Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05595 and RR42_RS05600 are separated by 13 nucleotides RR42_RS05600 and RR42_RS05605 are separated by 145 nucleotides RR42_RS05605 and RR42_RS05610 are separated by 83 nucleotides
RR42_RS05595: RR42_RS05595 - acyl-CoA dehydrogenase, at 1,231,906 to 1,233,108
_RS05595
RR42_RS05600: RR42_RS05600 - acyl-CoA dehydrogenase, at 1,233,122 to 1,234,240
_RS05600
RR42_RS05605: RR42_RS05605 - dehydratase, at 1,234,386 to 1,234,835
_RS05605
RR42_RS05610: RR42_RS05610 - dehydratase, at 1,234,919 to 1,235,398
_RS05610
Position (kb) 1233
1234
1235 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1232.130 kb on + strand, within RR42_RS05595 at 1232.216 kb on - strand, within RR42_RS05595 at 1232.239 kb on + strand, within RR42_RS05595 at 1232.240 kb on - strand, within RR42_RS05595 at 1232.243 kb on - strand, within RR42_RS05595 at 1232.362 kb on + strand, within RR42_RS05595 at 1232.363 kb on - strand, within RR42_RS05595 at 1232.363 kb on - strand, within RR42_RS05595 at 1232.363 kb on - strand, within RR42_RS05595 at 1232.627 kb on - strand, within RR42_RS05595 at 1232.956 kb on + strand, within RR42_RS05595 at 1233.022 kb on + strand at 1233.023 kb on - strand at 1233.023 kb on - strand at 1233.050 kb on - strand at 1233.686 kb on - strand, within RR42_RS05600 at 1233.686 kb on - strand, within RR42_RS05600 at 1233.692 kb on + strand, within RR42_RS05600 at 1233.724 kb on + strand, within RR42_RS05600 at 1233.725 kb on - strand, within RR42_RS05600 at 1233.872 kb on + strand, within RR42_RS05600 at 1233.872 kb on + strand, within RR42_RS05600 at 1234.356 kb on - strand at 1234.398 kb on + strand at 1234.485 kb on + strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.536 kb on + strand, within RR42_RS05605 at 1234.536 kb on + strand, within RR42_RS05605 at 1234.537 kb on - strand, within RR42_RS05605 at 1234.537 kb on - strand, within RR42_RS05605 at 1234.668 kb on + strand, within RR42_RS05605 at 1234.668 kb on + strand, within RR42_RS05605 at 1234.815 kb on + strand at 1234.815 kb on + strand at 1234.815 kb on + strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.884 kb on + strand at 1234.884 kb on + strand at 1234.884 kb on + strand at 1234.885 kb on - strand at 1234.885 kb on - strand at 1234.893 kb on + strand at 1234.893 kb on + strand at 1234.894 kb on - strand at 1234.894 kb on - strand at 1234.902 kb on + strand at 1234.902 kb on + strand at 1234.925 kb on - strand at 1235.187 kb on - strand, within RR42_RS05610 at 1235.209 kb on + strand, within RR42_RS05610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,232,130 + RR42_RS05595 0.19 +1.6 1,232,216 - RR42_RS05595 0.26 -0.5 1,232,239 + RR42_RS05595 0.28 +0.1 1,232,240 - RR42_RS05595 0.28 +1.8 1,232,243 - RR42_RS05595 0.28 +0.3 1,232,362 + RR42_RS05595 0.38 +1.0 1,232,363 - RR42_RS05595 0.38 -1.3 1,232,363 - RR42_RS05595 0.38 +0.7 1,232,363 - RR42_RS05595 0.38 +1.2 1,232,627 - RR42_RS05595 0.60 -0.2 1,232,956 + RR42_RS05595 0.87 +0.1 1,233,022 + +0.6 1,233,023 - -0.5 1,233,023 - -0.7 1,233,050 - +1.9 1,233,686 - RR42_RS05600 0.50 +0.2 1,233,686 - RR42_RS05600 0.50 +1.4 1,233,692 + RR42_RS05600 0.51 +1.2 1,233,724 + RR42_RS05600 0.54 -1.5 1,233,725 - RR42_RS05600 0.54 +1.4 1,233,872 + RR42_RS05600 0.67 +3.1 1,233,872 + RR42_RS05600 0.67 -2.0 1,234,356 - -1.1 1,234,398 + +0.9 1,234,485 + RR42_RS05605 0.22 -0.1 1,234,486 - RR42_RS05605 0.22 -2.4 1,234,486 - RR42_RS05605 0.22 -0.2 1,234,486 - RR42_RS05605 0.22 -1.4 1,234,486 - RR42_RS05605 0.22 +0.1 1,234,536 + RR42_RS05605 0.33 +2.2 1,234,536 + RR42_RS05605 0.33 +2.0 1,234,537 - RR42_RS05605 0.34 -0.9 1,234,537 - RR42_RS05605 0.34 -0.3 1,234,668 + RR42_RS05605 0.63 +1.5 1,234,668 + RR42_RS05605 0.63 -2.5 1,234,815 + +0.8 1,234,815 + -0.1 1,234,815 + -1.6 1,234,816 - +2.2 1,234,816 - +2.8 1,234,816 - -2.4 1,234,816 - -2.3 1,234,816 - -0.1 1,234,884 + +0.9 1,234,884 + +0.2 1,234,884 + -1.0 1,234,885 - +0.8 1,234,885 - -0.9 1,234,893 + -1.6 1,234,893 + +0.5 1,234,894 - +0.1 1,234,894 - -0.9 1,234,902 + -0.2 1,234,902 + -1.7 1,234,925 - +1.8 1,235,187 - RR42_RS05610 0.56 -0.9 1,235,209 + RR42_RS05610 0.60 +0.8
Or see this region's nucleotide sequence