Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22800 and RR42_RS22805 are separated by 248 nucleotides RR42_RS22805 and RR42_RS22810 are separated by 38 nucleotides
RR42_RS22800: RR42_RS22800 - DSBA oxidoreductase, at 447,473 to 449,029
_RS22800
RR42_RS22805: RR42_RS22805 - LysR family transcriptional regulator, at 449,278 to 450,153
_RS22805
RR42_RS22810: RR42_RS22810 - 3-oxoacyl-ACP reductase, at 450,192 to 450,992
_RS22810
Position (kb) 449
450
451 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 448.357 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.358 kb on - strand, within RR42_RS22800 at 448.447 kb on + strand, within RR42_RS22800 at 448.447 kb on + strand, within RR42_RS22800 at 448.448 kb on - strand, within RR42_RS22800 at 448.481 kb on - strand, within RR42_RS22800 at 448.875 kb on + strand at 449.243 kb on - strand at 449.373 kb on + strand, within RR42_RS22805 at 449.566 kb on + strand, within RR42_RS22805 at 449.713 kb on + strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.715 kb on + strand, within RR42_RS22805 at 449.715 kb on + strand, within RR42_RS22805 at 450.035 kb on - strand, within RR42_RS22805 at 450.067 kb on + strand at 450.068 kb on - strand at 450.068 kb on - strand at 450.119 kb on - strand at 450.121 kb on - strand at 450.213 kb on - strand at 450.245 kb on + strand at 450.245 kb on + strand at 450.245 kb on + strand at 450.248 kb on - strand at 450.248 kb on - strand at 450.460 kb on + strand, within RR42_RS22810 at 450.461 kb on - strand, within RR42_RS22810 at 450.560 kb on + strand, within RR42_RS22810 at 450.625 kb on + strand, within RR42_RS22810 at 450.626 kb on - strand, within RR42_RS22810 at 450.634 kb on + strand, within RR42_RS22810 at 450.635 kb on - strand, within RR42_RS22810 at 450.635 kb on - strand, within RR42_RS22810 at 450.821 kb on - strand, within RR42_RS22810 at 450.892 kb on - strand, within RR42_RS22810 at 451.112 kb on + strand at 451.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 448,357 + RR42_RS22800 0.57 +1.3 448,357 + RR42_RS22800 0.57 -1.2 448,357 + RR42_RS22800 0.57 -0.6 448,358 - RR42_RS22800 0.57 -0.0 448,447 + RR42_RS22800 0.63 +0.5 448,447 + RR42_RS22800 0.63 -0.4 448,448 - RR42_RS22800 0.63 +0.4 448,481 - RR42_RS22800 0.65 -2.0 448,875 + +0.3 449,243 - -0.3 449,373 + RR42_RS22805 0.11 +0.1 449,566 + RR42_RS22805 0.33 -0.5 449,713 + RR42_RS22805 0.50 +0.8 449,714 - RR42_RS22805 0.50 -1.4 449,714 - RR42_RS22805 0.50 +0.3 449,714 - RR42_RS22805 0.50 -0.3 449,714 - RR42_RS22805 0.50 +0.3 449,715 + RR42_RS22805 0.50 -0.9 449,715 + RR42_RS22805 0.50 -1.4 450,035 - RR42_RS22805 0.86 -0.8 450,067 + +0.6 450,068 - +0.6 450,068 - +2.6 450,119 - +0.4 450,121 - -0.6 450,213 - -2.0 450,245 + -0.6 450,245 + -1.1 450,245 + -1.1 450,248 - +1.3 450,248 - -0.4 450,460 + RR42_RS22810 0.33 -2.7 450,461 - RR42_RS22810 0.34 +0.1 450,560 + RR42_RS22810 0.46 +0.3 450,625 + RR42_RS22810 0.54 +0.1 450,626 - RR42_RS22810 0.54 -0.1 450,634 + RR42_RS22810 0.55 +0.3 450,635 - RR42_RS22810 0.55 -3.0 450,635 - RR42_RS22810 0.55 -1.4 450,821 - RR42_RS22810 0.79 -1.2 450,892 - RR42_RS22810 0.87 +0.1 451,112 + -0.8 451,112 + +0.3
Or see this region's nucleotide sequence