Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS06635

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS06625 and RR42_RS06630 are separated by 69 nucleotidesRR42_RS06630 and RR42_RS06635 are separated by 124 nucleotidesRR42_RS06635 and RR42_RS06640 are separated by 56 nucleotidesRR42_RS06640 and RR42_RS06645 are separated by 106 nucleotides RR42_RS06625: RR42_RS06625 - phenylalanyl-tRNA synthetase subunit beta, at 1,445,604 to 1,448,051 _RS06625 RR42_RS06630: RR42_RS06630 - integration host factor subunit alpha, at 1,448,121 to 1,448,537 _RS06630 RR42_RS06635: RR42_RS06635 - MerR family transcriptional regulator, at 1,448,662 to 1,449,093 _RS06635 RR42_RS06640: RR42_RS06640 - tRNA-Pro, at 1,449,150 to 1,449,226 _RS06640 RR42_RS06645: RR42_RS06645 - hypothetical protein, at 1,449,333 to 1,450,430 _RS06645 Position (kb) 1448 1449 1450Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1448.139 kb on + strandat 1448.201 kb on + strand, within RR42_RS06630at 1448.201 kb on + strand, within RR42_RS06630at 1448.583 kb on + strandat 1448.584 kb on - strandat 1448.661 kb on + strandat 1448.661 kb on + strandat 1448.661 kb on + strandat 1448.661 kb on + strandat 1448.716 kb on + strand, within RR42_RS06635at 1448.717 kb on - strand, within RR42_RS06635at 1448.717 kb on - strand, within RR42_RS06635at 1448.830 kb on + strand, within RR42_RS06635at 1448.830 kb on + strand, within RR42_RS06635at 1448.830 kb on + strand, within RR42_RS06635at 1449.136 kb on + strandat 1449.228 kb on + strandat 1449.255 kb on - strandat 1449.311 kb on + strandat 1449.312 kb on - strandat 1449.312 kb on - strandat 1449.328 kb on - strandat 1449.328 kb on - strandat 1449.345 kb on + strandat 1449.391 kb on + strandat 1449.391 kb on + strandat 1449.391 kb on + strandat 1449.416 kb on + strandat 1449.504 kb on + strand, within RR42_RS06645at 1449.504 kb on + strand, within RR42_RS06645at 1449.505 kb on - strand, within RR42_RS06645at 1449.601 kb on + strand, within RR42_RS06645at 1449.602 kb on - strand, within RR42_RS06645at 1449.602 kb on - strand, within RR42_RS06645at 1449.796 kb on + strand, within RR42_RS06645at 1449.797 kb on - strand, within RR42_RS06645at 1449.797 kb on - strand, within RR42_RS06645at 1449.904 kb on - strand, within RR42_RS06645at 1450.016 kb on + strand, within RR42_RS06645at 1450.021 kb on + strand, within RR42_RS06645at 1450.022 kb on - strand, within RR42_RS06645at 1450.022 kb on - strand, within RR42_RS06645

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,448,139 + +1.0
1,448,201 + RR42_RS06630 0.19 +1.2
1,448,201 + RR42_RS06630 0.19 +2.3
1,448,583 + -3.0
1,448,584 - +0.4
1,448,661 + -0.1
1,448,661 + +0.5
1,448,661 + +0.2
1,448,661 + -1.6
1,448,716 + RR42_RS06635 0.12 -0.3
1,448,717 - RR42_RS06635 0.13 -1.8
1,448,717 - RR42_RS06635 0.13 -0.5
1,448,830 + RR42_RS06635 0.39 -1.6
1,448,830 + RR42_RS06635 0.39 -0.6
1,448,830 + RR42_RS06635 0.39 +0.2
1,449,136 + -0.8
1,449,228 + -3.3
1,449,255 - +0.5
1,449,311 + -1.8
1,449,312 - +0.5
1,449,312 - +0.0
1,449,328 - -1.7
1,449,328 - -1.3
1,449,345 + +0.9
1,449,391 + +0.6
1,449,391 + +0.9
1,449,391 + +0.7
1,449,416 + +0.5
1,449,504 + RR42_RS06645 0.16 +1.3
1,449,504 + RR42_RS06645 0.16 -1.6
1,449,505 - RR42_RS06645 0.16 +0.4
1,449,601 + RR42_RS06645 0.24 +2.7
1,449,602 - RR42_RS06645 0.24 +0.2
1,449,602 - RR42_RS06645 0.24 +0.0
1,449,796 + RR42_RS06645 0.42 -0.5
1,449,797 - RR42_RS06645 0.42 -0.1
1,449,797 - RR42_RS06645 0.42 +1.9
1,449,904 - RR42_RS06645 0.52 -0.5
1,450,016 + RR42_RS06645 0.62 +0.7
1,450,021 + RR42_RS06645 0.63 -0.4
1,450,022 - RR42_RS06645 0.63 -1.1
1,450,022 - RR42_RS06645 0.63 +0.3

Or see this region's nucleotide sequence