Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14720 and RR42_RS14725 are separated by 140 nucleotides
RR42_RS14720: RR42_RS14720 - RNA helicase, at 3,171,441 to 3,173,381
_RS14720
RR42_RS14725: RR42_RS14725 - glycosyl transferase, at 3,173,522 to 3,174,781
_RS14725
Position (kb)
3171
3172
3173
3174 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3170.469 kb on + strand at 3170.496 kb on - strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.655 kb on - strand at 3170.772 kb on - strand at 3170.791 kb on + strand at 3170.791 kb on + strand at 3170.871 kb on + strand at 3170.871 kb on + strand at 3170.872 kb on - strand at 3170.873 kb on + strand at 3170.873 kb on + strand at 3170.873 kb on + strand at 3170.874 kb on - strand at 3170.915 kb on + strand at 3170.915 kb on + strand at 3170.916 kb on - strand at 3170.916 kb on - strand at 3170.916 kb on - strand at 3171.009 kb on + strand at 3171.144 kb on + strand at 3171.144 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.247 kb on + strand at 3171.247 kb on + strand at 3171.247 kb on + strand at 3171.247 kb on + strand at 3171.367 kb on + strand at 3171.399 kb on + strand at 3171.960 kb on + strand, within RR42_RS14720 at 3172.659 kb on + strand, within RR42_RS14720 at 3173.432 kb on + strand at 3173.432 kb on + strand at 3173.432 kb on + strand at 3173.433 kb on - strand at 3173.523 kb on + strand at 3173.524 kb on - strand at 3173.664 kb on + strand, within RR42_RS14725 at 3173.664 kb on + strand, within RR42_RS14725 at 3173.664 kb on + strand, within RR42_RS14725 at 3173.664 kb on + strand, within RR42_RS14725 at 3173.665 kb on - strand, within RR42_RS14725 at 3173.665 kb on - strand, within RR42_RS14725 at 3173.665 kb on - strand, within RR42_RS14725 at 3173.709 kb on + strand, within RR42_RS14725 at 3173.710 kb on - strand, within RR42_RS14725 at 3174.009 kb on + strand, within RR42_RS14725 at 3174.009 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.089 kb on - strand, within RR42_RS14725 at 3174.307 kb on - strand, within RR42_RS14725 at 3174.307 kb on - strand, within RR42_RS14725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 3,170,469 + -2.2 3,170,496 - -0.5 3,170,519 + +0.9 3,170,519 + +2.1 3,170,519 + +0.6 3,170,519 + +0.5 3,170,520 - -0.2 3,170,520 - -0.8 3,170,520 - +0.8 3,170,520 - -0.4 3,170,520 - -0.5 3,170,655 - +1.2 3,170,772 - +0.4 3,170,791 + -0.8 3,170,791 + +0.3 3,170,871 + -0.6 3,170,871 + -2.5 3,170,872 - +1.7 3,170,873 + -0.4 3,170,873 + -0.8 3,170,873 + -0.1 3,170,874 - -0.4 3,170,915 + -1.2 3,170,915 + +0.3 3,170,916 - +1.1 3,170,916 - +0.1 3,170,916 - -1.2 3,171,009 + +0.2 3,171,144 + +0.6 3,171,144 + +0.3 3,171,245 + -1.3 3,171,245 + -1.8 3,171,245 + +2.4 3,171,245 + -0.1 3,171,245 + +1.5 3,171,247 + +0.6 3,171,247 + -2.4 3,171,247 + +0.4 3,171,247 + +0.8 3,171,367 + -1.1 3,171,399 + -1.0 3,171,960 + RR42_RS14720 0.27 -1.5 3,172,659 + RR42_RS14720 0.63 -0.4 3,173,432 + +0.0 3,173,432 + -0.3 3,173,432 + +0.4 3,173,433 - -0.5 3,173,523 + +0.5 3,173,524 - -0.7 3,173,664 + RR42_RS14725 0.11 +0.5 3,173,664 + RR42_RS14725 0.11 -0.9 3,173,664 + RR42_RS14725 0.11 -1.3 3,173,664 + RR42_RS14725 0.11 +0.4 3,173,665 - RR42_RS14725 0.11 -1.2 3,173,665 - RR42_RS14725 0.11 +2.5 3,173,665 - RR42_RS14725 0.11 +0.3 3,173,709 + RR42_RS14725 0.15 -0.1 3,173,710 - RR42_RS14725 0.15 +1.2 3,174,009 + RR42_RS14725 0.39 +1.3 3,174,009 + RR42_RS14725 0.39 -0.3 3,174,084 + RR42_RS14725 0.45 -0.1 3,174,084 + RR42_RS14725 0.45 -1.9 3,174,084 + RR42_RS14725 0.45 +2.2 3,174,084 + RR42_RS14725 0.45 +0.1 3,174,084 + RR42_RS14725 0.45 -0.5 3,174,084 + RR42_RS14725 0.45 -0.5 3,174,084 + RR42_RS14725 0.45 -1.2 3,174,084 + RR42_RS14725 0.45 +1.1 3,174,084 + RR42_RS14725 0.45 -1.2 3,174,084 + RR42_RS14725 0.45 +0.0 3,174,084 + RR42_RS14725 0.45 -0.4 3,174,085 - RR42_RS14725 0.45 -0.4 3,174,085 - RR42_RS14725 0.45 +0.5 3,174,085 - RR42_RS14725 0.45 +0.0 3,174,085 - RR42_RS14725 0.45 -1.3 3,174,085 - RR42_RS14725 0.45 -1.3 3,174,085 - RR42_RS14725 0.45 -0.5 3,174,085 - RR42_RS14725 0.45 +0.4 3,174,085 - RR42_RS14725 0.45 -2.4 3,174,085 - RR42_RS14725 0.45 +1.5 3,174,085 - RR42_RS14725 0.45 -0.5 3,174,088 + RR42_RS14725 0.45 -0.7 3,174,088 + RR42_RS14725 0.45 +0.2 3,174,088 + RR42_RS14725 0.45 +0.8 3,174,088 + RR42_RS14725 0.45 -0.2 3,174,089 - RR42_RS14725 0.45 -1.7 3,174,307 - RR42_RS14725 0.62 +0.7 3,174,307 - RR42_RS14725 0.62 +1.9
Or see this region's nucleotide sequence