Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS08345

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS08335 and RR42_RS08340 are separated by 10 nucleotidesRR42_RS08340 and RR42_RS08345 are separated by 3 nucleotidesRR42_RS08345 and RR42_RS08350 are separated by 1 nucleotidesRR42_RS08350 and RR42_RS08355 are separated by 97 nucleotides RR42_RS08335: RR42_RS08335 - hypothetical protein, at 1,764,200 to 1,765,069 _RS08335 RR42_RS08340: RR42_RS08340 - phage tail protein, at 1,765,080 to 1,765,481 _RS08340 RR42_RS08345: RR42_RS08345 - baseplate protein, at 1,765,485 to 1,766,609 _RS08345 RR42_RS08350: RR42_RS08350 - hypothetical protein, at 1,766,611 to 1,767,294 _RS08350 RR42_RS08355: RR42_RS08355 - hypothetical protein, at 1,767,392 to 1,768,036 _RS08355 Position (kb) 1765 1766 1767Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1764.527 kb on + strand, within RR42_RS08335at 1764.527 kb on + strand, within RR42_RS08335at 1764.620 kb on - strand, within RR42_RS08335at 1764.659 kb on + strand, within RR42_RS08335at 1764.660 kb on - strand, within RR42_RS08335at 1764.660 kb on - strand, within RR42_RS08335at 1764.707 kb on - strand, within RR42_RS08335at 1764.920 kb on + strand, within RR42_RS08335at 1764.921 kb on - strand, within RR42_RS08335at 1764.929 kb on + strand, within RR42_RS08335at 1764.930 kb on - strand, within RR42_RS08335at 1764.965 kb on + strand, within RR42_RS08335at 1764.965 kb on + strand, within RR42_RS08335at 1764.966 kb on - strand, within RR42_RS08335at 1765.092 kb on + strandat 1765.099 kb on - strandat 1765.101 kb on + strandat 1765.101 kb on + strandat 1765.101 kb on + strandat 1765.102 kb on - strandat 1765.263 kb on + strand, within RR42_RS08340at 1765.470 kb on - strandat 1765.741 kb on + strand, within RR42_RS08345at 1765.774 kb on - strand, within RR42_RS08345at 1765.876 kb on - strand, within RR42_RS08345at 1766.020 kb on - strand, within RR42_RS08345at 1766.197 kb on - strand, within RR42_RS08345at 1766.197 kb on - strand, within RR42_RS08345at 1766.478 kb on + strand, within RR42_RS08345at 1766.597 kb on + strandat 1766.657 kb on - strandat 1766.657 kb on - strandat 1766.657 kb on - strandat 1766.746 kb on + strand, within RR42_RS08350at 1766.876 kb on - strand, within RR42_RS08350at 1767.293 kb on - strandat 1767.377 kb on + strandat 1767.377 kb on + strandat 1767.377 kb on + strandat 1767.378 kb on - strandat 1767.378 kb on - strandat 1767.378 kb on - strandat 1767.378 kb on - strandat 1767.382 kb on - strandat 1767.382 kb on - strandat 1767.382 kb on - strandat 1767.395 kb on + strandat 1767.395 kb on + strandat 1767.396 kb on - strandat 1767.551 kb on + strand, within RR42_RS08355at 1767.608 kb on + strand, within RR42_RS08355at 1767.608 kb on + strand, within RR42_RS08355

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,764,527 + RR42_RS08335 0.38 +2.2
1,764,527 + RR42_RS08335 0.38 +2.5
1,764,620 - RR42_RS08335 0.48 +2.6
1,764,659 + RR42_RS08335 0.53 +3.1
1,764,660 - RR42_RS08335 0.53 +2.1
1,764,660 - RR42_RS08335 0.53 +2.7
1,764,707 - RR42_RS08335 0.58 +2.3
1,764,920 + RR42_RS08335 0.83 +1.4
1,764,921 - RR42_RS08335 0.83 +3.2
1,764,929 + RR42_RS08335 0.84 +1.8
1,764,930 - RR42_RS08335 0.84 +1.8
1,764,965 + RR42_RS08335 0.88 +3.9
1,764,965 + RR42_RS08335 0.88 +2.3
1,764,966 - RR42_RS08335 0.88 +2.2
1,765,092 + +3.7
1,765,099 - +2.6
1,765,101 + +1.8
1,765,101 + +2.9
1,765,101 + +1.6
1,765,102 - +2.1
1,765,263 + RR42_RS08340 0.46 +2.3
1,765,470 - +2.3
1,765,741 + RR42_RS08345 0.23 -1.8
1,765,774 - RR42_RS08345 0.26 +1.9
1,765,876 - RR42_RS08345 0.35 +2.2
1,766,020 - RR42_RS08345 0.48 +3.0
1,766,197 - RR42_RS08345 0.63 +1.8
1,766,197 - RR42_RS08345 0.63 +2.1
1,766,478 + RR42_RS08345 0.88 +3.9
1,766,597 + +2.3
1,766,657 - +2.6
1,766,657 - +1.0
1,766,657 - +2.3
1,766,746 + RR42_RS08350 0.20 +2.7
1,766,876 - RR42_RS08350 0.39 +2.7
1,767,293 - +2.6
1,767,377 + +1.1
1,767,377 + +2.9
1,767,377 + +2.1
1,767,378 - +3.4
1,767,378 - +2.8
1,767,378 - +3.2
1,767,378 - +4.5
1,767,382 - +1.9
1,767,382 - +3.2
1,767,382 - +1.2
1,767,395 + +1.9
1,767,395 + +1.8
1,767,396 - +2.5
1,767,551 + RR42_RS08355 0.25 +3.2
1,767,608 + RR42_RS08355 0.33 +2.8
1,767,608 + RR42_RS08355 0.33 +2.0

Or see this region's nucleotide sequence