Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12195 and RR42_RS12200 are separated by 423 nucleotides RR42_RS12200 and RR42_RS12205 are separated by 352 nucleotides
RR42_RS12195: RR42_RS12195 - peptidase, at 2,622,490 to 2,623,158
_RS12195
RR42_RS12200: RR42_RS12200 - isocitrate lyase, at 2,623,582 to 2,624,874
_RS12200
RR42_RS12205: RR42_RS12205 - alpha/beta hydrolase, at 2,625,227 to 2,626,189
_RS12205
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.906 kb on - strand, within RR42_RS12195 at 2622.959 kb on + strand, within RR42_RS12195 at 2622.960 kb on - strand, within RR42_RS12195 at 2623.031 kb on + strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.359 kb on + strand at 2623.359 kb on + strand at 2623.388 kb on - strand at 2623.396 kb on + strand at 2623.490 kb on + strand at 2623.491 kb on - strand at 2623.686 kb on - strand at 2623.779 kb on + strand, within RR42_RS12200 at 2623.779 kb on + strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.794 kb on - strand, within RR42_RS12200 at 2623.794 kb on - strand, within RR42_RS12200 at 2623.799 kb on + strand, within RR42_RS12200 at 2623.820 kb on + strand, within RR42_RS12200 at 2623.952 kb on + strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2624.063 kb on + strand, within RR42_RS12200 at 2624.111 kb on + strand, within RR42_RS12200 at 2624.567 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.619 kb on - strand, within RR42_RS12200 at 2624.619 kb on - strand, within RR42_RS12200 at 2624.619 kb on - strand, within RR42_RS12200 at 2624.796 kb on - strand at 2624.796 kb on - strand at 2624.796 kb on - strand at 2624.892 kb on + strand at 2624.914 kb on - strand at 2625.432 kb on + strand, within RR42_RS12205 at 2625.511 kb on + strand, within RR42_RS12205 at 2625.511 kb on + strand, within RR42_RS12205 at 2625.512 kb on - strand, within RR42_RS12205 at 2625.512 kb on - strand, within RR42_RS12205 at 2625.639 kb on - strand, within RR42_RS12205 at 2625.667 kb on + strand, within RR42_RS12205 at 2625.668 kb on - strand, within RR42_RS12205 at 2625.668 kb on - strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.675 kb on + strand, within RR42_RS12205 at 2625.675 kb on + strand, within RR42_RS12205 at 2625.675 kb on + strand, within RR42_RS12205 at 2625.676 kb on - strand, within RR42_RS12205 at 2625.676 kb on - strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.789 kb on + strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,622,656 + RR42_RS12195 0.25 -1.2 2,622,656 + RR42_RS12195 0.25 -0.8 2,622,656 + RR42_RS12195 0.25 +0.8 2,622,797 + RR42_RS12195 0.46 -0.3 2,622,797 + RR42_RS12195 0.46 +0.5 2,622,905 + RR42_RS12195 0.62 +0.4 2,622,905 + RR42_RS12195 0.62 -1.0 2,622,906 - RR42_RS12195 0.62 -0.8 2,622,959 + RR42_RS12195 0.70 +0.8 2,622,960 - RR42_RS12195 0.70 +4.4 2,623,031 + RR42_RS12195 0.81 -1.1 2,623,032 - RR42_RS12195 0.81 +2.0 2,623,032 - RR42_RS12195 0.81 -1.0 2,623,359 + +1.1 2,623,359 + +0.2 2,623,388 - +0.6 2,623,396 + +0.3 2,623,490 + +0.8 2,623,491 - +1.3 2,623,686 - +1.5 2,623,779 + RR42_RS12200 0.15 -0.0 2,623,779 + RR42_RS12200 0.15 -0.6 2,623,780 - RR42_RS12200 0.15 -1.1 2,623,780 - RR42_RS12200 0.15 -2.0 2,623,780 - RR42_RS12200 0.15 +0.5 2,623,794 - RR42_RS12200 0.16 -1.1 2,623,794 - RR42_RS12200 0.16 +0.1 2,623,799 + RR42_RS12200 0.17 -0.8 2,623,820 + RR42_RS12200 0.18 -0.7 2,623,952 + RR42_RS12200 0.29 +1.6 2,623,953 - RR42_RS12200 0.29 +1.5 2,623,953 - RR42_RS12200 0.29 +1.8 2,623,953 - RR42_RS12200 0.29 -1.5 2,624,063 + RR42_RS12200 0.37 +0.8 2,624,111 + RR42_RS12200 0.41 -2.2 2,624,567 + RR42_RS12200 0.76 -1.0 2,624,618 + RR42_RS12200 0.80 -1.2 2,624,618 + RR42_RS12200 0.80 -0.2 2,624,618 + RR42_RS12200 0.80 -0.3 2,624,618 + RR42_RS12200 0.80 +0.3 2,624,618 + RR42_RS12200 0.80 -0.0 2,624,619 - RR42_RS12200 0.80 -0.8 2,624,619 - RR42_RS12200 0.80 +2.3 2,624,619 - RR42_RS12200 0.80 +1.5 2,624,796 - +0.8 2,624,796 - -1.4 2,624,796 - -1.8 2,624,892 + +0.5 2,624,914 - +0.0 2,625,432 + RR42_RS12205 0.21 -0.3 2,625,511 + RR42_RS12205 0.29 +0.3 2,625,511 + RR42_RS12205 0.29 +0.6 2,625,512 - RR42_RS12205 0.30 -1.4 2,625,512 - RR42_RS12205 0.30 -0.0 2,625,639 - RR42_RS12205 0.43 -2.7 2,625,667 + RR42_RS12205 0.46 +0.3 2,625,668 - RR42_RS12205 0.46 +1.2 2,625,668 - RR42_RS12205 0.46 +0.1 2,625,669 + RR42_RS12205 0.46 +2.1 2,625,669 + RR42_RS12205 0.46 +0.8 2,625,669 + RR42_RS12205 0.46 -2.3 2,625,669 + RR42_RS12205 0.46 -0.6 2,625,669 + RR42_RS12205 0.46 -0.1 2,625,669 + RR42_RS12205 0.46 -2.1 2,625,669 + RR42_RS12205 0.46 +0.4 2,625,670 - RR42_RS12205 0.46 +0.4 2,625,670 - RR42_RS12205 0.46 -1.4 2,625,670 - RR42_RS12205 0.46 -0.4 2,625,670 - RR42_RS12205 0.46 -1.5 2,625,670 - RR42_RS12205 0.46 -0.3 2,625,670 - RR42_RS12205 0.46 -2.0 2,625,670 - RR42_RS12205 0.46 -1.4 2,625,670 - RR42_RS12205 0.46 -2.6 2,625,675 + RR42_RS12205 0.47 +0.6 2,625,675 + RR42_RS12205 0.47 -1.9 2,625,675 + RR42_RS12205 0.47 +0.1 2,625,676 - RR42_RS12205 0.47 -1.0 2,625,676 - RR42_RS12205 0.47 +0.1 2,625,787 + RR42_RS12205 0.58 +0.1 2,625,787 + RR42_RS12205 0.58 +0.1 2,625,787 + RR42_RS12205 0.58 -0.9 2,625,788 - RR42_RS12205 0.58 -0.2 2,625,788 - RR42_RS12205 0.58 -0.5 2,625,788 - RR42_RS12205 0.58 -1.9 2,625,788 - RR42_RS12205 0.58 -1.2 2,625,788 - RR42_RS12205 0.58 -0.2 2,625,788 - RR42_RS12205 0.58 -0.7 2,625,789 + RR42_RS12205 0.58 +1.4 2,625,790 - RR42_RS12205 0.58 -0.9 2,625,790 - RR42_RS12205 0.58 -1.1 2,625,790 - RR42_RS12205 0.58 -0.0 2,625,790 - RR42_RS12205 0.58 +3.1
Or see this region's nucleotide sequence