Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05245 and RR42_RS05250 are separated by 276 nucleotides RR42_RS05250 and RR42_RS05255 are separated by 8 nucleotides RR42_RS05255 and RR42_RS05260 are separated by 48 nucleotides
RR42_RS05245: RR42_RS05245 - porin, at 1,158,016 to 1,159,101
_RS05245
RR42_RS05250: RR42_RS05250 - OHCU decarboxylase, at 1,159,378 to 1,159,896
_RS05250
RR42_RS05255: RR42_RS05255 - allantoinase, at 1,159,905 to 1,160,855
_RS05255
RR42_RS05260: RR42_RS05260 - purine permease, at 1,160,904 to 1,162,310
_RS05260
Position (kb)
1159
1160
1161 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1158.914 kb on + strand, within RR42_RS05245 at 1158.947 kb on + strand, within RR42_RS05245 at 1158.947 kb on + strand, within RR42_RS05245 at 1158.947 kb on + strand, within RR42_RS05245 at 1159.008 kb on - strand at 1159.096 kb on - strand at 1159.141 kb on + strand at 1159.163 kb on - strand at 1159.165 kb on + strand at 1159.165 kb on + strand at 1159.299 kb on + strand at 1159.321 kb on + strand at 1159.390 kb on + strand at 1159.390 kb on + strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.391 kb on - strand at 1159.554 kb on - strand, within RR42_RS05250 at 1159.820 kb on + strand, within RR42_RS05250 at 1159.820 kb on + strand, within RR42_RS05250 at 1159.820 kb on + strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.821 kb on - strand, within RR42_RS05250 at 1159.883 kb on + strand at 1159.883 kb on + strand at 1159.884 kb on - strand at 1160.116 kb on + strand, within RR42_RS05255 at 1160.117 kb on - strand, within RR42_RS05255 at 1160.214 kb on - strand, within RR42_RS05255 at 1160.214 kb on - strand, within RR42_RS05255 at 1160.214 kb on - strand, within RR42_RS05255 at 1160.216 kb on - strand, within RR42_RS05255 at 1160.291 kb on - strand, within RR42_RS05255 at 1160.294 kb on - strand, within RR42_RS05255 at 1160.306 kb on - strand, within RR42_RS05255 at 1160.306 kb on - strand, within RR42_RS05255 at 1160.306 kb on - strand, within RR42_RS05255 at 1160.459 kb on - strand, within RR42_RS05255 at 1160.459 kb on - strand, within RR42_RS05255 at 1160.632 kb on + strand, within RR42_RS05255 at 1160.633 kb on - strand, within RR42_RS05255 at 1160.750 kb on - strand, within RR42_RS05255 at 1160.750 kb on - strand, within RR42_RS05255 at 1160.839 kb on + strand at 1160.905 kb on + strand at 1160.906 kb on - strand at 1160.906 kb on - strand at 1161.053 kb on - strand, within RR42_RS05260 at 1161.053 kb on - strand, within RR42_RS05260 at 1161.358 kb on + strand, within RR42_RS05260 at 1161.359 kb on - strand, within RR42_RS05260 at 1161.694 kb on + strand, within RR42_RS05260 at 1161.695 kb on - strand, within RR42_RS05260 at 1161.760 kb on + strand, within RR42_RS05260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 1,158,914 + RR42_RS05245 0.83 +1.8 1,158,947 + RR42_RS05245 0.86 -2.0 1,158,947 + RR42_RS05245 0.86 -2.1 1,158,947 + RR42_RS05245 0.86 -0.5 1,159,008 - +0.5 1,159,096 - -0.8 1,159,141 + -1.3 1,159,163 - +1.1 1,159,165 + -1.2 1,159,165 + +2.8 1,159,299 + +0.2 1,159,321 + +0.4 1,159,390 + +0.1 1,159,390 + +0.2 1,159,391 - +0.4 1,159,391 - -0.6 1,159,391 - -1.4 1,159,391 - -0.9 1,159,391 - -2.2 1,159,554 - RR42_RS05250 0.34 +0.0 1,159,820 + RR42_RS05250 0.85 -0.2 1,159,820 + RR42_RS05250 0.85 +0.3 1,159,820 + RR42_RS05250 0.85 +0.6 1,159,821 - RR42_RS05250 0.85 +0.4 1,159,821 - RR42_RS05250 0.85 -0.3 1,159,821 - RR42_RS05250 0.85 +0.1 1,159,821 - RR42_RS05250 0.85 +0.8 1,159,821 - RR42_RS05250 0.85 -1.5 1,159,821 - RR42_RS05250 0.85 +2.7 1,159,821 - RR42_RS05250 0.85 +0.3 1,159,883 + +0.8 1,159,883 + +0.6 1,159,884 - +0.8 1,160,116 + RR42_RS05255 0.22 -0.4 1,160,117 - RR42_RS05255 0.22 -1.3 1,160,214 - RR42_RS05255 0.32 -1.3 1,160,214 - RR42_RS05255 0.32 -1.1 1,160,214 - RR42_RS05255 0.32 +0.7 1,160,216 - RR42_RS05255 0.33 +0.3 1,160,291 - RR42_RS05255 0.41 +0.4 1,160,294 - RR42_RS05255 0.41 +0.8 1,160,306 - RR42_RS05255 0.42 +1.2 1,160,306 - RR42_RS05255 0.42 -0.2 1,160,306 - RR42_RS05255 0.42 +0.2 1,160,459 - RR42_RS05255 0.58 -0.1 1,160,459 - RR42_RS05255 0.58 +0.6 1,160,632 + RR42_RS05255 0.76 +0.2 1,160,633 - RR42_RS05255 0.77 +0.8 1,160,750 - RR42_RS05255 0.89 -0.5 1,160,750 - RR42_RS05255 0.89 -1.5 1,160,839 + -0.7 1,160,905 + +0.4 1,160,906 - -2.4 1,160,906 - -0.8 1,161,053 - RR42_RS05260 0.11 -1.1 1,161,053 - RR42_RS05260 0.11 +0.4 1,161,358 + RR42_RS05260 0.32 +0.4 1,161,359 - RR42_RS05260 0.32 +1.0 1,161,694 + RR42_RS05260 0.56 -1.3 1,161,695 - RR42_RS05260 0.56 +1.8 1,161,760 + RR42_RS05260 0.61 +1.8
Or see this region's nucleotide sequence