Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15235

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15220 and RR42_RS15225 are separated by 4 nucleotidesRR42_RS15225 and RR42_RS15230 are separated by 79 nucleotidesRR42_RS15230 and RR42_RS15235 are separated by 11 nucleotidesRR42_RS15235 and RR42_RS15240 are separated by 145 nucleotidesRR42_RS15240 and RR42_RS15245 are separated by 99 nucleotides RR42_RS15220: RR42_RS15220 - glutaminyl-tRNA synthetase, at 3,296,908 to 3,298,686 _RS15220 RR42_RS15225: RR42_RS15225 - RNA-binding protein, at 3,298,691 to 3,298,909 _RS15225 RR42_RS15230: RR42_RS15230 - membrane protein, at 3,298,989 to 3,299,372 _RS15230 RR42_RS15235: RR42_RS15235 - hypothetical protein, at 3,299,384 to 3,299,986 _RS15235 RR42_RS15240: RR42_RS15240 - membrane protein, at 3,300,132 to 3,300,443 _RS15240 RR42_RS15245: RR42_RS15245 - alpha/beta hydrolase, at 3,300,543 to 3,300,941 _RS15245 Position (kb) 3299 3300Strain fitness (log2 ratio) -2 -1 0 1 2at 3298.685 kb on - strandat 3298.685 kb on - strandat 3298.726 kb on + strand, within RR42_RS15225at 3299.265 kb on + strand, within RR42_RS15230at 3299.266 kb on - strand, within RR42_RS15230at 3299.266 kb on - strand, within RR42_RS15230at 3299.277 kb on + strand, within RR42_RS15230at 3299.277 kb on + strand, within RR42_RS15230at 3299.343 kb on - strandat 3299.343 kb on - strandat 3299.498 kb on + strand, within RR42_RS15235at 3299.498 kb on + strand, within RR42_RS15235at 3299.499 kb on - strand, within RR42_RS15235at 3299.499 kb on - strand, within RR42_RS15235at 3299.499 kb on - strand, within RR42_RS15235at 3299.922 kb on + strand, within RR42_RS15235at 3300.062 kb on - strandat 3300.252 kb on + strand, within RR42_RS15240at 3300.252 kb on + strand, within RR42_RS15240at 3300.253 kb on - strand, within RR42_RS15240at 3300.253 kb on - strand, within RR42_RS15240at 3300.253 kb on - strand, within RR42_RS15240at 3300.253 kb on - strand, within RR42_RS15240at 3300.253 kb on - strand, within RR42_RS15240at 3300.412 kb on - strandat 3300.412 kb on - strandat 3300.412 kb on - strandat 3300.414 kb on + strandat 3300.426 kb on + strandat 3300.427 kb on - strandat 3300.838 kb on + strand, within RR42_RS15245at 3300.850 kb on - strand, within RR42_RS15245at 3300.890 kb on + strand, within RR42_RS15245

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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3,298,685 - +0.6
3,298,685 - -0.2
3,298,726 + RR42_RS15225 0.16 -0.2
3,299,265 + RR42_RS15230 0.72 -0.4
3,299,266 - RR42_RS15230 0.72 -0.3
3,299,266 - RR42_RS15230 0.72 -0.1
3,299,277 + RR42_RS15230 0.75 +0.1
3,299,277 + RR42_RS15230 0.75 -1.1
3,299,343 - -0.1
3,299,343 - -1.2
3,299,498 + RR42_RS15235 0.19 +0.1
3,299,498 + RR42_RS15235 0.19 +0.6
3,299,499 - RR42_RS15235 0.19 +0.9
3,299,499 - RR42_RS15235 0.19 +2.0
3,299,499 - RR42_RS15235 0.19 +0.2
3,299,922 + RR42_RS15235 0.89 -0.3
3,300,062 - -0.8
3,300,252 + RR42_RS15240 0.38 -1.3
3,300,252 + RR42_RS15240 0.38 +0.4
3,300,253 - RR42_RS15240 0.39 +0.6
3,300,253 - RR42_RS15240 0.39 +0.2
3,300,253 - RR42_RS15240 0.39 -0.1
3,300,253 - RR42_RS15240 0.39 +0.8
3,300,253 - RR42_RS15240 0.39 +0.2
3,300,412 - +0.4
3,300,412 - -1.9
3,300,412 - +2.2
3,300,414 + -1.0
3,300,426 + -0.4
3,300,427 - +0.2
3,300,838 + RR42_RS15245 0.74 -0.6
3,300,850 - RR42_RS15245 0.77 -0.2
3,300,890 + RR42_RS15245 0.87 +0.5

Or see this region's nucleotide sequence