Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05265

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05260 and RR42_RS05265 are separated by 150 nucleotidesRR42_RS05265 and RR42_RS05270 are separated by 313 nucleotidesRR42_RS05270 and RR42_RS05275 are separated by 31 nucleotides RR42_RS05260: RR42_RS05260 - purine permease, at 1,160,904 to 1,162,310 _RS05260 RR42_RS05265: RR42_RS05265 - GntR family transcriptional regulator, at 1,162,461 to 1,163,204 _RS05265 RR42_RS05270: RR42_RS05270 - 5-hydroxyisourate hydrolase, at 1,163,518 to 1,163,871 _RS05270 RR42_RS05275: RR42_RS05275 - membrane protein, at 1,163,903 to 1,165,108 _RS05275 Position (kb) 1162 1163 1164Strain fitness (log2 ratio) -2 -1 0 1 2at 1161.694 kb on + strand, within RR42_RS05260at 1161.695 kb on - strand, within RR42_RS05260at 1161.760 kb on + strand, within RR42_RS05260at 1161.974 kb on - strand, within RR42_RS05260at 1162.210 kb on + strandat 1162.210 kb on + strandat 1162.210 kb on + strandat 1162.210 kb on + strandat 1162.210 kb on + strandat 1162.211 kb on - strandat 1162.211 kb on - strandat 1162.211 kb on - strandat 1162.211 kb on - strandat 1162.211 kb on - strandat 1162.304 kb on + strandat 1162.305 kb on - strandat 1162.305 kb on - strandat 1162.371 kb on - strandat 1162.371 kb on - strandat 1162.371 kb on - strandat 1162.462 kb on + strandat 1162.462 kb on + strandat 1162.462 kb on + strandat 1162.462 kb on + strandat 1162.463 kb on - strandat 1162.463 kb on - strandat 1162.463 kb on - strandat 1162.463 kb on - strandat 1163.095 kb on + strand, within RR42_RS05265at 1163.095 kb on + strand, within RR42_RS05265at 1163.095 kb on + strand, within RR42_RS05265at 1163.096 kb on - strand, within RR42_RS05265at 1163.107 kb on + strand, within RR42_RS05265at 1163.107 kb on + strand, within RR42_RS05265at 1163.107 kb on + strand, within RR42_RS05265at 1163.108 kb on - strand, within RR42_RS05265at 1163.108 kb on - strand, within RR42_RS05265at 1163.108 kb on - strand, within RR42_RS05265at 1163.108 kb on - strand, within RR42_RS05265at 1163.218 kb on + strandat 1163.219 kb on - strandat 1163.313 kb on + strandat 1163.431 kb on + strandat 1163.432 kb on - strandat 1163.432 kb on - strandat 1163.467 kb on + strandat 1163.468 kb on - strandat 1163.540 kb on + strandat 1163.540 kb on + strandat 1163.698 kb on + strand, within RR42_RS05270at 1163.699 kb on - strand, within RR42_RS05270at 1163.699 kb on - strand, within RR42_RS05270at 1163.699 kb on - strand, within RR42_RS05270at 1163.815 kb on + strand, within RR42_RS05270at 1163.857 kb on + strandat 1163.858 kb on - strandat 1163.858 kb on - strandat 1163.869 kb on + strandat 1163.869 kb on + strandat 1163.870 kb on - strandat 1163.912 kb on + strandat 1163.912 kb on + strandat 1163.912 kb on + strandat 1163.913 kb on - strandat 1163.913 kb on - strandat 1163.915 kb on - strandat 1163.915 kb on - strandat 1163.915 kb on - strandat 1163.991 kb on - strandat 1164.092 kb on + strand, within RR42_RS05275at 1164.107 kb on + strand, within RR42_RS05275at 1164.153 kb on - strand, within RR42_RS05275at 1164.164 kb on + strand, within RR42_RS05275at 1164.164 kb on + strand, within RR42_RS05275at 1164.165 kb on - strand, within RR42_RS05275at 1164.165 kb on - strand, within RR42_RS05275

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,161,694 + RR42_RS05260 0.56 -1.3
1,161,695 - RR42_RS05260 0.56 +1.8
1,161,760 + RR42_RS05260 0.61 +1.8
1,161,974 - RR42_RS05260 0.76 -0.6
1,162,210 + -0.5
1,162,210 + +1.6
1,162,210 + -1.1
1,162,210 + -1.3
1,162,210 + +0.2
1,162,211 - -1.3
1,162,211 - +1.8
1,162,211 - +0.2
1,162,211 - +0.0
1,162,211 - -0.1
1,162,304 + +0.5
1,162,305 - +1.2
1,162,305 - +0.2
1,162,371 - -0.3
1,162,371 - +2.0
1,162,371 - -2.0
1,162,462 + -1.0
1,162,462 + -0.6
1,162,462 + +1.8
1,162,462 + -0.5
1,162,463 - +0.3
1,162,463 - +0.7
1,162,463 - +0.5
1,162,463 - +1.4
1,163,095 + RR42_RS05265 0.85 +0.1
1,163,095 + RR42_RS05265 0.85 -1.4
1,163,095 + RR42_RS05265 0.85 -2.2
1,163,096 - RR42_RS05265 0.85 -0.3
1,163,107 + RR42_RS05265 0.87 +0.4
1,163,107 + RR42_RS05265 0.87 -2.0
1,163,107 + RR42_RS05265 0.87 +1.7
1,163,108 - RR42_RS05265 0.87 -1.8
1,163,108 - RR42_RS05265 0.87 -1.4
1,163,108 - RR42_RS05265 0.87 +0.5
1,163,108 - RR42_RS05265 0.87 +0.4
1,163,218 + -0.8
1,163,219 - +0.0
1,163,313 + +0.1
1,163,431 + -2.4
1,163,432 - -1.3
1,163,432 - +0.7
1,163,467 + +0.3
1,163,468 - -1.6
1,163,540 + +1.0
1,163,540 + +1.3
1,163,698 + RR42_RS05270 0.51 +0.4
1,163,699 - RR42_RS05270 0.51 -0.2
1,163,699 - RR42_RS05270 0.51 -0.3
1,163,699 - RR42_RS05270 0.51 -0.3
1,163,815 + RR42_RS05270 0.84 -0.7
1,163,857 + +1.4
1,163,858 - +0.0
1,163,858 - +1.7
1,163,869 + -2.2
1,163,869 + +0.2
1,163,870 - -0.4
1,163,912 + +0.8
1,163,912 + -0.5
1,163,912 + +0.7
1,163,913 - +0.7
1,163,913 - +0.7
1,163,915 - +0.2
1,163,915 - +1.8
1,163,915 - +0.5
1,163,991 - +1.0
1,164,092 + RR42_RS05275 0.16 +1.4
1,164,107 + RR42_RS05275 0.17 +0.5
1,164,153 - RR42_RS05275 0.21 -0.2
1,164,164 + RR42_RS05275 0.22 -0.3
1,164,164 + RR42_RS05275 0.22 -0.2
1,164,165 - RR42_RS05275 0.22 -1.5
1,164,165 - RR42_RS05275 0.22 +0.6

Or see this region's nucleotide sequence