Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11845 and RR42_RS11850 are separated by 11 nucleotides RR42_RS11850 and RR42_RS11855 are separated by 37 nucleotides RR42_RS11855 and RR42_RS11860 are separated by 53 nucleotides RR42_RS11860 and RR42_RS11865 are separated by 120 nucleotides
RR42_RS11845: RR42_RS11845 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 2,533,165 to 2,534,343
_RS11845
RR42_RS11850: RR42_RS11850 - phosphatidate cytidylyltransferase, at 2,534,355 to 2,535,176
_RS11850
RR42_RS11855: RR42_RS11855 - UDP diphosphate synthase, at 2,535,214 to 2,535,987
_RS11855
RR42_RS11860: RR42_RS11860 - ribosome recycling factor, at 2,536,041 to 2,536,601
_RS11860
RR42_RS11865: RR42_RS11865 - uridylate kinase, at 2,536,722 to 2,537,432
_RS11865
Position (kb)
2535
2536 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2534.363 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand at 2536.622 kb on - strand at 2536.723 kb on + strand at 2536.724 kb on - strand at 2536.726 kb on - strand at 2536.726 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,534,363 - -1.0 2,534,363 - -1.4 2,534,363 - +4.2 2,534,363 - +0.6 2,536,622 - +3.5 2,536,723 + +1.6 2,536,724 - -0.5 2,536,726 - +0.9 2,536,726 - +0.3
Or see this region's nucleotide sequence