Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11140 and RR42_RS11145 are separated by 10 nucleotides RR42_RS11145 and RR42_RS11150 are separated by 180 nucleotides
RR42_RS11140: RR42_RS11140 - ABC transporter permease, at 2,378,776 to 2,380,089
_RS11140
RR42_RS11145: RR42_RS11145 - ABC transporter permease, at 2,380,100 to 2,381,047
_RS11145
RR42_RS11150: RR42_RS11150 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,381,228 to 2,382,466
_RS11150
Position (kb)
2380
2381
2382 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2379.155 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.524 kb on + strand, within RR42_RS11140 at 2379.527 kb on + strand, within RR42_RS11140 at 2379.528 kb on - strand, within RR42_RS11140 at 2379.594 kb on + strand, within RR42_RS11140 at 2379.708 kb on - strand, within RR42_RS11140 at 2379.799 kb on - strand, within RR42_RS11140 at 2379.799 kb on - strand, within RR42_RS11140 at 2379.890 kb on + strand, within RR42_RS11140 at 2379.890 kb on + strand, within RR42_RS11140 at 2379.906 kb on + strand, within RR42_RS11140 at 2379.907 kb on - strand, within RR42_RS11140 at 2380.098 kb on + strand at 2380.098 kb on + strand at 2380.099 kb on - strand at 2380.206 kb on - strand, within RR42_RS11145 at 2380.338 kb on - strand, within RR42_RS11145 at 2380.415 kb on - strand, within RR42_RS11145 at 2380.415 kb on - strand, within RR42_RS11145 at 2380.415 kb on - strand, within RR42_RS11145 at 2380.499 kb on - strand, within RR42_RS11145 at 2380.582 kb on - strand, within RR42_RS11145 at 2380.582 kb on - strand, within RR42_RS11145 at 2380.653 kb on - strand, within RR42_RS11145 at 2380.716 kb on - strand, within RR42_RS11145 at 2380.743 kb on + strand, within RR42_RS11145 at 2380.759 kb on + strand, within RR42_RS11145 at 2380.760 kb on - strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.918 kb on + strand, within RR42_RS11145 at 2380.918 kb on + strand, within RR42_RS11145 at 2380.918 kb on + strand, within RR42_RS11145 at 2380.919 kb on - strand at 2380.920 kb on + strand, within RR42_RS11145 at 2380.920 kb on + strand, within RR42_RS11145 at 2380.920 kb on + strand, within RR42_RS11145 at 2380.920 kb on + strand, within RR42_RS11145 at 2380.921 kb on - strand, within RR42_RS11145 at 2380.921 kb on - strand, within RR42_RS11145 at 2381.008 kb on - strand at 2381.052 kb on + strand at 2381.052 kb on + strand at 2381.053 kb on - strand at 2381.174 kb on - strand at 2381.174 kb on - strand at 2381.174 kb on - strand at 2381.274 kb on + strand at 2381.523 kb on + strand, within RR42_RS11150 at 2381.524 kb on - strand, within RR42_RS11150 at 2381.524 kb on - strand, within RR42_RS11150 at 2381.524 kb on - strand, within RR42_RS11150 at 2381.524 kb on - strand, within RR42_RS11150 at 2381.578 kb on + strand, within RR42_RS11150 at 2381.613 kb on + strand, within RR42_RS11150 at 2381.613 kb on + strand, within RR42_RS11150 at 2381.613 kb on + strand, within RR42_RS11150 at 2381.614 kb on - strand, within RR42_RS11150 at 2381.617 kb on - strand, within RR42_RS11150 at 2381.625 kb on + strand, within RR42_RS11150 at 2381.625 kb on + strand, within RR42_RS11150 at 2381.625 kb on + strand, within RR42_RS11150 at 2381.625 kb on + strand, within RR42_RS11150 at 2381.626 kb on - strand, within RR42_RS11150 at 2381.626 kb on - strand, within RR42_RS11150 at 2381.626 kb on - strand, within RR42_RS11150 at 2381.626 kb on - strand, within RR42_RS11150 at 2381.685 kb on + strand, within RR42_RS11150 at 2381.688 kb on + strand, within RR42_RS11150 at 2381.688 kb on + strand, within RR42_RS11150 at 2381.688 kb on + strand, within RR42_RS11150 at 2381.689 kb on - strand, within RR42_RS11150 at 2381.689 kb on - strand, within RR42_RS11150 at 2381.695 kb on - strand, within RR42_RS11150 at 2381.695 kb on - strand, within RR42_RS11150 at 2381.695 kb on - strand, within RR42_RS11150 at 2381.697 kb on + strand, within RR42_RS11150 at 2381.698 kb on - strand, within RR42_RS11150 at 2381.698 kb on - strand, within RR42_RS11150 at 2381.698 kb on - strand, within RR42_RS11150 at 2381.698 kb on - strand, within RR42_RS11150 at 2381.929 kb on - strand, within RR42_RS11150 at 2381.946 kb on + strand, within RR42_RS11150 at 2381.979 kb on + strand, within RR42_RS11150 at 2381.980 kb on - strand, within RR42_RS11150 at 2381.980 kb on - strand, within RR42_RS11150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,379,155 + RR42_RS11140 0.29 -0.4 2,379,482 + RR42_RS11140 0.54 +0.6 2,379,482 + RR42_RS11140 0.54 -2.1 2,379,482 + RR42_RS11140 0.54 -0.9 2,379,482 + RR42_RS11140 0.54 -1.4 2,379,483 - RR42_RS11140 0.54 -1.5 2,379,483 - RR42_RS11140 0.54 -0.6 2,379,483 - RR42_RS11140 0.54 +1.1 2,379,524 + RR42_RS11140 0.57 -0.6 2,379,527 + RR42_RS11140 0.57 +1.0 2,379,528 - RR42_RS11140 0.57 -1.0 2,379,594 + RR42_RS11140 0.62 -1.0 2,379,708 - RR42_RS11140 0.71 -0.2 2,379,799 - RR42_RS11140 0.78 -2.8 2,379,799 - RR42_RS11140 0.78 -0.1 2,379,890 + RR42_RS11140 0.85 -1.0 2,379,890 + RR42_RS11140 0.85 -0.3 2,379,906 + RR42_RS11140 0.86 -0.7 2,379,907 - RR42_RS11140 0.86 -2.3 2,380,098 + +2.1 2,380,098 + +0.1 2,380,099 - +0.3 2,380,206 - RR42_RS11145 0.11 +1.6 2,380,338 - RR42_RS11145 0.25 -1.4 2,380,415 - RR42_RS11145 0.33 -0.4 2,380,415 - RR42_RS11145 0.33 -0.3 2,380,415 - RR42_RS11145 0.33 -0.0 2,380,499 - RR42_RS11145 0.42 -1.0 2,380,582 - RR42_RS11145 0.51 -0.6 2,380,582 - RR42_RS11145 0.51 +0.8 2,380,653 - RR42_RS11145 0.58 +1.6 2,380,716 - RR42_RS11145 0.65 -1.0 2,380,743 + RR42_RS11145 0.68 +1.9 2,380,759 + RR42_RS11145 0.70 +0.1 2,380,760 - RR42_RS11145 0.70 +0.4 2,380,785 + RR42_RS11145 0.72 -0.4 2,380,785 + RR42_RS11145 0.72 -2.5 2,380,785 + RR42_RS11145 0.72 +1.0 2,380,785 + RR42_RS11145 0.72 -0.4 2,380,918 + RR42_RS11145 0.86 -0.5 2,380,918 + RR42_RS11145 0.86 -1.8 2,380,918 + RR42_RS11145 0.86 +0.7 2,380,919 - +1.1 2,380,920 + RR42_RS11145 0.86 +1.3 2,380,920 + RR42_RS11145 0.86 -0.3 2,380,920 + RR42_RS11145 0.86 -1.6 2,380,920 + RR42_RS11145 0.86 +0.8 2,380,921 - RR42_RS11145 0.87 +0.1 2,380,921 - RR42_RS11145 0.87 +1.5 2,381,008 - -1.2 2,381,052 + +0.1 2,381,052 + +1.1 2,381,053 - -0.9 2,381,174 - +0.7 2,381,174 - +0.6 2,381,174 - +4.2 2,381,274 + -0.5 2,381,523 + RR42_RS11150 0.24 -1.7 2,381,524 - RR42_RS11150 0.24 +0.1 2,381,524 - RR42_RS11150 0.24 -0.2 2,381,524 - RR42_RS11150 0.24 -0.3 2,381,524 - RR42_RS11150 0.24 +1.1 2,381,578 + RR42_RS11150 0.28 -0.7 2,381,613 + RR42_RS11150 0.31 -0.4 2,381,613 + RR42_RS11150 0.31 -0.5 2,381,613 + RR42_RS11150 0.31 -0.8 2,381,614 - RR42_RS11150 0.31 +0.9 2,381,617 - RR42_RS11150 0.31 +1.2 2,381,625 + RR42_RS11150 0.32 +1.4 2,381,625 + RR42_RS11150 0.32 -2.0 2,381,625 + RR42_RS11150 0.32 +0.5 2,381,625 + RR42_RS11150 0.32 +0.2 2,381,626 - RR42_RS11150 0.32 -1.8 2,381,626 - RR42_RS11150 0.32 -0.3 2,381,626 - RR42_RS11150 0.32 +0.3 2,381,626 - RR42_RS11150 0.32 +0.7 2,381,685 + RR42_RS11150 0.37 +1.5 2,381,688 + RR42_RS11150 0.37 +0.6 2,381,688 + RR42_RS11150 0.37 +0.6 2,381,688 + RR42_RS11150 0.37 -1.1 2,381,689 - RR42_RS11150 0.37 -0.7 2,381,689 - RR42_RS11150 0.37 +2.0 2,381,695 - RR42_RS11150 0.38 +0.5 2,381,695 - RR42_RS11150 0.38 +0.8 2,381,695 - RR42_RS11150 0.38 +1.6 2,381,697 + RR42_RS11150 0.38 +0.9 2,381,698 - RR42_RS11150 0.38 +1.1 2,381,698 - RR42_RS11150 0.38 +0.0 2,381,698 - RR42_RS11150 0.38 +1.1 2,381,698 - RR42_RS11150 0.38 +1.2 2,381,929 - RR42_RS11150 0.57 +0.3 2,381,946 + RR42_RS11150 0.58 -0.8 2,381,979 + RR42_RS11150 0.61 +1.8 2,381,980 - RR42_RS11150 0.61 +1.9 2,381,980 - RR42_RS11150 0.61 -0.1
Or see this region's nucleotide sequence