Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05260 and RR42_RS05265 are separated by 150 nucleotides RR42_RS05265 and RR42_RS05270 are separated by 313 nucleotides RR42_RS05270 and RR42_RS05275 are separated by 31 nucleotides
RR42_RS05260: RR42_RS05260 - purine permease, at 1,160,904 to 1,162,310
_RS05260
RR42_RS05265: RR42_RS05265 - GntR family transcriptional regulator, at 1,162,461 to 1,163,204
_RS05265
RR42_RS05270: RR42_RS05270 - 5-hydroxyisourate hydrolase, at 1,163,518 to 1,163,871
_RS05270
RR42_RS05275: RR42_RS05275 - membrane protein, at 1,163,903 to 1,165,108
_RS05275
Position (kb)
1162
1163
1164 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1161.694 kb on + strand, within RR42_RS05260 at 1161.695 kb on - strand, within RR42_RS05260 at 1161.760 kb on + strand, within RR42_RS05260 at 1161.974 kb on - strand, within RR42_RS05260 at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.210 kb on + strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.211 kb on - strand at 1162.304 kb on + strand at 1162.305 kb on - strand at 1162.305 kb on - strand at 1162.371 kb on - strand at 1162.371 kb on - strand at 1162.371 kb on - strand at 1162.462 kb on + strand at 1162.462 kb on + strand at 1162.462 kb on + strand at 1162.462 kb on + strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1163.095 kb on + strand, within RR42_RS05265 at 1163.095 kb on + strand, within RR42_RS05265 at 1163.095 kb on + strand, within RR42_RS05265 at 1163.096 kb on - strand, within RR42_RS05265 at 1163.107 kb on + strand, within RR42_RS05265 at 1163.107 kb on + strand, within RR42_RS05265 at 1163.107 kb on + strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.108 kb on - strand, within RR42_RS05265 at 1163.218 kb on + strand at 1163.219 kb on - strand at 1163.313 kb on + strand at 1163.431 kb on + strand at 1163.432 kb on - strand at 1163.432 kb on - strand at 1163.467 kb on + strand at 1163.468 kb on - strand at 1163.540 kb on + strand at 1163.540 kb on + strand at 1163.698 kb on + strand, within RR42_RS05270 at 1163.699 kb on - strand, within RR42_RS05270 at 1163.699 kb on - strand, within RR42_RS05270 at 1163.699 kb on - strand, within RR42_RS05270 at 1163.815 kb on + strand, within RR42_RS05270 at 1163.857 kb on + strand at 1163.858 kb on - strand at 1163.858 kb on - strand at 1163.869 kb on + strand at 1163.869 kb on + strand at 1163.870 kb on - strand at 1163.912 kb on + strand at 1163.912 kb on + strand at 1163.912 kb on + strand at 1163.913 kb on - strand at 1163.913 kb on - strand at 1163.915 kb on - strand at 1163.915 kb on - strand at 1163.915 kb on - strand at 1163.991 kb on - strand at 1164.092 kb on + strand, within RR42_RS05275 at 1164.107 kb on + strand, within RR42_RS05275 at 1164.153 kb on - strand, within RR42_RS05275 at 1164.164 kb on + strand, within RR42_RS05275 at 1164.164 kb on + strand, within RR42_RS05275 at 1164.165 kb on - strand, within RR42_RS05275 at 1164.165 kb on - strand, within RR42_RS05275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,161,694 + RR42_RS05260 0.56 -1.3 1,161,695 - RR42_RS05260 0.56 +1.8 1,161,760 + RR42_RS05260 0.61 +1.8 1,161,974 - RR42_RS05260 0.76 -0.6 1,162,210 + -0.5 1,162,210 + +1.6 1,162,210 + -1.1 1,162,210 + -1.3 1,162,210 + +0.2 1,162,211 - -1.3 1,162,211 - +1.8 1,162,211 - +0.2 1,162,211 - +0.0 1,162,211 - -0.1 1,162,304 + +0.5 1,162,305 - +1.2 1,162,305 - +0.2 1,162,371 - -0.3 1,162,371 - +2.0 1,162,371 - -2.0 1,162,462 + -1.0 1,162,462 + -0.6 1,162,462 + +1.8 1,162,462 + -0.5 1,162,463 - +0.3 1,162,463 - +0.7 1,162,463 - +0.5 1,162,463 - +1.4 1,163,095 + RR42_RS05265 0.85 +0.1 1,163,095 + RR42_RS05265 0.85 -1.4 1,163,095 + RR42_RS05265 0.85 -2.2 1,163,096 - RR42_RS05265 0.85 -0.3 1,163,107 + RR42_RS05265 0.87 +0.4 1,163,107 + RR42_RS05265 0.87 -2.0 1,163,107 + RR42_RS05265 0.87 +1.7 1,163,108 - RR42_RS05265 0.87 -1.8 1,163,108 - RR42_RS05265 0.87 -1.4 1,163,108 - RR42_RS05265 0.87 +0.5 1,163,108 - RR42_RS05265 0.87 +0.4 1,163,218 + -0.8 1,163,219 - +0.0 1,163,313 + +0.1 1,163,431 + -2.4 1,163,432 - -1.3 1,163,432 - +0.7 1,163,467 + +0.3 1,163,468 - -1.6 1,163,540 + +1.0 1,163,540 + +1.3 1,163,698 + RR42_RS05270 0.51 +0.4 1,163,699 - RR42_RS05270 0.51 -0.2 1,163,699 - RR42_RS05270 0.51 -0.3 1,163,699 - RR42_RS05270 0.51 -0.3 1,163,815 + RR42_RS05270 0.84 -0.7 1,163,857 + +1.4 1,163,858 - +0.0 1,163,858 - +1.7 1,163,869 + -2.2 1,163,869 + +0.2 1,163,870 - -0.4 1,163,912 + +0.8 1,163,912 + -0.5 1,163,912 + +0.7 1,163,913 - +0.7 1,163,913 - +0.7 1,163,915 - +0.2 1,163,915 - +1.8 1,163,915 - +0.5 1,163,991 - +1.0 1,164,092 + RR42_RS05275 0.16 +1.4 1,164,107 + RR42_RS05275 0.17 +0.5 1,164,153 - RR42_RS05275 0.21 -0.2 1,164,164 + RR42_RS05275 0.22 -0.3 1,164,164 + RR42_RS05275 0.22 -0.2 1,164,165 - RR42_RS05275 0.22 -1.5 1,164,165 - RR42_RS05275 0.22 +0.6
Or see this region's nucleotide sequence