Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12145 and RR42_RS12150 are separated by 79 nucleotides RR42_RS12150 and RR42_RS12155 are separated by 277 nucleotides
RR42_RS12145: RR42_RS12145 - adhesin, at 2,607,138 to 2,610,179
_RS12145
RR42_RS12150: RR42_RS12150 - membrane protein, at 2,610,259 to 2,611,914
_RS12150
RR42_RS12155: RR42_RS12155 - molecular chaperone, at 2,612,192 to 2,612,902
_RS12155
Position (kb)
2610
2611
2612 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2609.351 kb on + strand, within RR42_RS12145 at 2609.668 kb on + strand, within RR42_RS12145 at 2609.836 kb on - strand, within RR42_RS12145 at 2609.836 kb on - strand, within RR42_RS12145 at 2610.166 kb on + strand at 2610.166 kb on + strand at 2610.166 kb on + strand at 2610.167 kb on - strand at 2610.167 kb on - strand at 2610.170 kb on + strand at 2610.171 kb on - strand at 2610.171 kb on - strand at 2610.266 kb on + strand at 2610.568 kb on + strand, within RR42_RS12150 at 2610.647 kb on + strand, within RR42_RS12150 at 2610.647 kb on + strand, within RR42_RS12150 at 2610.785 kb on + strand, within RR42_RS12150 at 2610.785 kb on + strand, within RR42_RS12150 at 2611.046 kb on + strand, within RR42_RS12150 at 2611.053 kb on + strand, within RR42_RS12150 at 2611.054 kb on - strand, within RR42_RS12150 at 2611.194 kb on - strand, within RR42_RS12150 at 2611.226 kb on + strand, within RR42_RS12150 at 2611.238 kb on + strand, within RR42_RS12150 at 2611.238 kb on + strand, within RR42_RS12150 at 2611.238 kb on + strand, within RR42_RS12150 at 2611.238 kb on + strand, within RR42_RS12150 at 2611.239 kb on - strand, within RR42_RS12150 at 2611.396 kb on - strand, within RR42_RS12150 at 2611.580 kb on + strand, within RR42_RS12150 at 2611.580 kb on + strand, within RR42_RS12150 at 2611.682 kb on - strand, within RR42_RS12150 at 2611.874 kb on - strand at 2612.194 kb on - strand at 2612.402 kb on - strand, within RR42_RS12155 at 2612.535 kb on + strand, within RR42_RS12155 at 2612.536 kb on - strand, within RR42_RS12155 at 2612.640 kb on + strand, within RR42_RS12155 at 2612.640 kb on + strand, within RR42_RS12155
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,609,351 + RR42_RS12145 0.73 -0.6 2,609,668 + RR42_RS12145 0.83 +0.2 2,609,836 - RR42_RS12145 0.89 +0.9 2,609,836 - RR42_RS12145 0.89 -0.2 2,610,166 + +0.3 2,610,166 + +0.3 2,610,166 + +2.1 2,610,167 - -0.3 2,610,167 - +1.0 2,610,170 + -0.4 2,610,171 - +1.1 2,610,171 - -0.3 2,610,266 + +0.3 2,610,568 + RR42_RS12150 0.19 +3.1 2,610,647 + RR42_RS12150 0.23 -0.3 2,610,647 + RR42_RS12150 0.23 -0.6 2,610,785 + RR42_RS12150 0.32 -0.3 2,610,785 + RR42_RS12150 0.32 -1.1 2,611,046 + RR42_RS12150 0.48 +0.4 2,611,053 + RR42_RS12150 0.48 -3.1 2,611,054 - RR42_RS12150 0.48 -1.5 2,611,194 - RR42_RS12150 0.56 -0.2 2,611,226 + RR42_RS12150 0.58 +1.0 2,611,238 + RR42_RS12150 0.59 +0.1 2,611,238 + RR42_RS12150 0.59 -0.8 2,611,238 + RR42_RS12150 0.59 -0.6 2,611,238 + RR42_RS12150 0.59 -1.1 2,611,239 - RR42_RS12150 0.59 -1.9 2,611,396 - RR42_RS12150 0.69 -0.8 2,611,580 + RR42_RS12150 0.80 +2.0 2,611,580 + RR42_RS12150 0.80 +0.2 2,611,682 - RR42_RS12150 0.86 +0.1 2,611,874 - +0.7 2,612,194 - +2.8 2,612,402 - RR42_RS12155 0.30 +0.8 2,612,535 + RR42_RS12155 0.48 -1.5 2,612,536 - RR42_RS12155 0.48 +0.2 2,612,640 + RR42_RS12155 0.63 -0.2 2,612,640 + RR42_RS12155 0.63 +1.3
Or see this region's nucleotide sequence