Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21730 and RR42_RS21735 overlap by 1 nucleotides RR42_RS21735 and RR42_RS21740 are separated by 131 nucleotides RR42_RS21740 and RR42_RS21745 are separated by 65 nucleotides
RR42_RS21730: RR42_RS21730 - pyruvate dehydrogenase subunit beta, at 203,953 to 204,969
_RS21730
RR42_RS21735: RR42_RS21735 - acetoin dehydrogenase, at 204,969 to 206,081
_RS21735
RR42_RS21740: RR42_RS21740 - acetoin dehydrogenase, at 206,213 to 206,989
_RS21740
RR42_RS21745: RR42_RS21745 - hypothetical protein, at 207,055 to 207,810
_RS21745
Position (kb) 204
205
206
207 Strain fitness (log2 ratio) -2
-1
0
1
2 at 204.118 kb on + strand, within RR42_RS21730 at 204.119 kb on - strand, within RR42_RS21730 at 204.169 kb on + strand, within RR42_RS21730 at 204.169 kb on + strand, within RR42_RS21730 at 204.170 kb on - strand, within RR42_RS21730 at 204.289 kb on + strand, within RR42_RS21730 at 204.290 kb on - strand, within RR42_RS21730 at 204.400 kb on - strand, within RR42_RS21730 at 204.436 kb on + strand, within RR42_RS21730 at 204.436 kb on + strand, within RR42_RS21730 at 204.436 kb on + strand, within RR42_RS21730 at 204.544 kb on + strand, within RR42_RS21730 at 204.904 kb on + strand at 204.904 kb on + strand at 204.904 kb on + strand at 204.905 kb on - strand at 204.905 kb on - strand at 205.302 kb on + strand, within RR42_RS21735 at 205.302 kb on + strand, within RR42_RS21735 at 205.302 kb on + strand, within RR42_RS21735 at 205.339 kb on - strand, within RR42_RS21735 at 205.503 kb on - strand, within RR42_RS21735 at 205.689 kb on + strand, within RR42_RS21735 at 205.689 kb on + strand, within RR42_RS21735 at 205.803 kb on + strand, within RR42_RS21735 at 205.803 kb on + strand, within RR42_RS21735 at 206.018 kb on + strand at 206.158 kb on + strand at 206.158 kb on + strand at 206.158 kb on + strand at 206.159 kb on - strand at 206.171 kb on + strand at 206.171 kb on + strand at 206.172 kb on - strand at 206.172 kb on - strand at 206.172 kb on - strand at 206.201 kb on - strand at 206.678 kb on + strand, within RR42_RS21740 at 206.678 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.787 kb on - strand, within RR42_RS21740 at 206.787 kb on - strand, within RR42_RS21740 at 206.787 kb on - strand, within RR42_RS21740 at 206.788 kb on + strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 204,118 + RR42_RS21730 0.16 -0.1 204,119 - RR42_RS21730 0.16 +1.3 204,169 + RR42_RS21730 0.21 -1.0 204,169 + RR42_RS21730 0.21 +1.2 204,170 - RR42_RS21730 0.21 -0.4 204,289 + RR42_RS21730 0.33 -1.6 204,290 - RR42_RS21730 0.33 +0.3 204,400 - RR42_RS21730 0.44 -0.1 204,436 + RR42_RS21730 0.47 +0.1 204,436 + RR42_RS21730 0.47 -0.3 204,436 + RR42_RS21730 0.47 +0.6 204,544 + RR42_RS21730 0.58 -0.8 204,904 + -0.2 204,904 + -1.8 204,904 + -0.4 204,905 - -2.3 204,905 - +1.2 205,302 + RR42_RS21735 0.30 -1.2 205,302 + RR42_RS21735 0.30 -2.1 205,302 + RR42_RS21735 0.30 -0.5 205,339 - RR42_RS21735 0.33 -2.7 205,503 - RR42_RS21735 0.48 -0.8 205,689 + RR42_RS21735 0.65 -1.3 205,689 + RR42_RS21735 0.65 -1.0 205,803 + RR42_RS21735 0.75 -1.6 205,803 + RR42_RS21735 0.75 -1.2 206,018 + -1.4 206,158 + +0.4 206,158 + -0.9 206,158 + +0.8 206,159 - +1.5 206,171 + +0.0 206,171 + -1.6 206,172 - +0.6 206,172 - -0.3 206,172 - -1.5 206,201 - +0.9 206,678 + RR42_RS21740 0.60 -0.8 206,678 + RR42_RS21740 0.60 -0.4 206,786 + RR42_RS21740 0.74 +0.5 206,786 + RR42_RS21740 0.74 +0.7 206,786 + RR42_RS21740 0.74 +0.0 206,786 + RR42_RS21740 0.74 +1.8 206,787 - RR42_RS21740 0.74 -0.9 206,787 - RR42_RS21740 0.74 -1.6 206,787 - RR42_RS21740 0.74 -1.0 206,788 + RR42_RS21740 0.74 -0.2 206,789 - RR42_RS21740 0.74 +0.7 206,789 - RR42_RS21740 0.74 -1.2 206,789 - RR42_RS21740 0.74 +0.7 206,789 - RR42_RS21740 0.74 +1.2
Or see this region's nucleotide sequence