Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04300 and RR42_RS04305 are separated by 95 nucleotides RR42_RS04305 and RR42_RS04310 are separated by 13 nucleotides RR42_RS04310 and RR42_RS04315 are separated by 133 nucleotides
RR42_RS04300: RR42_RS04300 - nitrogen regulatory protein P-II 1, at 956,043 to 956,381
_RS04300
RR42_RS04305: RR42_RS04305 - membrane protein, at 956,477 to 957,118
_RS04305
RR42_RS04310: RR42_RS04310 - DNA mismatch repair protein MutS, at 957,132 to 957,836
_RS04310
RR42_RS04315: RR42_RS04315 - thioredoxin reductase, at 957,970 to 958,917
_RS04315
Position (kb)
957
958 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 956.193 kb on + strand, within RR42_RS04300 at 956.193 kb on + strand, within RR42_RS04300 at 956.194 kb on - strand, within RR42_RS04300 at 956.194 kb on - strand, within RR42_RS04300 at 956.590 kb on + strand, within RR42_RS04305 at 956.591 kb on - strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.593 kb on - strand, within RR42_RS04305 at 956.593 kb on - strand, within RR42_RS04305 at 956.625 kb on + strand, within RR42_RS04305 at 956.626 kb on - strand, within RR42_RS04305 at 956.626 kb on - strand, within RR42_RS04305 at 956.843 kb on + strand, within RR42_RS04305 at 956.843 kb on + strand, within RR42_RS04305 at 956.889 kb on + strand, within RR42_RS04305 at 956.890 kb on - strand, within RR42_RS04305 at 956.890 kb on - strand, within RR42_RS04305 at 956.890 kb on - strand, within RR42_RS04305 at 956.890 kb on - strand, within RR42_RS04305 at 956.910 kb on + strand, within RR42_RS04305 at 956.910 kb on + strand, within RR42_RS04305 at 956.910 kb on + strand, within RR42_RS04305 at 956.911 kb on - strand, within RR42_RS04305 at 957.056 kb on + strand at 957.056 kb on + strand at 957.056 kb on + strand at 957.057 kb on - strand at 957.057 kb on - strand at 957.210 kb on + strand, within RR42_RS04310 at 957.210 kb on + strand, within RR42_RS04310 at 957.211 kb on - strand, within RR42_RS04310 at 957.645 kb on + strand, within RR42_RS04310 at 957.646 kb on - strand, within RR42_RS04310 at 957.865 kb on + strand at 957.866 kb on - strand at 957.866 kb on - strand at 957.867 kb on + strand at 957.867 kb on + strand at 957.868 kb on - strand at 957.868 kb on - strand at 957.971 kb on + strand at 957.972 kb on - strand at 957.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 956,193 + RR42_RS04300 0.44 +1.0 956,193 + RR42_RS04300 0.44 +1.2 956,194 - RR42_RS04300 0.45 +1.2 956,194 - RR42_RS04300 0.45 -0.3 956,590 + RR42_RS04305 0.18 -1.7 956,591 - RR42_RS04305 0.18 +2.1 956,592 + RR42_RS04305 0.18 -1.6 956,592 + RR42_RS04305 0.18 -2.4 956,592 + RR42_RS04305 0.18 +0.4 956,592 + RR42_RS04305 0.18 +0.0 956,592 + RR42_RS04305 0.18 -1.6 956,593 - RR42_RS04305 0.18 +3.4 956,593 - RR42_RS04305 0.18 -1.9 956,625 + RR42_RS04305 0.23 +0.2 956,626 - RR42_RS04305 0.23 -0.2 956,626 - RR42_RS04305 0.23 -0.1 956,843 + RR42_RS04305 0.57 -0.8 956,843 + RR42_RS04305 0.57 -1.7 956,889 + RR42_RS04305 0.64 -0.5 956,890 - RR42_RS04305 0.64 +1.3 956,890 - RR42_RS04305 0.64 +0.8 956,890 - RR42_RS04305 0.64 +0.6 956,890 - RR42_RS04305 0.64 -0.1 956,910 + RR42_RS04305 0.67 -0.3 956,910 + RR42_RS04305 0.67 +2.6 956,910 + RR42_RS04305 0.67 +0.6 956,911 - RR42_RS04305 0.68 -0.1 957,056 + -1.4 957,056 + +1.4 957,056 + +1.3 957,057 - -0.7 957,057 - +2.5 957,210 + RR42_RS04310 0.11 +0.2 957,210 + RR42_RS04310 0.11 -0.8 957,211 - RR42_RS04310 0.11 -0.8 957,645 + RR42_RS04310 0.73 +0.5 957,646 - RR42_RS04310 0.73 +0.8 957,865 + -1.6 957,866 - -0.2 957,866 - -0.4 957,867 + -0.1 957,867 + +0.5 957,868 - -1.6 957,868 - -0.9 957,971 + +0.1 957,972 - +1.2 957,972 - -2.0
Or see this region's nucleotide sequence