Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12445 and RR42_RS12450 are separated by 109 nucleotides RR42_RS12450 and RR42_RS12455 are separated by 209 nucleotides RR42_RS12455 and RR42_RS12460 are separated by 196 nucleotides
RR42_RS12445: RR42_RS12445 - hypothetical protein, at 2,674,723 to 2,675,238
_RS12445
RR42_RS12450: RR42_RS12450 - hypothetical protein, at 2,675,348 to 2,675,716
_RS12450
RR42_RS12455: RR42_RS12455 - protein disaggregation chaperone, at 2,675,926 to 2,678,514
_RS12455
RR42_RS12460: RR42_RS12460 - oxidoreductase, at 2,678,711 to 2,679,640
_RS12460
Position (kb)
2675
2676
2677
2678
2679 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2675.210 kb on - strand at 2675.310 kb on + strand at 2675.310 kb on + strand at 2675.311 kb on - strand at 2675.321 kb on + strand at 2675.321 kb on + strand at 2675.322 kb on - strand at 2675.366 kb on + strand at 2675.492 kb on + strand, within RR42_RS12450 at 2675.537 kb on + strand, within RR42_RS12450 at 2675.558 kb on + strand, within RR42_RS12450 at 2675.576 kb on + strand, within RR42_RS12450 at 2675.642 kb on + strand, within RR42_RS12450 at 2675.681 kb on + strand at 2675.682 kb on - strand at 2675.682 kb on - strand at 2675.682 kb on - strand at 2675.682 kb on - strand at 2675.687 kb on + strand at 2675.687 kb on + strand at 2675.687 kb on + strand at 2675.688 kb on - strand at 2675.688 kb on - strand at 2675.699 kb on + strand at 2675.836 kb on - strand at 2675.846 kb on - strand at 2675.846 kb on - strand at 2675.951 kb on + strand at 2675.952 kb on - strand at 2675.952 kb on - strand at 2675.952 kb on - strand at 2675.967 kb on - strand at 2676.086 kb on + strand at 2676.198 kb on + strand, within RR42_RS12455 at 2676.287 kb on - strand, within RR42_RS12455 at 2676.495 kb on - strand, within RR42_RS12455 at 2676.548 kb on + strand, within RR42_RS12455 at 2676.794 kb on - strand, within RR42_RS12455 at 2676.812 kb on + strand, within RR42_RS12455 at 2677.154 kb on + strand, within RR42_RS12455 at 2677.155 kb on - strand, within RR42_RS12455 at 2677.378 kb on - strand, within RR42_RS12455 at 2677.379 kb on + strand, within RR42_RS12455 at 2677.379 kb on + strand, within RR42_RS12455 at 2677.379 kb on + strand, within RR42_RS12455 at 2677.380 kb on - strand, within RR42_RS12455 at 2677.448 kb on + strand, within RR42_RS12455 at 2677.449 kb on - strand, within RR42_RS12455 at 2677.449 kb on - strand, within RR42_RS12455 at 2677.553 kb on + strand, within RR42_RS12455 at 2677.690 kb on - strand, within RR42_RS12455 at 2678.028 kb on - strand, within RR42_RS12455 at 2678.028 kb on - strand, within RR42_RS12455 at 2678.433 kb on - strand at 2678.517 kb on - strand at 2678.626 kb on - strand at 2678.685 kb on + strand at 2678.968 kb on + strand, within RR42_RS12460 at 2678.969 kb on - strand, within RR42_RS12460 at 2679.039 kb on + strand, within RR42_RS12460 at 2679.039 kb on + strand, within RR42_RS12460 at 2679.039 kb on + strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.139 kb on - strand, within RR42_RS12460 at 2679.145 kb on + strand, within RR42_RS12460 at 2679.146 kb on - strand, within RR42_RS12460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,675,210 - -0.3 2,675,310 + -1.5 2,675,310 + -1.6 2,675,311 - +1.2 2,675,321 + +0.4 2,675,321 + +1.1 2,675,322 - +0.5 2,675,366 + +1.5 2,675,492 + RR42_RS12450 0.39 -2.0 2,675,537 + RR42_RS12450 0.51 -1.3 2,675,558 + RR42_RS12450 0.57 +1.0 2,675,576 + RR42_RS12450 0.62 -0.0 2,675,642 + RR42_RS12450 0.80 -0.1 2,675,681 + -0.4 2,675,682 - +0.5 2,675,682 - +0.3 2,675,682 - +1.2 2,675,682 - -1.2 2,675,687 + -2.2 2,675,687 + -0.0 2,675,687 + +0.7 2,675,688 - -0.8 2,675,688 - +0.1 2,675,699 + +0.3 2,675,836 - -1.3 2,675,846 - -0.9 2,675,846 - -0.0 2,675,951 + +0.9 2,675,952 - -1.0 2,675,952 - -0.3 2,675,952 - +0.7 2,675,967 - -0.8 2,676,086 + +1.1 2,676,198 + RR42_RS12455 0.11 -1.0 2,676,287 - RR42_RS12455 0.14 -1.5 2,676,495 - RR42_RS12455 0.22 -1.4 2,676,548 + RR42_RS12455 0.24 +1.8 2,676,794 - RR42_RS12455 0.34 -0.7 2,676,812 + RR42_RS12455 0.34 -0.4 2,677,154 + RR42_RS12455 0.47 -1.0 2,677,155 - RR42_RS12455 0.47 -1.0 2,677,378 - RR42_RS12455 0.56 +1.0 2,677,379 + RR42_RS12455 0.56 -1.4 2,677,379 + RR42_RS12455 0.56 -0.0 2,677,379 + RR42_RS12455 0.56 +0.8 2,677,380 - RR42_RS12455 0.56 +0.1 2,677,448 + RR42_RS12455 0.59 +1.8 2,677,449 - RR42_RS12455 0.59 -0.3 2,677,449 - RR42_RS12455 0.59 +0.4 2,677,553 + RR42_RS12455 0.63 -0.8 2,677,690 - RR42_RS12455 0.68 +0.0 2,678,028 - RR42_RS12455 0.81 +4.1 2,678,028 - RR42_RS12455 0.81 +0.1 2,678,433 - +0.1 2,678,517 - -1.0 2,678,626 - -2.0 2,678,685 + -1.1 2,678,968 + RR42_RS12460 0.28 -1.5 2,678,969 - RR42_RS12460 0.28 +2.1 2,679,039 + RR42_RS12460 0.35 +3.1 2,679,039 + RR42_RS12460 0.35 +1.0 2,679,039 + RR42_RS12460 0.35 -1.6 2,679,040 - RR42_RS12460 0.35 -0.3 2,679,040 - RR42_RS12460 0.35 -1.4 2,679,040 - RR42_RS12460 0.35 -2.2 2,679,040 - RR42_RS12460 0.35 +0.0 2,679,073 - RR42_RS12460 0.39 -1.5 2,679,073 - RR42_RS12460 0.39 -0.2 2,679,073 - RR42_RS12460 0.39 +1.3 2,679,073 - RR42_RS12460 0.39 +0.4 2,679,073 - RR42_RS12460 0.39 +0.6 2,679,139 - RR42_RS12460 0.46 +1.0 2,679,145 + RR42_RS12460 0.47 -0.8 2,679,146 - RR42_RS12460 0.47 -0.9
Or see this region's nucleotide sequence