Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS04655

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS04650 and RR42_RS04655 are separated by 98 nucleotidesRR42_RS04655 and RR42_RS04660 overlap by 53 nucleotidesRR42_RS04660 and RR42_RS04665 are separated by 11 nucleotides RR42_RS04650: RR42_RS04650 - DNA polymerase III subunit alpha, at 1,034,185 to 1,037,697 _RS04650 RR42_RS04655: RR42_RS04655 - glycosyl transferase, at 1,037,796 to 1,039,052 _RS04655 RR42_RS04660: RR42_RS04660 - glycosyl transferase family 2, at 1,039,000 to 1,039,767 _RS04660 RR42_RS04665: RR42_RS04665 - cell shape-determining protein, at 1,039,779 to 1,041,032 _RS04665 Position (kb) 1037 1038 1039 1040Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1037.689 kb on + strandat 1037.689 kb on + strandat 1037.689 kb on + strandat 1037.690 kb on - strandat 1037.782 kb on + strandat 1037.782 kb on + strandat 1037.782 kb on + strandat 1037.782 kb on + strandat 1037.783 kb on - strandat 1037.783 kb on - strandat 1037.982 kb on - strand, within RR42_RS04655at 1038.058 kb on + strand, within RR42_RS04655at 1038.058 kb on + strand, within RR42_RS04655at 1038.058 kb on + strand, within RR42_RS04655at 1038.058 kb on + strand, within RR42_RS04655at 1038.058 kb on + strand, within RR42_RS04655at 1038.059 kb on - strand, within RR42_RS04655at 1038.059 kb on - strand, within RR42_RS04655at 1038.060 kb on + strand, within RR42_RS04655at 1038.060 kb on + strand, within RR42_RS04655at 1038.060 kb on + strand, within RR42_RS04655at 1038.060 kb on + strand, within RR42_RS04655at 1038.061 kb on - strand, within RR42_RS04655at 1038.061 kb on - strand, within RR42_RS04655at 1038.061 kb on - strand, within RR42_RS04655at 1038.132 kb on + strand, within RR42_RS04655at 1038.132 kb on + strand, within RR42_RS04655at 1038.150 kb on + strand, within RR42_RS04655at 1038.285 kb on + strand, within RR42_RS04655at 1038.285 kb on + strand, within RR42_RS04655at 1038.285 kb on + strand, within RR42_RS04655at 1038.285 kb on + strand, within RR42_RS04655at 1038.286 kb on - strand, within RR42_RS04655at 1038.286 kb on - strand, within RR42_RS04655at 1038.286 kb on - strand, within RR42_RS04655at 1038.372 kb on + strand, within RR42_RS04655at 1038.373 kb on - strand, within RR42_RS04655at 1038.373 kb on - strand, within RR42_RS04655at 1038.441 kb on + strand, within RR42_RS04655at 1038.441 kb on + strand, within RR42_RS04655at 1038.441 kb on + strand, within RR42_RS04655at 1038.441 kb on + strand, within RR42_RS04655at 1038.442 kb on - strand, within RR42_RS04655at 1038.442 kb on - strand, within RR42_RS04655at 1038.442 kb on - strand, within RR42_RS04655at 1038.442 kb on - strand, within RR42_RS04655at 1038.442 kb on - strand, within RR42_RS04655at 1038.649 kb on - strand, within RR42_RS04655at 1039.778 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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1,037,689 + -1.8
1,037,689 + -2.4
1,037,689 + +0.4
1,037,690 - -0.1
1,037,782 + +0.0
1,037,782 + -0.8
1,037,782 + +2.0
1,037,782 + -0.7
1,037,783 - +0.5
1,037,783 - +0.1
1,037,982 - RR42_RS04655 0.15 +0.8
1,038,058 + RR42_RS04655 0.21 -0.4
1,038,058 + RR42_RS04655 0.21 +2.1
1,038,058 + RR42_RS04655 0.21 +2.2
1,038,058 + RR42_RS04655 0.21 -0.7
1,038,058 + RR42_RS04655 0.21 +2.3
1,038,059 - RR42_RS04655 0.21 -0.6
1,038,059 - RR42_RS04655 0.21 -1.7
1,038,060 + RR42_RS04655 0.21 +0.9
1,038,060 + RR42_RS04655 0.21 -0.6
1,038,060 + RR42_RS04655 0.21 +0.4
1,038,060 + RR42_RS04655 0.21 +0.7
1,038,061 - RR42_RS04655 0.21 +1.0
1,038,061 - RR42_RS04655 0.21 +0.9
1,038,061 - RR42_RS04655 0.21 -0.6
1,038,132 + RR42_RS04655 0.27 +0.4
1,038,132 + RR42_RS04655 0.27 +1.3
1,038,150 + RR42_RS04655 0.28 -0.6
1,038,285 + RR42_RS04655 0.39 +0.7
1,038,285 + RR42_RS04655 0.39 +0.2
1,038,285 + RR42_RS04655 0.39 +0.8
1,038,285 + RR42_RS04655 0.39 +1.2
1,038,286 - RR42_RS04655 0.39 +3.7
1,038,286 - RR42_RS04655 0.39 +0.2
1,038,286 - RR42_RS04655 0.39 -0.6
1,038,372 + RR42_RS04655 0.46 -1.2
1,038,373 - RR42_RS04655 0.46 +0.3
1,038,373 - RR42_RS04655 0.46 -0.4
1,038,441 + RR42_RS04655 0.51 +0.9
1,038,441 + RR42_RS04655 0.51 -1.1
1,038,441 + RR42_RS04655 0.51 +0.3
1,038,441 + RR42_RS04655 0.51 +0.2
1,038,442 - RR42_RS04655 0.51 +1.2
1,038,442 - RR42_RS04655 0.51 +1.4
1,038,442 - RR42_RS04655 0.51 +0.0
1,038,442 - RR42_RS04655 0.51 -0.4
1,038,442 - RR42_RS04655 0.51 -1.1
1,038,649 - RR42_RS04655 0.68 +1.0
1,039,778 + +0.9

Or see this region's nucleotide sequence