Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06085 and RR42_RS06090 are separated by 21 nucleotides RR42_RS06090 and RR42_RS06095 are separated by 27 nucleotides
RR42_RS06085: RR42_RS06085 - peptidase, at 1,335,015 to 1,335,737
_RS06085
RR42_RS06090: RR42_RS06090 - single-stranded DNA exonuclease, at 1,335,759 to 1,337,456
_RS06090
RR42_RS06095: RR42_RS06095 - hypothetical protein, at 1,337,484 to 1,338,608
_RS06095
Position (kb)
1335
1336
1337
1338 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1334.834 kb on - strand at 1334.912 kb on + strand at 1335.005 kb on + strand at 1335.006 kb on - strand at 1335.006 kb on - strand at 1335.006 kb on - strand at 1335.006 kb on - strand at 1335.009 kb on + strand at 1335.009 kb on + strand at 1335.010 kb on - strand at 1335.010 kb on - strand at 1335.010 kb on - strand at 1335.141 kb on + strand, within RR42_RS06085 at 1335.141 kb on + strand, within RR42_RS06085 at 1335.171 kb on + strand, within RR42_RS06085 at 1335.171 kb on + strand, within RR42_RS06085 at 1335.172 kb on - strand, within RR42_RS06085 at 1335.172 kb on - strand, within RR42_RS06085 at 1335.172 kb on - strand, within RR42_RS06085 at 1335.579 kb on + strand, within RR42_RS06085 at 1335.622 kb on + strand, within RR42_RS06085 at 1335.623 kb on - strand, within RR42_RS06085 at 1335.904 kb on - strand at 1336.321 kb on + strand, within RR42_RS06090 at 1336.395 kb on - strand, within RR42_RS06090 at 1336.560 kb on + strand, within RR42_RS06090 at 1336.870 kb on + strand, within RR42_RS06090 at 1336.870 kb on + strand, within RR42_RS06090 at 1336.871 kb on - strand, within RR42_RS06090 at 1337.146 kb on + strand, within RR42_RS06090 at 1337.147 kb on - strand, within RR42_RS06090 at 1337.218 kb on + strand, within RR42_RS06090 at 1337.219 kb on - strand, within RR42_RS06090 at 1337.269 kb on + strand, within RR42_RS06090 at 1337.495 kb on - strand at 1337.495 kb on - strand at 1337.974 kb on + strand, within RR42_RS06095 at 1338.050 kb on + strand, within RR42_RS06095 at 1338.350 kb on - strand, within RR42_RS06095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,334,834 - +2.4 1,334,912 + +2.8 1,335,005 + +0.4 1,335,006 - +0.8 1,335,006 - -0.7 1,335,006 - +0.5 1,335,006 - +0.2 1,335,009 + +0.9 1,335,009 + +1.4 1,335,010 - -0.1 1,335,010 - +0.6 1,335,010 - +0.3 1,335,141 + RR42_RS06085 0.17 -0.6 1,335,141 + RR42_RS06085 0.17 -1.9 1,335,171 + RR42_RS06085 0.22 -1.0 1,335,171 + RR42_RS06085 0.22 -0.5 1,335,172 - RR42_RS06085 0.22 -0.4 1,335,172 - RR42_RS06085 0.22 -0.3 1,335,172 - RR42_RS06085 0.22 -0.1 1,335,579 + RR42_RS06085 0.78 +4.3 1,335,622 + RR42_RS06085 0.84 -0.5 1,335,623 - RR42_RS06085 0.84 -1.6 1,335,904 - +0.5 1,336,321 + RR42_RS06090 0.33 -1.0 1,336,395 - RR42_RS06090 0.37 +0.5 1,336,560 + RR42_RS06090 0.47 +1.1 1,336,870 + RR42_RS06090 0.65 +1.1 1,336,870 + RR42_RS06090 0.65 +0.1 1,336,871 - RR42_RS06090 0.65 +0.8 1,337,146 + RR42_RS06090 0.82 +0.0 1,337,147 - RR42_RS06090 0.82 +1.1 1,337,218 + RR42_RS06090 0.86 +0.3 1,337,219 - RR42_RS06090 0.86 +0.7 1,337,269 + RR42_RS06090 0.89 +0.4 1,337,495 - +0.1 1,337,495 - +1.2 1,337,974 + RR42_RS06095 0.44 +2.8 1,338,050 + RR42_RS06095 0.50 +0.8 1,338,350 - RR42_RS06095 0.77 +3.6
Or see this region's nucleotide sequence