Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS06355

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS06350 and RR42_RS06355 overlap by 4 nucleotidesRR42_RS06355 and RR42_RS06360 are separated by 114 nucleotides RR42_RS06350: RR42_RS06350 - cysteinyl-tRNA synthetase, at 1,392,256 to 1,393,644 _RS06350 RR42_RS06355: RR42_RS06355 - Fe-S cluster assembly protein HesB, at 1,393,641 to 1,394,567 _RS06355 RR42_RS06360: RR42_RS06360 - acetyl-CoA carboxylase subunit alpha, at 1,394,682 to 1,395,653 _RS06360 Position (kb) 1393 1394 1395Strain fitness (log2 ratio) -1 0 1 2at 1394.028 kb on + strand, within RR42_RS06355at 1394.028 kb on + strand, within RR42_RS06355at 1394.233 kb on - strand, within RR42_RS06355at 1394.282 kb on + strand, within RR42_RS06355at 1394.282 kb on + strand, within RR42_RS06355at 1394.282 kb on + strand, within RR42_RS06355at 1394.282 kb on + strand, within RR42_RS06355at 1394.283 kb on - strand, within RR42_RS06355at 1394.442 kb on + strand, within RR42_RS06355at 1394.526 kb on + strandat 1394.527 kb on - strandat 1394.527 kb on - strandat 1394.527 kb on - strandat 1394.562 kb on + strandat 1394.562 kb on + strandat 1394.562 kb on + strandat 1394.562 kb on + strandat 1394.565 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,394,028 + RR42_RS06355 0.42 +0.4
1,394,028 + RR42_RS06355 0.42 -1.1
1,394,233 - RR42_RS06355 0.64 +0.1
1,394,282 + RR42_RS06355 0.69 +0.2
1,394,282 + RR42_RS06355 0.69 -0.6
1,394,282 + RR42_RS06355 0.69 -1.0
1,394,282 + RR42_RS06355 0.69 +0.3
1,394,283 - RR42_RS06355 0.69 +1.2
1,394,442 + RR42_RS06355 0.86 -1.6
1,394,526 + -0.6
1,394,527 - +0.5
1,394,527 - +2.0
1,394,527 - +1.6
1,394,562 + -0.3
1,394,562 + -1.6
1,394,562 + -0.1
1,394,562 + -1.5
1,394,565 + +0.4

Or see this region's nucleotide sequence