Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08125 and RR42_RS08130 are separated by 376 nucleotides RR42_RS08130 and RR42_RS08135 are separated by 65 nucleotides RR42_RS08135 and RR42_RS08140 are separated by 69 nucleotides
RR42_RS08125: RR42_RS08125 - hypothetical protein, at 1,734,431 to 1,734,685
_RS08125
RR42_RS08130: RR42_RS08130 - hypothetical protein, at 1,735,062 to 1,735,346
_RS08130
RR42_RS08135: RR42_RS08135 - hypothetical protein, at 1,735,412 to 1,736,032
_RS08135
RR42_RS08140: RR42_RS08140 - hypothetical protein, at 1,736,102 to 1,736,665
_RS08140
Position (kb)
1735
1736
1737 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1734.418 kb on + strand at 1734.418 kb on + strand at 1734.419 kb on - strand at 1734.432 kb on + strand at 1734.432 kb on + strand at 1734.433 kb on - strand at 1734.433 kb on - strand at 1734.532 kb on + strand, within RR42_RS08125 at 1734.532 kb on + strand, within RR42_RS08125 at 1734.532 kb on + strand, within RR42_RS08125 at 1734.532 kb on + strand, within RR42_RS08125 at 1734.533 kb on - strand, within RR42_RS08125 at 1734.533 kb on - strand, within RR42_RS08125 at 1734.536 kb on + strand, within RR42_RS08125 at 1734.536 kb on + strand, within RR42_RS08125 at 1734.537 kb on - strand, within RR42_RS08125 at 1734.537 kb on - strand, within RR42_RS08125 at 1734.537 kb on - strand, within RR42_RS08125 at 1734.537 kb on - strand, within RR42_RS08125 at 1734.537 kb on - strand, within RR42_RS08125 at 1734.605 kb on + strand, within RR42_RS08125 at 1734.606 kb on - strand, within RR42_RS08125 at 1734.606 kb on - strand, within RR42_RS08125 at 1734.606 kb on - strand, within RR42_RS08125 at 1734.609 kb on + strand, within RR42_RS08125 at 1734.609 kb on + strand, within RR42_RS08125 at 1734.609 kb on + strand, within RR42_RS08125 at 1734.609 kb on + strand, within RR42_RS08125 at 1734.620 kb on + strand, within RR42_RS08125 at 1734.621 kb on - strand, within RR42_RS08125 at 1734.621 kb on - strand, within RR42_RS08125 at 1734.695 kb on - strand at 1734.695 kb on - strand at 1734.697 kb on - strand at 1734.741 kb on + strand at 1734.793 kb on + strand at 1734.794 kb on - strand at 1734.827 kb on + strand at 1734.827 kb on + strand at 1734.829 kb on + strand at 1735.064 kb on - strand at 1735.180 kb on + strand, within RR42_RS08130 at 1735.180 kb on + strand, within RR42_RS08130 at 1735.181 kb on - strand, within RR42_RS08130 at 1735.212 kb on - strand, within RR42_RS08130 at 1735.212 kb on - strand, within RR42_RS08130 at 1735.346 kb on + strand at 1735.346 kb on + strand at 1735.346 kb on + strand at 1735.347 kb on - strand at 1735.360 kb on + strand at 1735.363 kb on + strand at 1735.363 kb on + strand at 1735.364 kb on - strand at 1735.364 kb on - strand at 1735.364 kb on - strand at 1735.364 kb on - strand at 1735.364 kb on - strand at 1735.364 kb on - strand at 1735.413 kb on + strand at 1735.449 kb on + strand at 1735.667 kb on + strand, within RR42_RS08135 at 1735.668 kb on - strand, within RR42_RS08135 at 1735.668 kb on - strand, within RR42_RS08135 at 1735.762 kb on + strand, within RR42_RS08135 at 1735.762 kb on + strand, within RR42_RS08135 at 1735.763 kb on - strand, within RR42_RS08135 at 1735.895 kb on - strand, within RR42_RS08135 at 1735.938 kb on + strand, within RR42_RS08135 at 1735.938 kb on + strand, within RR42_RS08135 at 1735.939 kb on - strand, within RR42_RS08135 at 1735.939 kb on - strand, within RR42_RS08135 at 1735.999 kb on + strand at 1736.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,734,418 + +2.1 1,734,418 + +1.7 1,734,419 - +2.4 1,734,432 + +0.3 1,734,432 + +1.8 1,734,433 - +1.7 1,734,433 - +1.8 1,734,532 + RR42_RS08125 0.40 +2.9 1,734,532 + RR42_RS08125 0.40 +2.1 1,734,532 + RR42_RS08125 0.40 +0.6 1,734,532 + RR42_RS08125 0.40 +2.8 1,734,533 - RR42_RS08125 0.40 +2.6 1,734,533 - RR42_RS08125 0.40 +2.5 1,734,536 + RR42_RS08125 0.41 +2.2 1,734,536 + RR42_RS08125 0.41 +2.8 1,734,537 - RR42_RS08125 0.42 +3.1 1,734,537 - RR42_RS08125 0.42 +2.9 1,734,537 - RR42_RS08125 0.42 +2.8 1,734,537 - RR42_RS08125 0.42 +3.4 1,734,537 - RR42_RS08125 0.42 +1.6 1,734,605 + RR42_RS08125 0.68 +0.1 1,734,606 - RR42_RS08125 0.69 -0.4 1,734,606 - RR42_RS08125 0.69 +2.3 1,734,606 - RR42_RS08125 0.69 +1.3 1,734,609 + RR42_RS08125 0.70 +1.0 1,734,609 + RR42_RS08125 0.70 +2.1 1,734,609 + RR42_RS08125 0.70 +1.3 1,734,609 + RR42_RS08125 0.70 +2.8 1,734,620 + RR42_RS08125 0.74 +2.0 1,734,621 - RR42_RS08125 0.75 +3.2 1,734,621 - RR42_RS08125 0.75 +1.5 1,734,695 - +2.8 1,734,695 - +2.1 1,734,697 - +2.2 1,734,741 + +1.4 1,734,793 + +2.5 1,734,794 - +0.9 1,734,827 + +2.4 1,734,827 + +0.8 1,734,829 + +2.4 1,735,064 - +2.7 1,735,180 + RR42_RS08130 0.41 +2.3 1,735,180 + RR42_RS08130 0.41 +3.1 1,735,181 - RR42_RS08130 0.42 +1.5 1,735,212 - RR42_RS08130 0.53 +2.3 1,735,212 - RR42_RS08130 0.53 +2.6 1,735,346 + +2.6 1,735,346 + +2.8 1,735,346 + +0.3 1,735,347 - +3.6 1,735,360 + +2.4 1,735,363 + +2.7 1,735,363 + +2.8 1,735,364 - +2.3 1,735,364 - +3.4 1,735,364 - +4.0 1,735,364 - +2.2 1,735,364 - +1.6 1,735,364 - +0.0 1,735,413 + +2.2 1,735,449 + +2.2 1,735,667 + RR42_RS08135 0.41 +2.7 1,735,668 - RR42_RS08135 0.41 +2.0 1,735,668 - RR42_RS08135 0.41 +2.0 1,735,762 + RR42_RS08135 0.56 +3.0 1,735,762 + RR42_RS08135 0.56 +1.9 1,735,763 - RR42_RS08135 0.57 +1.9 1,735,895 - RR42_RS08135 0.78 +0.8 1,735,938 + RR42_RS08135 0.85 +2.3 1,735,938 + RR42_RS08135 0.85 +2.4 1,735,939 - RR42_RS08135 0.85 +2.3 1,735,939 - RR42_RS08135 0.85 +1.9 1,735,999 + +2.6 1,736,019 - +1.7
Or see this region's nucleotide sequence