472.859 kb on + strand at 472.884 kb on - strand at 472.897 kb on + strand at 472.897 kb on + strand at 472.898 kb on - strand at 472.898 kb on - strand at 472.898 kb on - strand at 472.898 kb on - strand at 472.940 kb on - strand at 473.195 kb on + strand, within RR42_RS22910 at 473.196 kb on - strand, within RR42_RS22910 at 473.354 kb on + strand, within RR42_RS22910 at 473.355 kb on - strand, within RR42_RS22910 at 473.500 kb on + strand, within RR42_RS22910 at 473.500 kb on + strand, within RR42_RS22910 at 473.501 kb on - strand, within RR42_RS22910 at 473.501 kb on - strand, within RR42_RS22910 at 473.621 kb on - strand at 473.621 kb on - strand at 473.662 kb on + strand at 473.663 kb on - strand at 473.688 kb on + strand at 473.689 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 470,939 + RR42_RS22900 0.49 -0.5 470,940 - RR42_RS22900 0.50 -0.0 470,957 + RR42_RS22900 0.51 +2.3 470,957 + RR42_RS22900 0.51 +0.6 470,958 - RR42_RS22900 0.51 +0.5 470,958 - RR42_RS22900 0.51 +0.1 471,221 + RR42_RS22900 0.66 +0.8 471,221 + RR42_RS22900 0.66 -1.3 471,221 + RR42_RS22900 0.66 +0.8 471,221 + RR42_RS22900 0.66 +0.9 471,221 + RR42_RS22900 0.66 -1.9 471,222 - RR42_RS22900 0.66 +0.6 471,365 + RR42_RS22900 0.74 +1.5 471,366 - RR42_RS22900 0.74 +0.8 471,616 + RR42_RS22900 0.89 -1.1 471,725 + +1.6 471,725 + +0.6 471,725 + +0.7 471,816 - -1.3 472,001 + RR42_RS22905 0.11 -0.3 472,378 + RR42_RS22905 0.49 +0.3 472,535 + RR42_RS22905 0.65 -1.1 472,628 + RR42_RS22905 0.74 +0.1 472,629 - RR42_RS22905 0.74 +0.3 472,629 - RR42_RS22905 0.74 +0.8 472,629 - RR42_RS22905 0.74 +0.9 472,724 + RR42_RS22905 0.84 -1.9 472,725 - RR42_RS22905 0.84 -0.4 472,856 + +0.5 472,856 + -0.6 472,857 - -1.0 472,857 - +0.6 472,859 + -0.1 472,859 + +0.8 472,884 - +0.3 472,897 + -1.7 472,897 + -0.8 472,898 - -1.4 472,898 - -1.0 472,898 - +1.0 472,898 - -1.1 472,940 - +3.1 473,195 + RR42_RS22910 0.38 -0.1 473,196 - RR42_RS22910 0.38 -0.2 473,354 + RR42_RS22910 0.59 -0.3 473,355 - RR42_RS22910 0.59 +1.6 473,500 + RR42_RS22910 0.78 +0.6 473,500 + RR42_RS22910 0.78 +0.4 473,501 - RR42_RS22910 0.78 +0.9 473,501 - RR42_RS22910 0.78 -1.6 473,621 - -1.0 473,621 - -0.7 473,662 + -0.6 473,663 - -0.8 473,688 + +1.6 473,689 - +0.1
Or see this region's nucleotide sequence