Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11060 and RR42_RS11065 are separated by 174 nucleotides RR42_RS11065 and RR42_RS11070 are separated by 90 nucleotides RR42_RS11070 and RR42_RS11075 are separated by 70 nucleotides
RR42_RS11060: RR42_RS11060 - 6-aminohexanoate hydrolase, at 2,359,842 to 2,361,161
_RS11060
RR42_RS11065: RR42_RS11065 - phenylacetic acid degradation protein, at 2,361,336 to 2,361,779
_RS11065
RR42_RS11070: RR42_RS11070 - gluconate 5-dehydrogenase, at 2,361,870 to 2,362,652
_RS11070
RR42_RS11075: RR42_RS11075 - acyl-CoA dehydrogenase, at 2,362,723 to 2,364,573
_RS11075
Position (kb)
2361
2362 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2360.392 kb on + strand, within RR42_RS11060 at 2360.392 kb on + strand, within RR42_RS11060 at 2360.393 kb on - strand, within RR42_RS11060 at 2360.446 kb on + strand, within RR42_RS11060 at 2360.506 kb on + strand, within RR42_RS11060 at 2360.506 kb on + strand, within RR42_RS11060 at 2360.506 kb on + strand, within RR42_RS11060 at 2360.537 kb on - strand, within RR42_RS11060 at 2360.548 kb on + strand, within RR42_RS11060 at 2360.549 kb on - strand, within RR42_RS11060 at 2360.729 kb on + strand, within RR42_RS11060 at 2360.730 kb on - strand, within RR42_RS11060 at 2360.801 kb on + strand, within RR42_RS11060 at 2360.804 kb on - strand, within RR42_RS11060 at 2361.157 kb on + strand at 2361.157 kb on + strand at 2361.157 kb on + strand at 2361.157 kb on + strand at 2361.157 kb on + strand at 2361.158 kb on - strand at 2361.158 kb on - strand at 2361.158 kb on - strand at 2361.262 kb on - strand at 2361.313 kb on + strand at 2361.482 kb on + strand, within RR42_RS11065 at 2361.482 kb on + strand, within RR42_RS11065 at 2361.482 kb on + strand, within RR42_RS11065 at 2361.787 kb on - strand at 2361.787 kb on - strand at 2361.804 kb on - strand at 2361.804 kb on - strand at 2361.871 kb on + strand at 2361.871 kb on + strand at 2361.871 kb on + strand at 2361.872 kb on - strand at 2361.872 kb on - strand at 2361.873 kb on + strand at 2362.156 kb on + strand, within RR42_RS11070 at 2362.156 kb on + strand, within RR42_RS11070 at 2362.156 kb on + strand, within RR42_RS11070 at 2362.157 kb on - strand, within RR42_RS11070 at 2362.157 kb on - strand, within RR42_RS11070 at 2362.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 2,360,392 + RR42_RS11060 0.42 +1.5 2,360,392 + RR42_RS11060 0.42 -2.4 2,360,393 - RR42_RS11060 0.42 +1.2 2,360,446 + RR42_RS11060 0.46 -1.0 2,360,506 + RR42_RS11060 0.50 +0.3 2,360,506 + RR42_RS11060 0.50 -1.9 2,360,506 + RR42_RS11060 0.50 +1.5 2,360,537 - RR42_RS11060 0.53 +0.6 2,360,548 + RR42_RS11060 0.53 +2.1 2,360,549 - RR42_RS11060 0.54 -2.1 2,360,729 + RR42_RS11060 0.67 -0.1 2,360,730 - RR42_RS11060 0.67 -0.3 2,360,801 + RR42_RS11060 0.73 +2.6 2,360,804 - RR42_RS11060 0.73 -0.2 2,361,157 + +0.6 2,361,157 + +1.5 2,361,157 + -1.7 2,361,157 + +0.8 2,361,157 + +0.5 2,361,158 - +0.5 2,361,158 - -1.6 2,361,158 - +3.1 2,361,262 - -0.4 2,361,313 + +0.1 2,361,482 + RR42_RS11065 0.33 -0.6 2,361,482 + RR42_RS11065 0.33 -1.0 2,361,482 + RR42_RS11065 0.33 -1.3 2,361,787 - +0.3 2,361,787 - +0.5 2,361,804 - -1.2 2,361,804 - +0.9 2,361,871 + +0.5 2,361,871 + -0.6 2,361,871 + +1.4 2,361,872 - -0.6 2,361,872 - +0.9 2,361,873 + +1.7 2,362,156 + RR42_RS11070 0.37 -1.5 2,362,156 + RR42_RS11070 0.37 -0.4 2,362,156 + RR42_RS11070 0.37 +0.4 2,362,157 - RR42_RS11070 0.37 -1.8 2,362,157 - RR42_RS11070 0.37 -1.6 2,362,744 - -0.3
Or see this region's nucleotide sequence