Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07270 and RR42_RS07275 are separated by 347 nucleotides RR42_RS07275 and RR42_RS07280 are separated by 369 nucleotides
RR42_RS07270: RR42_RS07270 - NAD-glutamate dehydrogenase, at 1,546,204 to 1,551,084
_RS07270
RR42_RS07275: RR42_RS07275 - acetyl-CoA hydrolase, at 1,551,432 to 1,552,946
_RS07275
RR42_RS07280: RR42_RS07280 - hypothetical protein, at 1,553,316 to 1,553,699
_RS07280
Position (kb)
1551
1552
1553 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1550.847 kb on - strand at 1550.915 kb on + strand at 1550.966 kb on + strand at 1550.967 kb on - strand at 1550.969 kb on + strand at 1550.970 kb on - strand at 1550.970 kb on - strand at 1550.970 kb on - strand at 1551.184 kb on + strand at 1551.184 kb on + strand at 1551.185 kb on - strand at 1551.188 kb on + strand at 1551.189 kb on - strand at 1551.189 kb on - strand at 1551.189 kb on - strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.193 kb on + strand at 1551.194 kb on - strand at 1551.194 kb on - strand at 1551.200 kb on + strand at 1551.200 kb on + strand at 1551.200 kb on + strand at 1551.252 kb on + strand at 1551.253 kb on - strand at 1551.314 kb on - strand at 1551.314 kb on - strand at 1551.314 kb on - strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.428 kb on + strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.429 kb on - strand at 1551.435 kb on + strand at 1551.435 kb on + strand at 1551.436 kb on - strand at 1551.446 kb on + strand at 1551.447 kb on - strand at 1551.805 kb on + strand, within RR42_RS07275 at 1551.932 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.966 kb on + strand, within RR42_RS07275 at 1551.967 kb on - strand, within RR42_RS07275 at 1551.967 kb on - strand, within RR42_RS07275 at 1551.998 kb on + strand, within RR42_RS07275 at 1551.999 kb on - strand, within RR42_RS07275 at 1552.041 kb on - strand, within RR42_RS07275 at 1552.094 kb on + strand, within RR42_RS07275 at 1552.094 kb on + strand, within RR42_RS07275 at 1552.287 kb on + strand, within RR42_RS07275 at 1552.287 kb on + strand, within RR42_RS07275 at 1552.288 kb on - strand, within RR42_RS07275 at 1552.384 kb on - strand, within RR42_RS07275 at 1552.414 kb on - strand, within RR42_RS07275 at 1552.514 kb on + strand, within RR42_RS07275 at 1552.515 kb on - strand, within RR42_RS07275 at 1552.518 kb on + strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.519 kb on - strand, within RR42_RS07275 at 1552.797 kb on + strand at 1552.798 kb on - strand at 1552.798 kb on - strand at 1552.818 kb on + strand at 1552.818 kb on + strand at 1552.818 kb on + strand at 1552.819 kb on - strand at 1552.819 kb on - strand at 1552.850 kb on + strand at 1552.962 kb on + strand at 1552.962 kb on + strand at 1553.007 kb on + strand at 1553.008 kb on - strand at 1553.051 kb on + strand at 1553.051 kb on + strand at 1553.052 kb on - strand at 1553.052 kb on - strand at 1553.113 kb on - strand at 1553.137 kb on + strand at 1553.137 kb on + strand at 1553.137 kb on + strand at 1553.138 kb on - strand at 1553.138 kb on - strand at 1553.138 kb on - strand at 1553.343 kb on - strand at 1553.344 kb on + strand at 1553.344 kb on + strand at 1553.345 kb on - strand at 1553.345 kb on - strand at 1553.381 kb on - strand, within RR42_RS07280 at 1553.416 kb on + strand, within RR42_RS07280 at 1553.452 kb on + strand, within RR42_RS07280 at 1553.524 kb on + strand, within RR42_RS07280 at 1553.580 kb on - strand, within RR42_RS07280 at 1553.580 kb on - strand, within RR42_RS07280 at 1553.614 kb on + strand, within RR42_RS07280 at 1553.614 kb on + strand, within RR42_RS07280 at 1553.614 kb on + strand, within RR42_RS07280 at 1553.615 kb on - strand, within RR42_RS07280 at 1553.615 kb on - strand, within RR42_RS07280 at 1553.716 kb on - strand at 1553.716 kb on - strand at 1553.794 kb on - strand at 1553.821 kb on - strand at 1553.838 kb on + strand at 1553.838 kb on + strand at 1553.838 kb on + strand at 1553.838 kb on + strand at 1553.839 kb on - strand at 1553.851 kb on - strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.910 kb on + strand at 1553.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,550,847 - +0.5 1,550,915 + +0.1 1,550,966 + -0.3 1,550,967 - -0.3 1,550,969 + +0.1 1,550,970 - -0.0 1,550,970 - +0.6 1,550,970 - -1.0 1,551,184 + -0.9 1,551,184 + +1.3 1,551,185 - +0.6 1,551,188 + -1.5 1,551,189 - +0.6 1,551,189 - -0.9 1,551,189 - -0.4 1,551,193 + -1.1 1,551,193 + -0.7 1,551,193 + -1.5 1,551,193 + -0.1 1,551,194 - -1.2 1,551,194 - -1.7 1,551,200 + +0.1 1,551,200 + -0.2 1,551,200 + -0.9 1,551,252 + -0.7 1,551,253 - +2.6 1,551,314 - -0.3 1,551,314 - -1.7 1,551,314 - +1.6 1,551,428 + -0.8 1,551,428 + +1.3 1,551,428 + -0.5 1,551,428 + -1.2 1,551,428 + -0.1 1,551,428 + -1.7 1,551,428 + -0.4 1,551,428 + -1.1 1,551,429 - +0.2 1,551,429 - +0.1 1,551,429 - -0.5 1,551,429 - -2.5 1,551,429 - +4.9 1,551,435 + -0.5 1,551,435 + -2.4 1,551,436 - -0.0 1,551,446 + -0.1 1,551,447 - -1.6 1,551,805 + RR42_RS07275 0.25 -1.0 1,551,932 + RR42_RS07275 0.33 -1.1 1,551,966 + RR42_RS07275 0.35 -0.3 1,551,966 + RR42_RS07275 0.35 -0.5 1,551,966 + RR42_RS07275 0.35 -1.0 1,551,966 + RR42_RS07275 0.35 -1.5 1,551,967 - RR42_RS07275 0.35 -0.9 1,551,967 - RR42_RS07275 0.35 -0.0 1,551,998 + RR42_RS07275 0.37 +2.3 1,551,999 - RR42_RS07275 0.37 -0.3 1,552,041 - RR42_RS07275 0.40 +1.7 1,552,094 + RR42_RS07275 0.44 +1.9 1,552,094 + RR42_RS07275 0.44 -0.9 1,552,287 + RR42_RS07275 0.56 -2.6 1,552,287 + RR42_RS07275 0.56 -1.1 1,552,288 - RR42_RS07275 0.57 -0.8 1,552,384 - RR42_RS07275 0.63 +0.1 1,552,414 - RR42_RS07275 0.65 +0.9 1,552,514 + RR42_RS07275 0.71 -0.8 1,552,515 - RR42_RS07275 0.71 -1.6 1,552,518 + RR42_RS07275 0.72 -0.4 1,552,519 - RR42_RS07275 0.72 +0.5 1,552,519 - RR42_RS07275 0.72 +0.3 1,552,519 - RR42_RS07275 0.72 -0.7 1,552,519 - RR42_RS07275 0.72 -0.1 1,552,519 - RR42_RS07275 0.72 -0.5 1,552,797 + +0.1 1,552,798 - -1.4 1,552,798 - -0.1 1,552,818 + -0.9 1,552,818 + +0.7 1,552,818 + +0.3 1,552,819 - -0.6 1,552,819 - -1.1 1,552,850 + -2.1 1,552,962 + -0.1 1,552,962 + -2.0 1,553,007 + -0.9 1,553,008 - -2.3 1,553,051 + -0.4 1,553,051 + -0.1 1,553,052 - -0.6 1,553,052 - -0.7 1,553,113 - +1.3 1,553,137 + -2.1 1,553,137 + -0.3 1,553,137 + -1.6 1,553,138 - +2.3 1,553,138 - +1.5 1,553,138 - +1.7 1,553,343 - -0.7 1,553,344 + -0.9 1,553,344 + -0.2 1,553,345 - -1.3 1,553,345 - -0.5 1,553,381 - RR42_RS07280 0.17 -2.9 1,553,416 + RR42_RS07280 0.26 +1.5 1,553,452 + RR42_RS07280 0.35 +0.1 1,553,524 + RR42_RS07280 0.54 +0.6 1,553,580 - RR42_RS07280 0.69 -1.5 1,553,580 - RR42_RS07280 0.69 -1.4 1,553,614 + RR42_RS07280 0.78 -0.4 1,553,614 + RR42_RS07280 0.78 +1.3 1,553,614 + RR42_RS07280 0.78 -1.9 1,553,615 - RR42_RS07280 0.78 -0.8 1,553,615 - RR42_RS07280 0.78 -0.6 1,553,716 - -0.9 1,553,716 - +0.3 1,553,794 - -0.8 1,553,821 - -1.5 1,553,838 + -1.3 1,553,838 + -2.7 1,553,838 + -1.5 1,553,838 + -0.3 1,553,839 - -2.4 1,553,851 - +0.2 1,553,910 + -0.4 1,553,910 + -1.1 1,553,910 + +0.4 1,553,910 + +0.1 1,553,910 + -2.2 1,553,910 + -1.0 1,553,911 - -1.1
Or see this region's nucleotide sequence