Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS02865

Experiment: R2A with Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS02855 and RR42_RS02860 are separated by 94 nucleotidesRR42_RS02860 and RR42_RS02865 are separated by 123 nucleotidesRR42_RS02865 and RR42_RS02870 are separated by 307 nucleotides RR42_RS02855: RR42_RS02855 - dimethylallyladenosine tRNA methylthiotransferase, at 636,191 to 637,543 _RS02855 RR42_RS02860: RR42_RS02860 - hypothetical protein, at 637,638 to 638,018 _RS02860 RR42_RS02865: RR42_RS02865 - chloride channel protein, at 638,142 to 639,830 _RS02865 RR42_RS02870: RR42_RS02870 - RNA helicase, at 640,138 to 641,826 _RS02870 Position (kb) 638 639 640Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 637.475 kb on - strandat 637.519 kb on - strandat 637.519 kb on - strandat 637.519 kb on - strandat 637.634 kb on - strandat 637.634 kb on - strandat 637.639 kb on + strandat 637.640 kb on - strandat 637.640 kb on - strandat 637.640 kb on - strandat 637.640 kb on - strandat 637.763 kb on + strand, within RR42_RS02860at 637.960 kb on - strand, within RR42_RS02860at 637.960 kb on - strand, within RR42_RS02860at 638.106 kb on + strandat 638.107 kb on - strandat 638.107 kb on - strandat 638.107 kb on - strandat 638.107 kb on - strandat 638.110 kb on - strandat 638.110 kb on - strandat 638.110 kb on - strandat 638.110 kb on - strandat 638.110 kb on - strandat 638.143 kb on + strandat 638.144 kb on - strandat 638.144 kb on - strandat 638.413 kb on + strand, within RR42_RS02865at 638.413 kb on + strand, within RR42_RS02865at 638.413 kb on + strand, within RR42_RS02865at 638.413 kb on + strand, within RR42_RS02865at 638.414 kb on - strand, within RR42_RS02865at 638.419 kb on + strand, within RR42_RS02865at 638.575 kb on + strand, within RR42_RS02865at 638.575 kb on + strand, within RR42_RS02865at 638.575 kb on + strand, within RR42_RS02865at 638.576 kb on - strand, within RR42_RS02865at 638.608 kb on + strand, within RR42_RS02865at 638.609 kb on - strand, within RR42_RS02865at 638.612 kb on - strand, within RR42_RS02865at 638.902 kb on - strand, within RR42_RS02865at 638.941 kb on + strand, within RR42_RS02865at 638.941 kb on + strand, within RR42_RS02865at 638.941 kb on + strand, within RR42_RS02865at 638.941 kb on + strand, within RR42_RS02865at 638.942 kb on - strand, within RR42_RS02865at 638.942 kb on - strand, within RR42_RS02865at 638.942 kb on - strand, within RR42_RS02865at 638.942 kb on - strand, within RR42_RS02865at 638.942 kb on - strand, within RR42_RS02865at 638.942 kb on - strand, within RR42_RS02865at 639.195 kb on + strand, within RR42_RS02865at 639.196 kb on - strand, within RR42_RS02865at 639.196 kb on - strand, within RR42_RS02865at 639.196 kb on - strand, within RR42_RS02865at 639.196 kb on - strand, within RR42_RS02865at 639.196 kb on - strand, within RR42_RS02865at 639.557 kb on + strand, within RR42_RS02865at 639.557 kb on + strand, within RR42_RS02865at 639.558 kb on - strand, within RR42_RS02865at 639.558 kb on - strand, within RR42_RS02865at 639.559 kb on + strand, within RR42_RS02865at 639.559 kb on + strand, within RR42_RS02865at 639.560 kb on - strand, within RR42_RS02865at 639.678 kb on + strandat 639.689 kb on + strandat 639.789 kb on - strandat 639.996 kb on - strandat 640.000 kb on + strandat 640.001 kb on - strandat 640.173 kb on + strandat 640.173 kb on + strandat 640.174 kb on - strandat 640.174 kb on - strandat 640.200 kb on + strandat 640.200 kb on + strandat 640.200 kb on + strandat 640.200 kb on + strandat 640.201 kb on - strandat 640.201 kb on - strandat 640.201 kb on - strandat 640.201 kb on - strandat 640.201 kb on - strandat 640.437 kb on + strand, within RR42_RS02870at 640.441 kb on + strand, within RR42_RS02870at 640.441 kb on + strand, within RR42_RS02870at 640.442 kb on - strand, within RR42_RS02870at 640.792 kb on - strand, within RR42_RS02870at 640.823 kb on - strand, within RR42_RS02870

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM
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637,475 - -0.7
637,519 - -2.7
637,519 - +0.4
637,519 - -1.5
637,634 - +0.5
637,634 - +0.4
637,639 + -1.6
637,640 - +1.2
637,640 - -0.0
637,640 - -2.7
637,640 - +0.2
637,763 + RR42_RS02860 0.33 -0.3
637,960 - RR42_RS02860 0.85 +0.1
637,960 - RR42_RS02860 0.85 -0.3
638,106 + -0.5
638,107 - +1.7
638,107 - -0.0
638,107 - +0.7
638,107 - +1.1
638,110 - +0.2
638,110 - -0.6
638,110 - +2.7
638,110 - -1.8
638,110 - -1.1
638,143 + -2.3
638,144 - +0.5
638,144 - +2.4
638,413 + RR42_RS02865 0.16 +3.1
638,413 + RR42_RS02865 0.16 +4.3
638,413 + RR42_RS02865 0.16 +1.6
638,413 + RR42_RS02865 0.16 -0.6
638,414 - RR42_RS02865 0.16 -1.1
638,419 + RR42_RS02865 0.16 +0.9
638,575 + RR42_RS02865 0.26 -0.8
638,575 + RR42_RS02865 0.26 -0.1
638,575 + RR42_RS02865 0.26 -0.4
638,576 - RR42_RS02865 0.26 -0.3
638,608 + RR42_RS02865 0.28 +1.9
638,609 - RR42_RS02865 0.28 +1.1
638,612 - RR42_RS02865 0.28 -1.0
638,902 - RR42_RS02865 0.45 +0.4
638,941 + RR42_RS02865 0.47 -1.4
638,941 + RR42_RS02865 0.47 +1.1
638,941 + RR42_RS02865 0.47 -0.9
638,941 + RR42_RS02865 0.47 -1.5
638,942 - RR42_RS02865 0.47 +1.1
638,942 - RR42_RS02865 0.47 +0.7
638,942 - RR42_RS02865 0.47 +0.4
638,942 - RR42_RS02865 0.47 -0.8
638,942 - RR42_RS02865 0.47 +3.0
638,942 - RR42_RS02865 0.47 +0.6
639,195 + RR42_RS02865 0.62 -0.1
639,196 - RR42_RS02865 0.62 +0.7
639,196 - RR42_RS02865 0.62 +0.0
639,196 - RR42_RS02865 0.62 -0.5
639,196 - RR42_RS02865 0.62 +0.4
639,196 - RR42_RS02865 0.62 -0.7
639,557 + RR42_RS02865 0.84 -0.9
639,557 + RR42_RS02865 0.84 +0.4
639,558 - RR42_RS02865 0.84 +0.5
639,558 - RR42_RS02865 0.84 +1.1
639,559 + RR42_RS02865 0.84 -0.9
639,559 + RR42_RS02865 0.84 +1.7
639,560 - RR42_RS02865 0.84 -0.0
639,678 + -1.9
639,689 + +1.1
639,789 - -1.8
639,996 - -1.6
640,000 + -0.1
640,001 - +0.1
640,173 + +1.1
640,173 + +0.4
640,174 - -0.2
640,174 - +0.1
640,200 + +0.1
640,200 + +0.2
640,200 + -1.7
640,200 + +0.4
640,201 - +0.4
640,201 - -2.0
640,201 - -1.1
640,201 - +1.7
640,201 - +0.7
640,437 + RR42_RS02870 0.18 +0.2
640,441 + RR42_RS02870 0.18 -0.9
640,441 + RR42_RS02870 0.18 -1.2
640,442 - RR42_RS02870 0.18 -1.7
640,792 - RR42_RS02870 0.39 -1.4
640,823 - RR42_RS02870 0.41 -0.9

Or see this region's nucleotide sequence