Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06175 and RR42_RS06180 are separated by 83 nucleotides RR42_RS06180 and RR42_RS06185 are separated by 123 nucleotides RR42_RS06185 and RR42_RS06190 are separated by 126 nucleotides
RR42_RS06175: RR42_RS06175 - enolase, at 1,356,578 to 1,357,867
_RS06175
RR42_RS06180: RR42_RS06180 - cell division protein FtsB, at 1,357,951 to 1,358,289
_RS06180
RR42_RS06185: RR42_RS06185 - hypothetical protein, at 1,358,413 to 1,358,814
_RS06185
RR42_RS06190: RR42_RS06190 - molecular chaperone Hsp33, at 1,358,941 to 1,359,969
_RS06190
Position (kb)
1358
1359 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1357.853 kb on + strand at 1357.854 kb on - strand at 1358.287 kb on + strand at 1358.287 kb on + strand at 1358.405 kb on - strand at 1358.414 kb on + strand at 1358.414 kb on + strand at 1358.414 kb on + strand at 1358.424 kb on - strand at 1358.514 kb on - strand, within RR42_RS06185 at 1358.526 kb on + strand, within RR42_RS06185 at 1358.527 kb on - strand, within RR42_RS06185 at 1358.543 kb on + strand, within RR42_RS06185 at 1358.543 kb on + strand, within RR42_RS06185 at 1358.543 kb on + strand, within RR42_RS06185 at 1358.543 kb on + strand, within RR42_RS06185 at 1358.543 kb on + strand, within RR42_RS06185 at 1358.544 kb on - strand, within RR42_RS06185 at 1358.544 kb on - strand, within RR42_RS06185 at 1358.544 kb on - strand, within RR42_RS06185 at 1358.544 kb on - strand, within RR42_RS06185 at 1358.544 kb on - strand, within RR42_RS06185 at 1358.544 kb on - strand, within RR42_RS06185 at 1358.546 kb on + strand, within RR42_RS06185 at 1358.546 kb on + strand, within RR42_RS06185 at 1358.546 kb on + strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.547 kb on - strand, within RR42_RS06185 at 1358.614 kb on + strand, within RR42_RS06185 at 1358.615 kb on - strand, within RR42_RS06185 at 1359.029 kb on + strand at 1359.226 kb on + strand, within RR42_RS06190 at 1359.226 kb on + strand, within RR42_RS06190 at 1359.489 kb on + strand, within RR42_RS06190 at 1359.489 kb on + strand, within RR42_RS06190 at 1359.489 kb on + strand, within RR42_RS06190 at 1359.489 kb on + strand, within RR42_RS06190 at 1359.490 kb on - strand, within RR42_RS06190 at 1359.490 kb on - strand, within RR42_RS06190 at 1359.490 kb on - strand, within RR42_RS06190 at 1359.490 kb on - strand, within RR42_RS06190 at 1359.490 kb on - strand, within RR42_RS06190 at 1359.491 kb on + strand, within RR42_RS06190 at 1359.491 kb on + strand, within RR42_RS06190 at 1359.491 kb on + strand, within RR42_RS06190 at 1359.491 kb on + strand, within RR42_RS06190 at 1359.491 kb on + strand, within RR42_RS06190 at 1359.492 kb on - strand, within RR42_RS06190 at 1359.492 kb on - strand, within RR42_RS06190 at 1359.492 kb on - strand, within RR42_RS06190 at 1359.492 kb on - strand, within RR42_RS06190 at 1359.492 kb on - strand, within RR42_RS06190 at 1359.492 kb on - strand, within RR42_RS06190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,357,853 + -0.2 1,357,854 - -1.3 1,358,287 + +0.6 1,358,287 + +0.1 1,358,405 - -2.0 1,358,414 + -0.9 1,358,414 + -0.3 1,358,414 + +0.4 1,358,424 - -1.1 1,358,514 - RR42_RS06185 0.25 +0.2 1,358,526 + RR42_RS06185 0.28 -2.6 1,358,527 - RR42_RS06185 0.28 -1.7 1,358,543 + RR42_RS06185 0.32 -1.2 1,358,543 + RR42_RS06185 0.32 +0.1 1,358,543 + RR42_RS06185 0.32 +3.9 1,358,543 + RR42_RS06185 0.32 -0.8 1,358,543 + RR42_RS06185 0.32 +1.1 1,358,544 - RR42_RS06185 0.33 -2.5 1,358,544 - RR42_RS06185 0.33 +1.4 1,358,544 - RR42_RS06185 0.33 +0.1 1,358,544 - RR42_RS06185 0.33 +0.7 1,358,544 - RR42_RS06185 0.33 -1.6 1,358,544 - RR42_RS06185 0.33 -0.6 1,358,546 + RR42_RS06185 0.33 -1.0 1,358,546 + RR42_RS06185 0.33 -0.7 1,358,546 + RR42_RS06185 0.33 +0.5 1,358,547 - RR42_RS06185 0.33 -1.5 1,358,547 - RR42_RS06185 0.33 -0.2 1,358,547 - RR42_RS06185 0.33 +0.1 1,358,547 - RR42_RS06185 0.33 -0.7 1,358,547 - RR42_RS06185 0.33 -1.5 1,358,547 - RR42_RS06185 0.33 +1.0 1,358,547 - RR42_RS06185 0.33 -1.0 1,358,614 + RR42_RS06185 0.50 -0.9 1,358,615 - RR42_RS06185 0.50 -0.8 1,359,029 + +1.0 1,359,226 + RR42_RS06190 0.28 -0.6 1,359,226 + RR42_RS06190 0.28 -0.7 1,359,489 + RR42_RS06190 0.53 -1.9 1,359,489 + RR42_RS06190 0.53 -0.4 1,359,489 + RR42_RS06190 0.53 +1.5 1,359,489 + RR42_RS06190 0.53 -0.4 1,359,490 - RR42_RS06190 0.53 -0.5 1,359,490 - RR42_RS06190 0.53 +0.8 1,359,490 - RR42_RS06190 0.53 -0.3 1,359,490 - RR42_RS06190 0.53 +0.4 1,359,490 - RR42_RS06190 0.53 +1.8 1,359,491 + RR42_RS06190 0.53 +2.8 1,359,491 + RR42_RS06190 0.53 +0.2 1,359,491 + RR42_RS06190 0.53 -0.6 1,359,491 + RR42_RS06190 0.53 -0.0 1,359,491 + RR42_RS06190 0.53 -2.3 1,359,492 - RR42_RS06190 0.54 -0.2 1,359,492 - RR42_RS06190 0.54 -1.4 1,359,492 - RR42_RS06190 0.54 -0.7 1,359,492 - RR42_RS06190 0.54 -0.1 1,359,492 - RR42_RS06190 0.54 -0.4 1,359,492 - RR42_RS06190 0.54 -1.9
Or see this region's nucleotide sequence