Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21260 and RR42_RS21265 are separated by 106 nucleotides RR42_RS21265 and RR42_RS21270 are separated by 180 nucleotides RR42_RS21270 and RR42_RS21275 are separated by 69 nucleotides
RR42_RS21260: RR42_RS21260 - isovaleryl-CoA dehydrogenase, at 95,066 to 96,862
_RS21260
RR42_RS21265: RR42_RS21265 - ABC transporter substrate-binding protein, at 96,969 to 97,949
_RS21265
RR42_RS21270: RR42_RS21270 - phenylacetic acid degradation-like protein, at 98,130 to 98,585
_RS21270
RR42_RS21275: RR42_RS21275 - carnitine dehydratase, at 98,655 to 99,806
_RS21275
Position (kb) 96
97
98 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 95.969 kb on + strand, within RR42_RS21260 at 95.969 kb on + strand, within RR42_RS21260 at 96.251 kb on + strand, within RR42_RS21260 at 96.353 kb on + strand, within RR42_RS21260 at 96.353 kb on + strand, within RR42_RS21260 at 96.354 kb on - strand, within RR42_RS21260 at 96.355 kb on + strand, within RR42_RS21260 at 96.355 kb on + strand, within RR42_RS21260 at 96.356 kb on - strand, within RR42_RS21260 at 96.644 kb on + strand, within RR42_RS21260 at 96.645 kb on - strand, within RR42_RS21260 at 96.645 kb on - strand, within RR42_RS21260 at 96.868 kb on + strand at 96.868 kb on + strand at 96.952 kb on + strand at 96.953 kb on - strand at 96.953 kb on - strand at 97.194 kb on - strand, within RR42_RS21265 at 97.233 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.300 kb on - strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.525 kb on - strand, within RR42_RS21265 at 97.978 kb on + strand at 97.979 kb on - strand at 98.002 kb on + strand at 98.002 kb on + strand at 98.003 kb on - strand at 98.006 kb on - strand at 98.079 kb on + strand at 98.080 kb on - strand at 98.640 kb on + strand at 98.640 kb on + strand at 98.640 kb on + strand at 98.641 kb on - strand at 98.641 kb on - strand at 98.641 kb on - strand at 98.844 kb on + strand, within RR42_RS21275 at 98.845 kb on - strand, within RR42_RS21275 at 98.845 kb on - strand, within RR42_RS21275 at 98.944 kb on + strand, within RR42_RS21275 at 98.944 kb on + strand, within RR42_RS21275 at 98.945 kb on - strand, within RR42_RS21275 at 98.945 kb on - strand, within RR42_RS21275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 95,969 + RR42_RS21260 0.50 +0.6 95,969 + RR42_RS21260 0.50 -1.0 96,251 + RR42_RS21260 0.66 -1.1 96,353 + RR42_RS21260 0.72 -0.4 96,353 + RR42_RS21260 0.72 -3.6 96,354 - RR42_RS21260 0.72 -0.6 96,355 + RR42_RS21260 0.72 +0.2 96,355 + RR42_RS21260 0.72 +0.1 96,356 - RR42_RS21260 0.72 +1.3 96,644 + RR42_RS21260 0.88 -0.7 96,645 - RR42_RS21260 0.88 +1.2 96,645 - RR42_RS21260 0.88 +0.4 96,868 + -1.3 96,868 + +1.4 96,952 + -0.7 96,953 - -2.7 96,953 - +0.4 97,194 - RR42_RS21265 0.23 -0.7 97,233 + RR42_RS21265 0.27 -0.3 97,299 + RR42_RS21265 0.34 -1.3 97,299 + RR42_RS21265 0.34 +0.2 97,299 + RR42_RS21265 0.34 -0.6 97,300 - RR42_RS21265 0.34 +0.4 97,437 + RR42_RS21265 0.48 -1.2 97,437 + RR42_RS21265 0.48 +1.1 97,437 + RR42_RS21265 0.48 -1.1 97,437 + RR42_RS21265 0.48 -1.1 97,437 + RR42_RS21265 0.48 -0.7 97,437 + RR42_RS21265 0.48 +1.6 97,437 + RR42_RS21265 0.48 -0.5 97,437 + RR42_RS21265 0.48 +0.2 97,437 + RR42_RS21265 0.48 -1.4 97,438 - RR42_RS21265 0.48 +0.3 97,438 - RR42_RS21265 0.48 -1.3 97,438 - RR42_RS21265 0.48 +0.1 97,438 - RR42_RS21265 0.48 -0.6 97,525 - RR42_RS21265 0.57 -2.0 97,978 + -0.8 97,979 - -0.4 98,002 + +0.3 98,002 + +1.2 98,003 - -0.1 98,006 - -0.8 98,079 + -0.3 98,080 - -1.5 98,640 + -3.3 98,640 + -0.2 98,640 + +0.5 98,641 - -0.4 98,641 - +1.4 98,641 - -1.4 98,844 + RR42_RS21275 0.16 +0.8 98,845 - RR42_RS21275 0.16 +1.4 98,845 - RR42_RS21275 0.16 -1.7 98,944 + RR42_RS21275 0.25 +1.4 98,944 + RR42_RS21275 0.25 -1.3 98,945 - RR42_RS21275 0.25 +0.6 98,945 - RR42_RS21275 0.25 -0.9
Or see this region's nucleotide sequence