Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02545 and RR42_RS02550 are separated by 23 nucleotides RR42_RS02550 and RR42_RS02555 are separated by 88 nucleotides RR42_RS02555 and RR42_RS02560 are separated by 60 nucleotides RR42_RS02560 and RR42_RS02565 are separated by 174 nucleotides
RR42_RS02545: RR42_RS02545 - nitrate ABC transporter ATP-binding protein, at 573,303 to 574,166
_RS02545
RR42_RS02550: RR42_RS02550 - ABC transporter permease, at 574,190 to 574,954
_RS02550
RR42_RS02555: RR42_RS02555 - transcriptional regulator, at 575,043 to 575,501
_RS02555
RR42_RS02560: RR42_RS02560 - cysteine dioxygenase, at 575,562 to 576,158
_RS02560
RR42_RS02565: RR42_RS02565 - LysR family transcriptional regulator, at 576,333 to 577,304
_RS02565
Position (kb) 575
576 Strain fitness (log2 ratio) -2
-1
0
1 at 574.101 kb on - strand at 574.101 kb on - strand at 574.344 kb on - strand, within RR42_RS02550 at 574.375 kb on + strand, within RR42_RS02550 at 574.653 kb on + strand, within RR42_RS02550 at 574.654 kb on - strand, within RR42_RS02550 at 574.654 kb on - strand, within RR42_RS02550 at 574.654 kb on - strand, within RR42_RS02550 at 574.721 kb on + strand, within RR42_RS02550 at 574.721 kb on + strand, within RR42_RS02550 at 574.721 kb on + strand, within RR42_RS02550 at 574.898 kb on + strand at 574.898 kb on + strand at 574.899 kb on - strand at 574.899 kb on - strand at 574.899 kb on - strand at 574.899 kb on - strand at 574.899 kb on - strand at 575.050 kb on + strand at 575.050 kb on + strand at 575.074 kb on + strand at 575.143 kb on + strand, within RR42_RS02555 at 575.156 kb on - strand, within RR42_RS02555 at 575.216 kb on - strand, within RR42_RS02555 at 575.308 kb on - strand, within RR42_RS02555 at 575.362 kb on - strand, within RR42_RS02555 at 575.620 kb on + strand at 575.621 kb on - strand at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.914 kb on + strand, within RR42_RS02560 at 575.914 kb on + strand, within RR42_RS02560 at 575.915 kb on - strand, within RR42_RS02560 at 576.189 kb on + strand at 576.190 kb on - strand at 576.246 kb on + strand at 576.412 kb on + strand at 576.412 kb on + strand at 576.413 kb on - strand at 576.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 574,101 - -1.4 574,101 - -1.4 574,344 - RR42_RS02550 0.20 +1.5 574,375 + RR42_RS02550 0.24 +1.3 574,653 + RR42_RS02550 0.61 +1.3 574,654 - RR42_RS02550 0.61 -1.8 574,654 - RR42_RS02550 0.61 -1.4 574,654 - RR42_RS02550 0.61 -0.2 574,721 + RR42_RS02550 0.69 +0.1 574,721 + RR42_RS02550 0.69 -1.0 574,721 + RR42_RS02550 0.69 +0.7 574,898 + +1.1 574,898 + +0.3 574,899 - -1.4 574,899 - -1.5 574,899 - +0.2 574,899 - -1.6 574,899 - -0.3 575,050 + +1.2 575,050 + +0.2 575,074 + +0.7 575,143 + RR42_RS02555 0.22 -0.7 575,156 - RR42_RS02555 0.25 +0.2 575,216 - RR42_RS02555 0.38 +0.1 575,308 - RR42_RS02555 0.58 +1.4 575,362 - RR42_RS02555 0.69 -1.0 575,620 + +0.7 575,621 - +0.6 575,624 + RR42_RS02560 0.10 +0.9 575,624 + RR42_RS02560 0.10 -0.9 575,624 + RR42_RS02560 0.10 -0.0 575,624 + RR42_RS02560 0.10 -1.8 575,625 - RR42_RS02560 0.11 -0.5 575,625 - RR42_RS02560 0.11 -0.0 575,625 - RR42_RS02560 0.11 -2.2 575,898 + RR42_RS02560 0.56 -1.2 575,898 + RR42_RS02560 0.56 +0.9 575,898 + RR42_RS02560 0.56 +0.4 575,899 - RR42_RS02560 0.56 -1.7 575,899 - RR42_RS02560 0.56 -1.2 575,899 - RR42_RS02560 0.56 -1.5 575,914 + RR42_RS02560 0.59 -2.2 575,914 + RR42_RS02560 0.59 +0.9 575,915 - RR42_RS02560 0.59 -0.2 576,189 + -2.2 576,190 - -0.8 576,246 + -0.9 576,412 + +1.2 576,412 + +0.6 576,413 - -0.7 576,413 - -2.2
Or see this region's nucleotide sequence