Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21625 and RR42_RS21630 are separated by 381 nucleotides RR42_RS21630 and RR42_RS21635 are separated by 28 nucleotides RR42_RS21635 and RR42_RS21640 are separated by 29 nucleotides
RR42_RS21625: RR42_RS21625 - 6-hydroxynicotinate 3-monooxygenase, at 181,169 to 182,323
_RS21625
RR42_RS21630: RR42_RS21630 - Asp/Glu racemase, at 182,705 to 183,457
_RS21630
RR42_RS21635: RR42_RS21635 - alpha/beta hydrolase, at 183,486 to 184,328
_RS21635
RR42_RS21640: RR42_RS21640 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 184,358 to 185,458
_RS21640
Position (kb) 182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 182.044 kb on - strand, within RR42_RS21625 at 182.226 kb on + strand at 182.227 kb on - strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.975 kb on + strand, within RR42_RS21630 at 183.114 kb on - strand, within RR42_RS21630 at 183.116 kb on - strand, within RR42_RS21630 at 183.383 kb on + strand at 183.383 kb on + strand at 183.452 kb on + strand at 183.665 kb on + strand, within RR42_RS21635 at 183.666 kb on - strand, within RR42_RS21635 at 183.666 kb on - strand, within RR42_RS21635 at 183.669 kb on + strand, within RR42_RS21635 at 183.669 kb on + strand, within RR42_RS21635 at 183.669 kb on + strand, within RR42_RS21635 at 183.671 kb on + strand, within RR42_RS21635 at 183.910 kb on - strand, within RR42_RS21635 at 183.931 kb on - strand, within RR42_RS21635 at 184.365 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 182,044 - RR42_RS21625 0.76 +1.2 182,226 + +2.3 182,227 - +0.2 182,500 + +0.6 182,500 + +0.3 182,500 + +0.2 182,501 - -2.0 182,501 - -1.2 182,501 - -0.3 182,975 + RR42_RS21630 0.36 -1.5 183,114 - RR42_RS21630 0.54 +1.1 183,116 - RR42_RS21630 0.55 +0.3 183,383 + -0.1 183,383 + -3.1 183,452 + -0.2 183,665 + RR42_RS21635 0.21 +0.7 183,666 - RR42_RS21635 0.21 -0.6 183,666 - RR42_RS21635 0.21 +0.2 183,669 + RR42_RS21635 0.22 +1.2 183,669 + RR42_RS21635 0.22 +0.5 183,669 + RR42_RS21635 0.22 +0.2 183,671 + RR42_RS21635 0.22 -1.3 183,910 - RR42_RS21635 0.50 +3.1 183,931 - RR42_RS21635 0.53 +1.5 184,365 + -2.1
Or see this region's nucleotide sequence