Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS22215

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS22210 and RR42_RS22215 are separated by 79 nucleotidesRR42_RS22215 and RR42_RS22220 overlap by 8 nucleotidesRR42_RS22220 and RR42_RS22225 overlap by 4 nucleotides RR42_RS22210: RR42_RS22210 - cytochrome O ubiquinol oxidase, at 321,194 to 322,252 _RS22210 RR42_RS22215: RR42_RS22215 - cytochrome o ubiquinol oxidase subunit I, at 322,332 to 324,335 _RS22215 RR42_RS22220: RR42_RS22220 - cytochrome o ubiquinol oxidase subunit III, at 324,328 to 324,984 _RS22220 RR42_RS22225: RR42_RS22225 - cytochrome C oxidase subunit III, at 324,981 to 325,388 _RS22225 Position (kb) 322 323 324 325Strain fitness (log2 ratio) -2 -1 0 1 2 3at 321.373 kb on - strand, within RR42_RS22210at 321.736 kb on + strand, within RR42_RS22210at 321.761 kb on + strand, within RR42_RS22210at 321.762 kb on - strand, within RR42_RS22210at 321.818 kb on + strand, within RR42_RS22210at 321.956 kb on + strand, within RR42_RS22210at 321.956 kb on + strand, within RR42_RS22210at 321.984 kb on + strand, within RR42_RS22210at 322.001 kb on + strand, within RR42_RS22210at 322.025 kb on + strand, within RR42_RS22210at 322.026 kb on - strand, within RR42_RS22210at 322.489 kb on - strandat 322.543 kb on - strand, within RR42_RS22215at 322.543 kb on - strand, within RR42_RS22215at 322.543 kb on - strand, within RR42_RS22215at 322.543 kb on - strand, within RR42_RS22215at 322.543 kb on - strand, within RR42_RS22215at 322.543 kb on - strand, within RR42_RS22215at 322.879 kb on - strand, within RR42_RS22215at 322.927 kb on - strand, within RR42_RS22215at 322.929 kb on + strand, within RR42_RS22215at 322.929 kb on + strand, within RR42_RS22215at 322.929 kb on + strand, within RR42_RS22215at 322.929 kb on + strand, within RR42_RS22215at 322.930 kb on - strand, within RR42_RS22215at 322.930 kb on - strand, within RR42_RS22215at 322.980 kb on - strand, within RR42_RS22215at 323.184 kb on + strand, within RR42_RS22215at 323.185 kb on - strand, within RR42_RS22215at 323.185 kb on - strand, within RR42_RS22215at 323.185 kb on - strand, within RR42_RS22215at 323.185 kb on - strand, within RR42_RS22215at 323.185 kb on - strand, within RR42_RS22215at 323.185 kb on - strand, within RR42_RS22215at 323.223 kb on + strand, within RR42_RS22215at 323.223 kb on + strand, within RR42_RS22215at 323.223 kb on + strand, within RR42_RS22215at 323.223 kb on + strand, within RR42_RS22215at 323.298 kb on + strand, within RR42_RS22215at 323.314 kb on - strand, within RR42_RS22215at 323.343 kb on + strand, within RR42_RS22215at 323.472 kb on + strand, within RR42_RS22215at 323.472 kb on + strand, within RR42_RS22215at 323.473 kb on - strand, within RR42_RS22215at 323.594 kb on + strand, within RR42_RS22215at 323.594 kb on + strand, within RR42_RS22215at 323.673 kb on + strand, within RR42_RS22215at 323.775 kb on + strand, within RR42_RS22215at 323.776 kb on - strand, within RR42_RS22215at 323.776 kb on - strand, within RR42_RS22215at 323.872 kb on - strand, within RR42_RS22215at 323.881 kb on + strand, within RR42_RS22215at 324.098 kb on + strand, within RR42_RS22215at 324.265 kb on - strandat 324.265 kb on - strandat 324.267 kb on + strandat 324.268 kb on - strandat 324.268 kb on - strandat 324.268 kb on - strandat 324.398 kb on + strand, within RR42_RS22220at 324.398 kb on + strand, within RR42_RS22220at 324.399 kb on - strand, within RR42_RS22220at 324.399 kb on - strand, within RR42_RS22220at 324.406 kb on + strand, within RR42_RS22220at 324.460 kb on + strand, within RR42_RS22220at 324.461 kb on - strand, within RR42_RS22220at 324.461 kb on - strand, within RR42_RS22220at 324.505 kb on + strand, within RR42_RS22220at 324.506 kb on - strand, within RR42_RS22220at 324.506 kb on - strand, within RR42_RS22220at 324.614 kb on - strand, within RR42_RS22220at 324.614 kb on - strand, within RR42_RS22220at 324.718 kb on + strand, within RR42_RS22220at 324.718 kb on + strand, within RR42_RS22220at 324.719 kb on - strand, within RR42_RS22220at 324.719 kb on - strand, within RR42_RS22220at 324.962 kb on - strandat 325.071 kb on - strand, within RR42_RS22225at 325.087 kb on - strand, within RR42_RS22225at 325.087 kb on - strand, within RR42_RS22225at 325.225 kb on + strand, within RR42_RS22225at 325.225 kb on + strand, within RR42_RS22225at 325.225 kb on + strand, within RR42_RS22225at 325.226 kb on - strand, within RR42_RS22225at 325.228 kb on - strand, within RR42_RS22225at 325.228 kb on - strand, within RR42_RS22225at 325.228 kb on - strand, within RR42_RS22225at 325.239 kb on - strand, within RR42_RS22225

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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321,373 - RR42_RS22210 0.17 -0.6
321,736 + RR42_RS22210 0.51 +0.5
321,761 + RR42_RS22210 0.54 +0.3
321,762 - RR42_RS22210 0.54 -0.2
321,818 + RR42_RS22210 0.59 -0.5
321,956 + RR42_RS22210 0.72 -1.0
321,956 + RR42_RS22210 0.72 -0.1
321,984 + RR42_RS22210 0.75 +0.6
322,001 + RR42_RS22210 0.76 +0.4
322,025 + RR42_RS22210 0.78 +0.4
322,026 - RR42_RS22210 0.79 -0.1
322,489 - -0.4
322,543 - RR42_RS22215 0.11 -0.9
322,543 - RR42_RS22215 0.11 -1.1
322,543 - RR42_RS22215 0.11 -0.7
322,543 - RR42_RS22215 0.11 +0.2
322,543 - RR42_RS22215 0.11 -0.9
322,543 - RR42_RS22215 0.11 -0.2
322,879 - RR42_RS22215 0.27 +0.5
322,927 - RR42_RS22215 0.30 +0.2
322,929 + RR42_RS22215 0.30 -0.2
322,929 + RR42_RS22215 0.30 +0.3
322,929 + RR42_RS22215 0.30 +0.7
322,929 + RR42_RS22215 0.30 -1.1
322,930 - RR42_RS22215 0.30 -1.1
322,930 - RR42_RS22215 0.30 +0.1
322,980 - RR42_RS22215 0.32 +0.3
323,184 + RR42_RS22215 0.43 -0.0
323,185 - RR42_RS22215 0.43 -0.3
323,185 - RR42_RS22215 0.43 +0.0
323,185 - RR42_RS22215 0.43 +0.8
323,185 - RR42_RS22215 0.43 +0.3
323,185 - RR42_RS22215 0.43 +0.7
323,185 - RR42_RS22215 0.43 -0.5
323,223 + RR42_RS22215 0.44 -1.0
323,223 + RR42_RS22215 0.44 +1.3
323,223 + RR42_RS22215 0.44 +1.6
323,223 + RR42_RS22215 0.44 +1.9
323,298 + RR42_RS22215 0.48 -0.7
323,314 - RR42_RS22215 0.49 +0.8
323,343 + RR42_RS22215 0.50 +2.2
323,472 + RR42_RS22215 0.57 -0.2
323,472 + RR42_RS22215 0.57 -1.2
323,473 - RR42_RS22215 0.57 +0.5
323,594 + RR42_RS22215 0.63 -0.7
323,594 + RR42_RS22215 0.63 +0.7
323,673 + RR42_RS22215 0.67 -0.4
323,775 + RR42_RS22215 0.72 -2.7
323,776 - RR42_RS22215 0.72 +1.7
323,776 - RR42_RS22215 0.72 +3.0
323,872 - RR42_RS22215 0.77 -1.2
323,881 + RR42_RS22215 0.77 +0.4
324,098 + RR42_RS22215 0.88 +0.7
324,265 - +0.3
324,265 - +1.4
324,267 + -1.2
324,268 - -0.2
324,268 - -0.6
324,268 - +2.1
324,398 + RR42_RS22220 0.11 -0.8
324,398 + RR42_RS22220 0.11 -2.6
324,399 - RR42_RS22220 0.11 +1.1
324,399 - RR42_RS22220 0.11 -0.6
324,406 + RR42_RS22220 0.12 +0.9
324,460 + RR42_RS22220 0.20 -0.7
324,461 - RR42_RS22220 0.20 -0.2
324,461 - RR42_RS22220 0.20 +0.2
324,505 + RR42_RS22220 0.27 +0.4
324,506 - RR42_RS22220 0.27 +0.0
324,506 - RR42_RS22220 0.27 +0.3
324,614 - RR42_RS22220 0.44 +1.0
324,614 - RR42_RS22220 0.44 -0.4
324,718 + RR42_RS22220 0.59 +1.9
324,718 + RR42_RS22220 0.59 +0.6
324,719 - RR42_RS22220 0.60 -0.8
324,719 - RR42_RS22220 0.60 -0.8
324,962 - -1.1
325,071 - RR42_RS22225 0.22 -0.5
325,087 - RR42_RS22225 0.26 -0.4
325,087 - RR42_RS22225 0.26 +0.8
325,225 + RR42_RS22225 0.60 -1.2
325,225 + RR42_RS22225 0.60 -2.6
325,225 + RR42_RS22225 0.60 +1.9
325,226 - RR42_RS22225 0.60 -0.8
325,228 - RR42_RS22225 0.61 -1.0
325,228 - RR42_RS22225 0.61 -2.4
325,228 - RR42_RS22225 0.61 +0.4
325,239 - RR42_RS22225 0.63 -0.6

Or see this region's nucleotide sequence