Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02425 and RR42_RS02430 are separated by 448 nucleotides RR42_RS02430 and RR42_RS02435 are separated by 199 nucleotides RR42_RS02435 and RR42_RS02440 are separated by 14 nucleotides
RR42_RS02425: RR42_RS02425 - hypothetical protein, at 545,742 to 546,350
_RS02425
RR42_RS02430: RR42_RS02430 - sodium:solute symporter, at 546,799 to 548,235
_RS02430
RR42_RS02435: RR42_RS02435 - FmdB family transcriptional regulator, at 548,435 to 548,794
_RS02435
RR42_RS02440: RR42_RS02440 - membrane protein, at 548,809 to 549,507
_RS02440
Position (kb) 546
547
548
549 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 545.967 kb on - strand, within RR42_RS02425 at 546.038 kb on + strand, within RR42_RS02425 at 546.038 kb on + strand, within RR42_RS02425 at 546.039 kb on - strand, within RR42_RS02425 at 546.433 kb on + strand at 546.434 kb on - strand at 546.471 kb on + strand at 546.471 kb on + strand at 546.471 kb on + strand at 546.472 kb on - strand at 546.472 kb on - strand at 546.481 kb on - strand at 546.643 kb on - strand at 546.645 kb on - strand at 546.678 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.918 kb on + strand at 546.918 kb on + strand at 547.079 kb on - strand, within RR42_RS02430 at 547.108 kb on + strand, within RR42_RS02430 at 547.111 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.366 kb on + strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.367 kb on - strand, within RR42_RS02430 at 547.606 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.681 kb on + strand, within RR42_RS02430 at 547.682 kb on - strand, within RR42_RS02430 at 547.682 kb on - strand, within RR42_RS02430 at 547.682 kb on - strand, within RR42_RS02430 at 547.837 kb on - strand, within RR42_RS02430 at 547.849 kb on + strand, within RR42_RS02430 at 547.978 kb on + strand, within RR42_RS02430 at 547.979 kb on - strand, within RR42_RS02430 at 547.980 kb on + strand, within RR42_RS02430 at 547.981 kb on + strand, within RR42_RS02430 at 547.981 kb on - strand, within RR42_RS02430 at 547.981 kb on - strand, within RR42_RS02430 at 547.981 kb on - strand, within RR42_RS02430 at 548.434 kb on + strand at 548.444 kb on + strand at 549.093 kb on + strand, within RR42_RS02440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 545,967 - RR42_RS02425 0.37 +1.6 546,038 + RR42_RS02425 0.49 +0.2 546,038 + RR42_RS02425 0.49 -2.2 546,039 - RR42_RS02425 0.49 +1.1 546,433 + +1.2 546,434 - +1.0 546,471 + -0.6 546,471 + -1.6 546,471 + -0.2 546,472 - +0.9 546,472 - -0.8 546,481 - -0.8 546,643 - -1.3 546,645 - +1.0 546,678 + -0.2 546,916 + +0.2 546,916 + -0.2 546,916 + -2.6 546,916 + +3.6 546,916 + +0.6 546,916 + -2.0 546,917 - -3.3 546,917 - -0.6 546,917 - -0.1 546,917 - -2.2 546,917 - -0.6 546,918 + -1.4 546,918 + +0.0 547,079 - RR42_RS02430 0.19 -0.9 547,108 + RR42_RS02430 0.22 -1.5 547,111 + RR42_RS02430 0.22 -2.4 547,366 + RR42_RS02430 0.39 -2.6 547,366 + RR42_RS02430 0.39 +0.0 547,366 + RR42_RS02430 0.39 -2.4 547,366 + RR42_RS02430 0.39 +0.6 547,367 - RR42_RS02430 0.40 -0.6 547,367 - RR42_RS02430 0.40 -1.0 547,367 - RR42_RS02430 0.40 -0.5 547,367 - RR42_RS02430 0.40 -1.9 547,606 + RR42_RS02430 0.56 -0.2 547,681 + RR42_RS02430 0.61 -0.7 547,681 + RR42_RS02430 0.61 -0.4 547,681 + RR42_RS02430 0.61 -1.5 547,681 + RR42_RS02430 0.61 -1.9 547,682 - RR42_RS02430 0.61 +1.0 547,682 - RR42_RS02430 0.61 -0.3 547,682 - RR42_RS02430 0.61 -0.8 547,837 - RR42_RS02430 0.72 -0.1 547,849 + RR42_RS02430 0.73 -3.0 547,978 + RR42_RS02430 0.82 -3.0 547,979 - RR42_RS02430 0.82 -1.5 547,980 + RR42_RS02430 0.82 -1.1 547,981 + RR42_RS02430 0.82 -0.7 547,981 - RR42_RS02430 0.82 -1.6 547,981 - RR42_RS02430 0.82 -2.6 547,981 - RR42_RS02430 0.82 +0.1 548,434 + -0.8 548,444 + +0.2 549,093 + RR42_RS02440 0.41 -1.6
Or see this region's nucleotide sequence