Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04425 and RR42_RS04430 are separated by 770 nucleotides RR42_RS04430 and RR42_RS04435 are separated by 2 nucleotides RR42_RS04435 and RR42_RS04440 are separated by 111 nucleotides
RR42_RS04425: RR42_RS04425 - hypothetical protein, at 988,014 to 988,877
_RS04425
RR42_RS04430: RR42_RS04430 - phosphoglycolate phosphatase, at 989,648 to 990,319
_RS04430
RR42_RS04435: RR42_RS04435 - 3-demethylubiquinone-9 3-methyltransferase, at 990,322 to 991,071
_RS04435
RR42_RS04440: RR42_RS04440 - membrane protein, at 991,183 to 991,842
_RS04440
Position (kb) 989
990
991 Strain fitness (log2 ratio) -2
-1
0
1
2 at 988.688 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.690 kb on + strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.691 kb on - strand, within RR42_RS04425 at 988.881 kb on + strand at 988.881 kb on + strand at 988.882 kb on - strand at 988.951 kb on + strand at 988.963 kb on - strand at 988.963 kb on - strand at 988.963 kb on - strand at 989.078 kb on - strand at 989.078 kb on - strand at 989.126 kb on - strand at 989.126 kb on - strand at 989.149 kb on + strand at 989.149 kb on + strand at 989.149 kb on + strand at 989.149 kb on + strand at 989.150 kb on - strand at 989.270 kb on + strand at 989.271 kb on - strand at 989.313 kb on + strand at 989.369 kb on + strand at 989.369 kb on + strand at 989.369 kb on + strand at 989.369 kb on + strand at 989.370 kb on - strand at 989.370 kb on - strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.373 kb on + strand at 989.374 kb on - strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.377 kb on + strand at 989.378 kb on - strand at 989.424 kb on + strand at 989.424 kb on + strand at 989.425 kb on - strand at 989.488 kb on + strand at 989.501 kb on + strand at 989.501 kb on + strand at 989.501 kb on + strand at 989.553 kb on - strand at 989.568 kb on - strand at 989.599 kb on - strand at 989.621 kb on + strand at 989.621 kb on + strand at 989.621 kb on + strand at 989.706 kb on + strand at 989.755 kb on - strand, within RR42_RS04430 at 989.807 kb on + strand, within RR42_RS04430 at 989.807 kb on + strand, within RR42_RS04430 at 989.808 kb on - strand, within RR42_RS04430 at 989.808 kb on - strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.823 kb on + strand, within RR42_RS04430 at 989.824 kb on - strand, within RR42_RS04430 at 989.996 kb on - strand, within RR42_RS04430 at 989.996 kb on - strand, within RR42_RS04430 at 990.061 kb on + strand, within RR42_RS04430 at 990.061 kb on + strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.062 kb on - strand, within RR42_RS04430 at 990.084 kb on + strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.085 kb on - strand, within RR42_RS04430 at 990.088 kb on - strand, within RR42_RS04430 at 990.088 kb on - strand, within RR42_RS04430 at 990.309 kb on + strand at 990.310 kb on - strand at 990.310 kb on - strand at 991.128 kb on + strand at 991.129 kb on - strand at 991.184 kb on + strand at 991.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 988,688 + RR42_RS04425 0.78 -1.3 988,690 + RR42_RS04425 0.78 +0.5 988,690 + RR42_RS04425 0.78 -1.6 988,690 + RR42_RS04425 0.78 -0.8 988,690 + RR42_RS04425 0.78 +0.0 988,690 + RR42_RS04425 0.78 -1.1 988,691 - RR42_RS04425 0.78 -2.1 988,691 - RR42_RS04425 0.78 -1.6 988,691 - RR42_RS04425 0.78 +0.8 988,691 - RR42_RS04425 0.78 -0.5 988,691 - RR42_RS04425 0.78 +0.0 988,691 - RR42_RS04425 0.78 -0.6 988,691 - RR42_RS04425 0.78 +0.5 988,691 - RR42_RS04425 0.78 -0.1 988,881 + +0.7 988,881 + -0.4 988,882 - +0.2 988,951 + -0.9 988,963 - +1.8 988,963 - -1.4 988,963 - -0.2 989,078 - +0.2 989,078 - -1.6 989,126 - -0.8 989,126 - -0.3 989,149 + -1.0 989,149 + -0.2 989,149 + +0.2 989,149 + -0.6 989,150 - -0.7 989,270 + -0.9 989,271 - -0.1 989,313 + -0.3 989,369 + -0.9 989,369 + -0.7 989,369 + -0.1 989,369 + -0.4 989,370 - +1.0 989,370 - -0.1 989,373 + -0.9 989,373 + +1.2 989,373 + -0.6 989,373 + +0.8 989,373 + -1.6 989,374 - -0.4 989,377 + +0.3 989,377 + -0.4 989,377 + -0.8 989,377 + -0.5 989,377 + -0.6 989,378 - +0.2 989,424 + -1.4 989,424 + -1.2 989,425 - -1.2 989,488 + -2.4 989,501 + +1.0 989,501 + -0.7 989,501 + -2.0 989,553 - +1.8 989,568 - -0.5 989,599 - -1.8 989,621 + -0.2 989,621 + +0.0 989,621 + -0.2 989,706 + -0.2 989,755 - RR42_RS04430 0.16 +2.7 989,807 + RR42_RS04430 0.24 +0.5 989,807 + RR42_RS04430 0.24 +1.6 989,808 - RR42_RS04430 0.24 +1.4 989,808 - RR42_RS04430 0.24 +0.9 989,823 + RR42_RS04430 0.26 +0.2 989,823 + RR42_RS04430 0.26 +0.4 989,823 + RR42_RS04430 0.26 +0.6 989,823 + RR42_RS04430 0.26 -1.0 989,823 + RR42_RS04430 0.26 +0.2 989,824 - RR42_RS04430 0.26 +0.9 989,996 - RR42_RS04430 0.52 +0.8 989,996 - RR42_RS04430 0.52 +0.1 990,061 + RR42_RS04430 0.61 -0.5 990,061 + RR42_RS04430 0.61 -0.2 990,062 - RR42_RS04430 0.62 +0.8 990,062 - RR42_RS04430 0.62 +0.5 990,062 - RR42_RS04430 0.62 -0.6 990,062 - RR42_RS04430 0.62 +1.2 990,062 - RR42_RS04430 0.62 +1.8 990,084 + RR42_RS04430 0.65 -0.2 990,085 - RR42_RS04430 0.65 +0.0 990,085 - RR42_RS04430 0.65 +0.7 990,085 - RR42_RS04430 0.65 +0.0 990,085 - RR42_RS04430 0.65 -0.1 990,085 - RR42_RS04430 0.65 +1.9 990,085 - RR42_RS04430 0.65 +0.4 990,088 - RR42_RS04430 0.65 +0.4 990,088 - RR42_RS04430 0.65 +1.3 990,309 + +1.5 990,310 - -0.4 990,310 - -1.5 991,128 + -0.9 991,129 - -0.1 991,184 + +0.5 991,185 - +0.5
Or see this region's nucleotide sequence