Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12455

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12445 and RR42_RS12450 are separated by 109 nucleotidesRR42_RS12450 and RR42_RS12455 are separated by 209 nucleotidesRR42_RS12455 and RR42_RS12460 are separated by 196 nucleotides RR42_RS12445: RR42_RS12445 - hypothetical protein, at 2,674,723 to 2,675,238 _RS12445 RR42_RS12450: RR42_RS12450 - hypothetical protein, at 2,675,348 to 2,675,716 _RS12450 RR42_RS12455: RR42_RS12455 - protein disaggregation chaperone, at 2,675,926 to 2,678,514 _RS12455 RR42_RS12460: RR42_RS12460 - oxidoreductase, at 2,678,711 to 2,679,640 _RS12460 Position (kb) 2675 2676 2677 2678 2679Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2675.210 kb on - strandat 2675.310 kb on + strandat 2675.310 kb on + strandat 2675.311 kb on - strandat 2675.321 kb on + strandat 2675.321 kb on + strandat 2675.322 kb on - strandat 2675.366 kb on + strandat 2675.492 kb on + strand, within RR42_RS12450at 2675.537 kb on + strand, within RR42_RS12450at 2675.558 kb on + strand, within RR42_RS12450at 2675.576 kb on + strand, within RR42_RS12450at 2675.642 kb on + strand, within RR42_RS12450at 2675.681 kb on + strandat 2675.682 kb on - strandat 2675.682 kb on - strandat 2675.682 kb on - strandat 2675.682 kb on - strandat 2675.687 kb on + strandat 2675.687 kb on + strandat 2675.687 kb on + strandat 2675.688 kb on - strandat 2675.688 kb on - strandat 2675.699 kb on + strandat 2675.836 kb on - strandat 2675.846 kb on - strandat 2675.846 kb on - strandat 2675.951 kb on + strandat 2675.952 kb on - strandat 2675.952 kb on - strandat 2675.952 kb on - strandat 2675.967 kb on - strandat 2676.086 kb on + strandat 2676.198 kb on + strand, within RR42_RS12455at 2676.287 kb on - strand, within RR42_RS12455at 2676.495 kb on - strand, within RR42_RS12455at 2676.548 kb on + strand, within RR42_RS12455at 2676.794 kb on - strand, within RR42_RS12455at 2676.812 kb on + strand, within RR42_RS12455at 2677.154 kb on + strand, within RR42_RS12455at 2677.155 kb on - strand, within RR42_RS12455at 2677.378 kb on - strand, within RR42_RS12455at 2677.379 kb on + strand, within RR42_RS12455at 2677.379 kb on + strand, within RR42_RS12455at 2677.379 kb on + strand, within RR42_RS12455at 2677.380 kb on - strand, within RR42_RS12455at 2677.448 kb on + strand, within RR42_RS12455at 2677.449 kb on - strand, within RR42_RS12455at 2677.449 kb on - strand, within RR42_RS12455at 2677.553 kb on + strand, within RR42_RS12455at 2677.690 kb on - strand, within RR42_RS12455at 2678.028 kb on - strand, within RR42_RS12455at 2678.028 kb on - strand, within RR42_RS12455at 2678.433 kb on - strandat 2678.517 kb on - strandat 2678.626 kb on - strandat 2678.685 kb on + strandat 2678.968 kb on + strand, within RR42_RS12460at 2678.969 kb on - strand, within RR42_RS12460at 2679.039 kb on + strand, within RR42_RS12460at 2679.039 kb on + strand, within RR42_RS12460at 2679.039 kb on + strand, within RR42_RS12460at 2679.040 kb on - strand, within RR42_RS12460at 2679.040 kb on - strand, within RR42_RS12460at 2679.040 kb on - strand, within RR42_RS12460at 2679.040 kb on - strand, within RR42_RS12460at 2679.073 kb on - strand, within RR42_RS12460at 2679.073 kb on - strand, within RR42_RS12460at 2679.073 kb on - strand, within RR42_RS12460at 2679.073 kb on - strand, within RR42_RS12460at 2679.073 kb on - strand, within RR42_RS12460at 2679.139 kb on - strand, within RR42_RS12460at 2679.145 kb on + strand, within RR42_RS12460at 2679.146 kb on - strand, within RR42_RS12460

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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2,675,210 - -0.3
2,675,310 + -1.5
2,675,310 + -1.6
2,675,311 - +1.2
2,675,321 + +0.4
2,675,321 + +1.1
2,675,322 - +0.5
2,675,366 + +1.5
2,675,492 + RR42_RS12450 0.39 -2.0
2,675,537 + RR42_RS12450 0.51 -1.3
2,675,558 + RR42_RS12450 0.57 +1.0
2,675,576 + RR42_RS12450 0.62 -0.0
2,675,642 + RR42_RS12450 0.80 -0.1
2,675,681 + -0.4
2,675,682 - +0.5
2,675,682 - +0.3
2,675,682 - +1.2
2,675,682 - -1.2
2,675,687 + -2.2
2,675,687 + -0.0
2,675,687 + +0.7
2,675,688 - -0.8
2,675,688 - +0.1
2,675,699 + +0.3
2,675,836 - -1.3
2,675,846 - -0.9
2,675,846 - -0.0
2,675,951 + +0.9
2,675,952 - -1.0
2,675,952 - -0.3
2,675,952 - +0.7
2,675,967 - -0.8
2,676,086 + +1.1
2,676,198 + RR42_RS12455 0.11 -1.0
2,676,287 - RR42_RS12455 0.14 -1.5
2,676,495 - RR42_RS12455 0.22 -1.4
2,676,548 + RR42_RS12455 0.24 +1.8
2,676,794 - RR42_RS12455 0.34 -0.7
2,676,812 + RR42_RS12455 0.34 -0.4
2,677,154 + RR42_RS12455 0.47 -1.0
2,677,155 - RR42_RS12455 0.47 -1.0
2,677,378 - RR42_RS12455 0.56 +1.0
2,677,379 + RR42_RS12455 0.56 -1.4
2,677,379 + RR42_RS12455 0.56 -0.0
2,677,379 + RR42_RS12455 0.56 +0.8
2,677,380 - RR42_RS12455 0.56 +0.1
2,677,448 + RR42_RS12455 0.59 +1.8
2,677,449 - RR42_RS12455 0.59 -0.3
2,677,449 - RR42_RS12455 0.59 +0.4
2,677,553 + RR42_RS12455 0.63 -0.8
2,677,690 - RR42_RS12455 0.68 +0.0
2,678,028 - RR42_RS12455 0.81 +4.1
2,678,028 - RR42_RS12455 0.81 +0.1
2,678,433 - +0.1
2,678,517 - -1.0
2,678,626 - -2.0
2,678,685 + -1.1
2,678,968 + RR42_RS12460 0.28 -1.5
2,678,969 - RR42_RS12460 0.28 +2.1
2,679,039 + RR42_RS12460 0.35 +3.1
2,679,039 + RR42_RS12460 0.35 +1.0
2,679,039 + RR42_RS12460 0.35 -1.6
2,679,040 - RR42_RS12460 0.35 -0.3
2,679,040 - RR42_RS12460 0.35 -1.4
2,679,040 - RR42_RS12460 0.35 -2.2
2,679,040 - RR42_RS12460 0.35 +0.0
2,679,073 - RR42_RS12460 0.39 -1.5
2,679,073 - RR42_RS12460 0.39 -0.2
2,679,073 - RR42_RS12460 0.39 +1.3
2,679,073 - RR42_RS12460 0.39 +0.4
2,679,073 - RR42_RS12460 0.39 +0.6
2,679,139 - RR42_RS12460 0.46 +1.0
2,679,145 + RR42_RS12460 0.47 -0.8
2,679,146 - RR42_RS12460 0.47 -0.9

Or see this region's nucleotide sequence