Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15220 and RR42_RS15225 are separated by 4 nucleotides RR42_RS15225 and RR42_RS15230 are separated by 79 nucleotides RR42_RS15230 and RR42_RS15235 are separated by 11 nucleotides RR42_RS15235 and RR42_RS15240 are separated by 145 nucleotides RR42_RS15240 and RR42_RS15245 are separated by 99 nucleotides
RR42_RS15220: RR42_RS15220 - glutaminyl-tRNA synthetase, at 3,296,908 to 3,298,686
_RS15220
RR42_RS15225: RR42_RS15225 - RNA-binding protein, at 3,298,691 to 3,298,909
_RS15225
RR42_RS15230: RR42_RS15230 - membrane protein, at 3,298,989 to 3,299,372
_RS15230
RR42_RS15235: RR42_RS15235 - hypothetical protein, at 3,299,384 to 3,299,986
_RS15235
RR42_RS15240: RR42_RS15240 - membrane protein, at 3,300,132 to 3,300,443
_RS15240
RR42_RS15245: RR42_RS15245 - alpha/beta hydrolase, at 3,300,543 to 3,300,941
_RS15245
Position (kb)
3299
3300 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3298.685 kb on - strand at 3298.685 kb on - strand at 3298.726 kb on + strand, within RR42_RS15225 at 3299.265 kb on + strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.343 kb on - strand at 3299.343 kb on - strand at 3299.498 kb on + strand, within RR42_RS15235 at 3299.498 kb on + strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.922 kb on + strand, within RR42_RS15235 at 3300.062 kb on - strand at 3300.252 kb on + strand, within RR42_RS15240 at 3300.252 kb on + strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.412 kb on - strand at 3300.412 kb on - strand at 3300.412 kb on - strand at 3300.414 kb on + strand at 3300.426 kb on + strand at 3300.427 kb on - strand at 3300.838 kb on + strand, within RR42_RS15245 at 3300.850 kb on - strand, within RR42_RS15245 at 3300.890 kb on + strand, within RR42_RS15245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 3,298,685 - +0.6 3,298,685 - -0.2 3,298,726 + RR42_RS15225 0.16 -0.2 3,299,265 + RR42_RS15230 0.72 -0.4 3,299,266 - RR42_RS15230 0.72 -0.3 3,299,266 - RR42_RS15230 0.72 -0.1 3,299,277 + RR42_RS15230 0.75 +0.1 3,299,277 + RR42_RS15230 0.75 -1.1 3,299,343 - -0.1 3,299,343 - -1.2 3,299,498 + RR42_RS15235 0.19 +0.1 3,299,498 + RR42_RS15235 0.19 +0.6 3,299,499 - RR42_RS15235 0.19 +0.9 3,299,499 - RR42_RS15235 0.19 +2.0 3,299,499 - RR42_RS15235 0.19 +0.2 3,299,922 + RR42_RS15235 0.89 -0.3 3,300,062 - -0.8 3,300,252 + RR42_RS15240 0.38 -1.3 3,300,252 + RR42_RS15240 0.38 +0.4 3,300,253 - RR42_RS15240 0.39 +0.6 3,300,253 - RR42_RS15240 0.39 +0.2 3,300,253 - RR42_RS15240 0.39 -0.1 3,300,253 - RR42_RS15240 0.39 +0.8 3,300,253 - RR42_RS15240 0.39 +0.2 3,300,412 - +0.4 3,300,412 - -1.9 3,300,412 - +2.2 3,300,414 + -1.0 3,300,426 + -0.4 3,300,427 - +0.2 3,300,838 + RR42_RS15245 0.74 -0.6 3,300,850 - RR42_RS15245 0.77 -0.2 3,300,890 + RR42_RS15245 0.87 +0.5
Or see this region's nucleotide sequence