Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS08325

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS08315 and RR42_RS08320 are separated by 4 nucleotidesRR42_RS08320 and RR42_RS08325 are separated by 6 nucleotidesRR42_RS08325 and RR42_RS08330 overlap by 4 nucleotidesRR42_RS08330 and RR42_RS08335 are separated by 87 nucleotides RR42_RS08315: RR42_RS08315 - hypothetical protein, at 1,759,652 to 1,761,589 _RS08315 RR42_RS08320: RR42_RS08320 - hypothetical protein, at 1,761,594 to 1,762,556 _RS08320 RR42_RS08325: RR42_RS08325 - hypothetical protein, at 1,762,563 to 1,763,687 _RS08325 RR42_RS08330: RR42_RS08330 - hypothetical protein, at 1,763,684 to 1,764,112 _RS08330 RR42_RS08335: RR42_RS08335 - hypothetical protein, at 1,764,200 to 1,765,069 _RS08335 Position (kb) 1762 1763 1764Strain fitness (log2 ratio) -1 0 1 2 3 4at 1761.599 kb on + strandat 1761.676 kb on + strandat 1761.676 kb on + strandat 1761.862 kb on - strand, within RR42_RS08320at 1762.098 kb on - strand, within RR42_RS08320at 1762.148 kb on + strand, within RR42_RS08320at 1762.671 kb on + strandat 1763.035 kb on - strand, within RR42_RS08325at 1763.037 kb on + strand, within RR42_RS08325at 1763.145 kb on + strand, within RR42_RS08325at 1763.146 kb on - strand, within RR42_RS08325at 1763.196 kb on + strand, within RR42_RS08325at 1763.196 kb on + strand, within RR42_RS08325at 1763.317 kb on - strand, within RR42_RS08325at 1763.464 kb on - strand, within RR42_RS08325at 1763.464 kb on - strand, within RR42_RS08325at 1763.583 kb on + strandat 1763.584 kb on - strandat 1763.584 kb on - strandat 1763.584 kb on - strandat 1763.584 kb on - strandat 1763.683 kb on + strandat 1763.684 kb on - strandat 1763.783 kb on + strand, within RR42_RS08330at 1764.139 kb on + strandat 1764.208 kb on - strandat 1764.527 kb on + strand, within RR42_RS08335at 1764.527 kb on + strand, within RR42_RS08335at 1764.620 kb on - strand, within RR42_RS08335at 1764.659 kb on + strand, within RR42_RS08335at 1764.660 kb on - strand, within RR42_RS08335at 1764.660 kb on - strand, within RR42_RS08335

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,761,599 + +2.7
1,761,676 + +2.4
1,761,676 + +2.4
1,761,862 - RR42_RS08320 0.28 +2.2
1,762,098 - RR42_RS08320 0.52 +2.6
1,762,148 + RR42_RS08320 0.58 +3.0
1,762,671 + +2.7
1,763,035 - RR42_RS08325 0.42 +0.8
1,763,037 + RR42_RS08325 0.42 +1.9
1,763,145 + RR42_RS08325 0.52 +1.8
1,763,146 - RR42_RS08325 0.52 +2.5
1,763,196 + RR42_RS08325 0.56 +2.6
1,763,196 + RR42_RS08325 0.56 +3.2
1,763,317 - RR42_RS08325 0.67 +2.2
1,763,464 - RR42_RS08325 0.80 +2.5
1,763,464 - RR42_RS08325 0.80 +2.3
1,763,583 + +1.8
1,763,584 - +1.2
1,763,584 - +4.0
1,763,584 - +1.6
1,763,584 - +3.2
1,763,683 + +1.4
1,763,684 - +2.4
1,763,783 + RR42_RS08330 0.23 +1.7
1,764,139 + +1.8
1,764,208 - +2.1
1,764,527 + RR42_RS08335 0.38 +2.2
1,764,527 + RR42_RS08335 0.38 +2.5
1,764,620 - RR42_RS08335 0.48 +2.6
1,764,659 + RR42_RS08335 0.53 +3.1
1,764,660 - RR42_RS08335 0.53 +2.1
1,764,660 - RR42_RS08335 0.53 +2.7

Or see this region's nucleotide sequence