Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS08300

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS08290 and RR42_RS08295 are separated by 62 nucleotidesRR42_RS08295 and RR42_RS08300 overlap by 1 nucleotidesRR42_RS08300 and RR42_RS08305 are separated by 178 nucleotidesRR42_RS08305 and RR42_RS08310 overlap by 14 nucleotidesRR42_RS08310 and RR42_RS08315 are separated by 48 nucleotides RR42_RS08290: RR42_RS08290 - hypothetical protein, at 1,756,778 to 1,757,818 _RS08290 RR42_RS08295: RR42_RS08295 - hypothetical protein, at 1,757,881 to 1,758,321 _RS08295 RR42_RS08300: RR42_RS08300 - hypothetical protein, at 1,758,321 to 1,758,701 _RS08300 RR42_RS08305: RR42_RS08305 - phage protein, HK97 gp10 family, at 1,758,880 to 1,759,359 _RS08305 RR42_RS08310: RR42_RS08310 - hypothetical protein, at 1,759,346 to 1,759,603 _RS08310 RR42_RS08315: RR42_RS08315 - hypothetical protein, at 1,759,652 to 1,761,589 _RS08315 Position (kb) 1758 1759Strain fitness (log2 ratio) -1 0 1 2 3 4at 1757.427 kb on - strand, within RR42_RS08290at 1757.462 kb on + strand, within RR42_RS08290at 1757.462 kb on + strand, within RR42_RS08290at 1757.463 kb on - strand, within RR42_RS08290at 1757.463 kb on - strand, within RR42_RS08290at 1757.537 kb on + strand, within RR42_RS08290at 1757.537 kb on + strand, within RR42_RS08290at 1757.630 kb on + strand, within RR42_RS08290at 1757.631 kb on - strand, within RR42_RS08290at 1757.869 kb on + strandat 1757.870 kb on - strandat 1757.881 kb on - strandat 1757.895 kb on + strandat 1757.895 kb on + strandat 1758.109 kb on + strand, within RR42_RS08295at 1758.110 kb on - strand, within RR42_RS08295at 1758.133 kb on + strand, within RR42_RS08295at 1758.134 kb on - strand, within RR42_RS08295at 1758.174 kb on + strand, within RR42_RS08295at 1758.174 kb on + strand, within RR42_RS08295at 1758.507 kb on - strand, within RR42_RS08300at 1758.594 kb on + strand, within RR42_RS08300at 1758.594 kb on + strand, within RR42_RS08300at 1758.594 kb on + strand, within RR42_RS08300at 1758.598 kb on + strand, within RR42_RS08300at 1758.598 kb on + strand, within RR42_RS08300at 1758.727 kb on - strandat 1758.817 kb on + strandat 1759.383 kb on + strand, within RR42_RS08310at 1759.384 kb on - strand, within RR42_RS08310at 1759.398 kb on + strand, within RR42_RS08310at 1759.398 kb on + strand, within RR42_RS08310at 1759.398 kb on + strand, within RR42_RS08310at 1759.399 kb on - strand, within RR42_RS08310at 1759.417 kb on + strand, within RR42_RS08310at 1759.417 kb on + strand, within RR42_RS08310at 1759.637 kb on + strandat 1759.638 kb on - strandat 1759.639 kb on + strandat 1759.639 kb on + strandat 1759.639 kb on + strandat 1759.665 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,757,427 - RR42_RS08290 0.62 -1.2
1,757,462 + RR42_RS08290 0.66 +2.0
1,757,462 + RR42_RS08290 0.66 +2.2
1,757,463 - RR42_RS08290 0.66 +2.8
1,757,463 - RR42_RS08290 0.66 +2.3
1,757,537 + RR42_RS08290 0.73 +2.5
1,757,537 + RR42_RS08290 0.73 +2.1
1,757,630 + RR42_RS08290 0.82 +2.0
1,757,631 - RR42_RS08290 0.82 +2.6
1,757,869 + +1.1
1,757,870 - +2.5
1,757,881 - +2.9
1,757,895 + +3.7
1,757,895 + +2.4
1,758,109 + RR42_RS08295 0.52 +1.7
1,758,110 - RR42_RS08295 0.52 +2.0
1,758,133 + RR42_RS08295 0.57 +3.0
1,758,134 - RR42_RS08295 0.57 +2.9
1,758,174 + RR42_RS08295 0.66 +2.1
1,758,174 + RR42_RS08295 0.66 +3.2
1,758,507 - RR42_RS08300 0.49 +2.2
1,758,594 + RR42_RS08300 0.72 +2.5
1,758,594 + RR42_RS08300 0.72 +1.7
1,758,594 + RR42_RS08300 0.72 +3.1
1,758,598 + RR42_RS08300 0.73 +2.2
1,758,598 + RR42_RS08300 0.73 +3.1
1,758,727 - +2.5
1,758,817 + +3.5
1,759,383 + RR42_RS08310 0.14 -1.5
1,759,384 - RR42_RS08310 0.15 +2.2
1,759,398 + RR42_RS08310 0.20 +3.3
1,759,398 + RR42_RS08310 0.20 +1.7
1,759,398 + RR42_RS08310 0.20 +2.7
1,759,399 - RR42_RS08310 0.21 +2.8
1,759,417 + RR42_RS08310 0.28 +2.1
1,759,417 + RR42_RS08310 0.28 +1.8
1,759,637 + +1.4
1,759,638 - +3.2
1,759,639 + +2.6
1,759,639 + +3.8
1,759,639 + +3.5
1,759,665 - +0.5

Or see this region's nucleotide sequence