Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08315 and RR42_RS08320 are separated by 4 nucleotides RR42_RS08320 and RR42_RS08325 are separated by 6 nucleotides RR42_RS08325 and RR42_RS08330 overlap by 4 nucleotides RR42_RS08330 and RR42_RS08335 are separated by 87 nucleotides
RR42_RS08315: RR42_RS08315 - hypothetical protein, at 1,759,652 to 1,761,589
_RS08315
RR42_RS08320: RR42_RS08320 - hypothetical protein, at 1,761,594 to 1,762,556
_RS08320
RR42_RS08325: RR42_RS08325 - hypothetical protein, at 1,762,563 to 1,763,687
_RS08325
RR42_RS08330: RR42_RS08330 - hypothetical protein, at 1,763,684 to 1,764,112
_RS08330
RR42_RS08335: RR42_RS08335 - hypothetical protein, at 1,764,200 to 1,765,069
_RS08335
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1761.599 kb on + strand at 1761.676 kb on + strand at 1761.676 kb on + strand at 1761.862 kb on - strand, within RR42_RS08320 at 1762.098 kb on - strand, within RR42_RS08320 at 1762.148 kb on + strand, within RR42_RS08320 at 1762.671 kb on + strand at 1763.035 kb on - strand, within RR42_RS08325 at 1763.037 kb on + strand, within RR42_RS08325 at 1763.145 kb on + strand, within RR42_RS08325 at 1763.146 kb on - strand, within RR42_RS08325 at 1763.196 kb on + strand, within RR42_RS08325 at 1763.196 kb on + strand, within RR42_RS08325 at 1763.317 kb on - strand, within RR42_RS08325 at 1763.464 kb on - strand, within RR42_RS08325 at 1763.464 kb on - strand, within RR42_RS08325 at 1763.583 kb on + strand at 1763.584 kb on - strand at 1763.584 kb on - strand at 1763.584 kb on - strand at 1763.584 kb on - strand at 1763.683 kb on + strand at 1763.684 kb on - strand at 1763.783 kb on + strand, within RR42_RS08330 at 1764.139 kb on + strand at 1764.208 kb on - strand at 1764.527 kb on + strand, within RR42_RS08335 at 1764.527 kb on + strand, within RR42_RS08335 at 1764.620 kb on - strand, within RR42_RS08335 at 1764.659 kb on + strand, within RR42_RS08335 at 1764.660 kb on - strand, within RR42_RS08335 at 1764.660 kb on - strand, within RR42_RS08335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,761,599 + +2.7 1,761,676 + +2.4 1,761,676 + +2.4 1,761,862 - RR42_RS08320 0.28 +2.2 1,762,098 - RR42_RS08320 0.52 +2.6 1,762,148 + RR42_RS08320 0.58 +3.0 1,762,671 + +2.7 1,763,035 - RR42_RS08325 0.42 +0.8 1,763,037 + RR42_RS08325 0.42 +1.9 1,763,145 + RR42_RS08325 0.52 +1.8 1,763,146 - RR42_RS08325 0.52 +2.5 1,763,196 + RR42_RS08325 0.56 +2.6 1,763,196 + RR42_RS08325 0.56 +3.2 1,763,317 - RR42_RS08325 0.67 +2.2 1,763,464 - RR42_RS08325 0.80 +2.5 1,763,464 - RR42_RS08325 0.80 +2.3 1,763,583 + +1.8 1,763,584 - +1.2 1,763,584 - +4.0 1,763,584 - +1.6 1,763,584 - +3.2 1,763,683 + +1.4 1,763,684 - +2.4 1,763,783 + RR42_RS08330 0.23 +1.7 1,764,139 + +1.8 1,764,208 - +2.1 1,764,527 + RR42_RS08335 0.38 +2.2 1,764,527 + RR42_RS08335 0.38 +2.5 1,764,620 - RR42_RS08335 0.48 +2.6 1,764,659 + RR42_RS08335 0.53 +3.1 1,764,660 - RR42_RS08335 0.53 +2.1 1,764,660 - RR42_RS08335 0.53 +2.7
Or see this region's nucleotide sequence