Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS08135

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS08125 and RR42_RS08130 are separated by 376 nucleotidesRR42_RS08130 and RR42_RS08135 are separated by 65 nucleotidesRR42_RS08135 and RR42_RS08140 are separated by 69 nucleotides RR42_RS08125: RR42_RS08125 - hypothetical protein, at 1,734,431 to 1,734,685 _RS08125 RR42_RS08130: RR42_RS08130 - hypothetical protein, at 1,735,062 to 1,735,346 _RS08130 RR42_RS08135: RR42_RS08135 - hypothetical protein, at 1,735,412 to 1,736,032 _RS08135 RR42_RS08140: RR42_RS08140 - hypothetical protein, at 1,736,102 to 1,736,665 _RS08140 Position (kb) 1735 1736 1737Strain fitness (log2 ratio) -1 0 1 2 3 4at 1734.418 kb on + strandat 1734.418 kb on + strandat 1734.419 kb on - strandat 1734.432 kb on + strandat 1734.432 kb on + strandat 1734.433 kb on - strandat 1734.433 kb on - strandat 1734.532 kb on + strand, within RR42_RS08125at 1734.532 kb on + strand, within RR42_RS08125at 1734.532 kb on + strand, within RR42_RS08125at 1734.532 kb on + strand, within RR42_RS08125at 1734.533 kb on - strand, within RR42_RS08125at 1734.533 kb on - strand, within RR42_RS08125at 1734.536 kb on + strand, within RR42_RS08125at 1734.536 kb on + strand, within RR42_RS08125at 1734.537 kb on - strand, within RR42_RS08125at 1734.537 kb on - strand, within RR42_RS08125at 1734.537 kb on - strand, within RR42_RS08125at 1734.537 kb on - strand, within RR42_RS08125at 1734.537 kb on - strand, within RR42_RS08125at 1734.605 kb on + strand, within RR42_RS08125at 1734.606 kb on - strand, within RR42_RS08125at 1734.606 kb on - strand, within RR42_RS08125at 1734.606 kb on - strand, within RR42_RS08125at 1734.609 kb on + strand, within RR42_RS08125at 1734.609 kb on + strand, within RR42_RS08125at 1734.609 kb on + strand, within RR42_RS08125at 1734.609 kb on + strand, within RR42_RS08125at 1734.620 kb on + strand, within RR42_RS08125at 1734.621 kb on - strand, within RR42_RS08125at 1734.621 kb on - strand, within RR42_RS08125at 1734.695 kb on - strandat 1734.695 kb on - strandat 1734.697 kb on - strandat 1734.741 kb on + strandat 1734.793 kb on + strandat 1734.794 kb on - strandat 1734.827 kb on + strandat 1734.827 kb on + strandat 1734.829 kb on + strandat 1735.064 kb on - strandat 1735.180 kb on + strand, within RR42_RS08130at 1735.180 kb on + strand, within RR42_RS08130at 1735.181 kb on - strand, within RR42_RS08130at 1735.212 kb on - strand, within RR42_RS08130at 1735.212 kb on - strand, within RR42_RS08130at 1735.346 kb on + strandat 1735.346 kb on + strandat 1735.346 kb on + strandat 1735.347 kb on - strandat 1735.360 kb on + strandat 1735.363 kb on + strandat 1735.363 kb on + strandat 1735.364 kb on - strandat 1735.364 kb on - strandat 1735.364 kb on - strandat 1735.364 kb on - strandat 1735.364 kb on - strandat 1735.364 kb on - strandat 1735.413 kb on + strandat 1735.449 kb on + strandat 1735.667 kb on + strand, within RR42_RS08135at 1735.668 kb on - strand, within RR42_RS08135at 1735.668 kb on - strand, within RR42_RS08135at 1735.762 kb on + strand, within RR42_RS08135at 1735.762 kb on + strand, within RR42_RS08135at 1735.763 kb on - strand, within RR42_RS08135at 1735.895 kb on - strand, within RR42_RS08135at 1735.938 kb on + strand, within RR42_RS08135at 1735.938 kb on + strand, within RR42_RS08135at 1735.939 kb on - strand, within RR42_RS08135at 1735.939 kb on - strand, within RR42_RS08135at 1735.999 kb on + strandat 1736.019 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,734,418 + +2.1
1,734,418 + +1.7
1,734,419 - +2.4
1,734,432 + +0.3
1,734,432 + +1.8
1,734,433 - +1.7
1,734,433 - +1.8
1,734,532 + RR42_RS08125 0.40 +2.9
1,734,532 + RR42_RS08125 0.40 +2.1
1,734,532 + RR42_RS08125 0.40 +0.6
1,734,532 + RR42_RS08125 0.40 +2.8
1,734,533 - RR42_RS08125 0.40 +2.6
1,734,533 - RR42_RS08125 0.40 +2.5
1,734,536 + RR42_RS08125 0.41 +2.2
1,734,536 + RR42_RS08125 0.41 +2.8
1,734,537 - RR42_RS08125 0.42 +3.1
1,734,537 - RR42_RS08125 0.42 +2.9
1,734,537 - RR42_RS08125 0.42 +2.8
1,734,537 - RR42_RS08125 0.42 +3.4
1,734,537 - RR42_RS08125 0.42 +1.6
1,734,605 + RR42_RS08125 0.68 +0.1
1,734,606 - RR42_RS08125 0.69 -0.4
1,734,606 - RR42_RS08125 0.69 +2.3
1,734,606 - RR42_RS08125 0.69 +1.3
1,734,609 + RR42_RS08125 0.70 +1.0
1,734,609 + RR42_RS08125 0.70 +2.1
1,734,609 + RR42_RS08125 0.70 +1.3
1,734,609 + RR42_RS08125 0.70 +2.8
1,734,620 + RR42_RS08125 0.74 +2.0
1,734,621 - RR42_RS08125 0.75 +3.2
1,734,621 - RR42_RS08125 0.75 +1.5
1,734,695 - +2.8
1,734,695 - +2.1
1,734,697 - +2.2
1,734,741 + +1.4
1,734,793 + +2.5
1,734,794 - +0.9
1,734,827 + +2.4
1,734,827 + +0.8
1,734,829 + +2.4
1,735,064 - +2.7
1,735,180 + RR42_RS08130 0.41 +2.3
1,735,180 + RR42_RS08130 0.41 +3.1
1,735,181 - RR42_RS08130 0.42 +1.5
1,735,212 - RR42_RS08130 0.53 +2.3
1,735,212 - RR42_RS08130 0.53 +2.6
1,735,346 + +2.6
1,735,346 + +2.8
1,735,346 + +0.3
1,735,347 - +3.6
1,735,360 + +2.4
1,735,363 + +2.7
1,735,363 + +2.8
1,735,364 - +2.3
1,735,364 - +3.4
1,735,364 - +4.0
1,735,364 - +2.2
1,735,364 - +1.6
1,735,364 - +0.0
1,735,413 + +2.2
1,735,449 + +2.2
1,735,667 + RR42_RS08135 0.41 +2.7
1,735,668 - RR42_RS08135 0.41 +2.0
1,735,668 - RR42_RS08135 0.41 +2.0
1,735,762 + RR42_RS08135 0.56 +3.0
1,735,762 + RR42_RS08135 0.56 +1.9
1,735,763 - RR42_RS08135 0.57 +1.9
1,735,895 - RR42_RS08135 0.78 +0.8
1,735,938 + RR42_RS08135 0.85 +2.3
1,735,938 + RR42_RS08135 0.85 +2.4
1,735,939 - RR42_RS08135 0.85 +2.3
1,735,939 - RR42_RS08135 0.85 +1.9
1,735,999 + +2.6
1,736,019 - +1.7

Or see this region's nucleotide sequence