Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07085 and RR42_RS07090 are separated by 24 nucleotides RR42_RS07090 and RR42_RS07095 are separated by 210 nucleotides RR42_RS07095 and RR42_RS07100 overlap by 1 nucleotides
RR42_RS07085: RR42_RS07085 - hypothetical protein, at 1,521,245 to 1,523,128
_RS07085
RR42_RS07090: RR42_RS07090 - hypothetical protein, at 1,523,153 to 1,523,551
_RS07090
RR42_RS07095: RR42_RS07095 - hypothetical protein, at 1,523,762 to 1,524,253
_RS07095
RR42_RS07100: RR42_RS07100 - hypothetical protein, at 1,524,253 to 1,524,762
_RS07100
Position (kb)
1523
1524 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1522.267 kb on + strand, within RR42_RS07085 at 1522.284 kb on + strand, within RR42_RS07085 at 1522.285 kb on - strand, within RR42_RS07085 at 1522.300 kb on + strand, within RR42_RS07085 at 1522.300 kb on + strand, within RR42_RS07085 at 1522.301 kb on - strand, within RR42_RS07085 at 1522.334 kb on - strand, within RR42_RS07085 at 1522.790 kb on + strand, within RR42_RS07085 at 1522.845 kb on - strand, within RR42_RS07085 at 1522.916 kb on - strand, within RR42_RS07085 at 1523.119 kb on + strand at 1523.155 kb on - strand at 1523.356 kb on + strand, within RR42_RS07090 at 1523.386 kb on + strand, within RR42_RS07090 at 1523.522 kb on + strand at 1523.522 kb on + strand at 1523.522 kb on + strand at 1523.614 kb on + strand at 1523.614 kb on + strand at 1523.615 kb on - strand at 1523.628 kb on - strand at 1523.645 kb on + strand at 1523.707 kb on + strand at 1523.707 kb on + strand at 1523.732 kb on + strand at 1523.744 kb on + strand at 1523.745 kb on - strand at 1523.745 kb on - strand at 1523.746 kb on + strand at 1523.783 kb on + strand at 1523.798 kb on + strand at 1523.798 kb on + strand at 1523.799 kb on - strand at 1523.988 kb on + strand, within RR42_RS07095 at 1523.989 kb on - strand, within RR42_RS07095 at 1523.989 kb on - strand, within RR42_RS07095 at 1523.989 kb on - strand, within RR42_RS07095 at 1524.002 kb on - strand, within RR42_RS07095 at 1524.026 kb on - strand, within RR42_RS07095 at 1524.026 kb on - strand, within RR42_RS07095 at 1524.026 kb on - strand, within RR42_RS07095 at 1524.086 kb on + strand, within RR42_RS07095 at 1524.087 kb on - strand, within RR42_RS07095 at 1524.209 kb on + strand at 1524.209 kb on + strand at 1524.209 kb on + strand at 1524.209 kb on + strand at 1524.209 kb on + strand at 1524.210 kb on - strand at 1524.210 kb on - strand at 1524.210 kb on - strand at 1524.269 kb on + strand at 1524.269 kb on + strand at 1524.334 kb on - strand, within RR42_RS07100 at 1524.472 kb on + strand, within RR42_RS07100 at 1524.472 kb on + strand, within RR42_RS07100 at 1524.473 kb on - strand, within RR42_RS07100 at 1524.473 kb on - strand, within RR42_RS07100 at 1524.487 kb on + strand, within RR42_RS07100 at 1524.487 kb on + strand, within RR42_RS07100 at 1524.488 kb on - strand, within RR42_RS07100 at 1524.488 kb on - strand, within RR42_RS07100 at 1524.488 kb on - strand, within RR42_RS07100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,522,267 + RR42_RS07085 0.54 +0.5 1,522,284 + RR42_RS07085 0.55 +0.3 1,522,285 - RR42_RS07085 0.55 -0.1 1,522,300 + RR42_RS07085 0.56 -1.5 1,522,300 + RR42_RS07085 0.56 -0.5 1,522,301 - RR42_RS07085 0.56 -1.0 1,522,334 - RR42_RS07085 0.58 -1.1 1,522,790 + RR42_RS07085 0.82 -0.5 1,522,845 - RR42_RS07085 0.85 +0.1 1,522,916 - RR42_RS07085 0.89 -0.4 1,523,119 + -0.8 1,523,155 - -0.2 1,523,356 + RR42_RS07090 0.51 -1.3 1,523,386 + RR42_RS07090 0.58 +0.3 1,523,522 + -2.7 1,523,522 + -2.2 1,523,522 + +1.0 1,523,614 + +3.2 1,523,614 + -1.4 1,523,615 - -1.0 1,523,628 - -0.2 1,523,645 + -0.2 1,523,707 + +1.2 1,523,707 + -1.4 1,523,732 + +1.5 1,523,744 + -1.5 1,523,745 - -0.9 1,523,745 - -0.9 1,523,746 + +1.5 1,523,783 + +0.4 1,523,798 + +0.9 1,523,798 + +0.5 1,523,799 - +0.3 1,523,988 + RR42_RS07095 0.46 -0.9 1,523,989 - RR42_RS07095 0.46 -0.6 1,523,989 - RR42_RS07095 0.46 +0.1 1,523,989 - RR42_RS07095 0.46 -2.3 1,524,002 - RR42_RS07095 0.49 -1.3 1,524,026 - RR42_RS07095 0.54 -0.6 1,524,026 - RR42_RS07095 0.54 +1.5 1,524,026 - RR42_RS07095 0.54 -1.1 1,524,086 + RR42_RS07095 0.66 -0.5 1,524,087 - RR42_RS07095 0.66 +0.1 1,524,209 + +0.7 1,524,209 + -1.0 1,524,209 + -0.0 1,524,209 + +0.3 1,524,209 + -0.1 1,524,210 - +1.0 1,524,210 - +0.5 1,524,210 - +1.1 1,524,269 + -2.1 1,524,269 + -0.7 1,524,334 - RR42_RS07100 0.16 +1.4 1,524,472 + RR42_RS07100 0.43 -0.8 1,524,472 + RR42_RS07100 0.43 +0.7 1,524,473 - RR42_RS07100 0.43 -1.7 1,524,473 - RR42_RS07100 0.43 -0.2 1,524,487 + RR42_RS07100 0.46 +0.7 1,524,487 + RR42_RS07100 0.46 -1.6 1,524,488 - RR42_RS07100 0.46 -1.7 1,524,488 - RR42_RS07100 0.46 -1.1 1,524,488 - RR42_RS07100 0.46 +1.6
Or see this region's nucleotide sequence