Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02315 and RR42_RS02320 are separated by 243 nucleotides RR42_RS02320 and RR42_RS02325 are separated by 12 nucleotides
RR42_RS02315: RR42_RS02315 - threonine dehydratase, at 518,349 to 519,875
_RS02315
RR42_RS02320: RR42_RS02320 - trehalose phosphatase, at 520,119 to 520,904
_RS02320
RR42_RS02325: RR42_RS02325 - glucoamylase, at 520,917 to 522,755
_RS02325
Position (kb) 520
521 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 519.264 kb on + strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.296 kb on - strand, within RR42_RS02315 at 519.478 kb on + strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.491 kb on - strand, within RR42_RS02315 at 519.703 kb on + strand, within RR42_RS02315 at 519.797 kb on - strand at 519.797 kb on - strand at 519.826 kb on + strand at 519.826 kb on + strand at 519.856 kb on + strand at 519.856 kb on + strand at 519.857 kb on - strand at 519.918 kb on - strand at 519.918 kb on - strand at 520.066 kb on + strand at 520.112 kb on + strand at 520.113 kb on - strand at 520.130 kb on - strand at 520.497 kb on + strand, within RR42_RS02320 at 520.646 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.698 kb on - strand, within RR42_RS02320 at 521.169 kb on + strand, within RR42_RS02325 at 521.169 kb on + strand, within RR42_RS02325 at 521.170 kb on - strand, within RR42_RS02325 at 521.235 kb on - strand, within RR42_RS02325 at 521.271 kb on + strand, within RR42_RS02325 at 521.272 kb on - strand, within RR42_RS02325 at 521.406 kb on + strand, within RR42_RS02325 at 521.407 kb on - strand, within RR42_RS02325 at 521.408 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.458 kb on - strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.581 kb on - strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.829 kb on + strand, within RR42_RS02325 at 521.889 kb on + strand, within RR42_RS02325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 519,264 + RR42_RS02315 0.60 +1.0 519,265 - RR42_RS02315 0.60 -1.5 519,265 - RR42_RS02315 0.60 +0.3 519,296 - RR42_RS02315 0.62 +2.6 519,478 + RR42_RS02315 0.74 +0.1 519,479 - RR42_RS02315 0.74 +0.6 519,479 - RR42_RS02315 0.74 +0.7 519,479 - RR42_RS02315 0.74 +0.8 519,479 - RR42_RS02315 0.74 -0.5 519,491 - RR42_RS02315 0.75 +0.3 519,703 + RR42_RS02315 0.89 -0.9 519,797 - -0.3 519,797 - -0.2 519,826 + +1.1 519,826 + -0.3 519,856 + +0.0 519,856 + -2.0 519,857 - -0.8 519,918 - -0.9 519,918 - -0.8 520,066 + -0.6 520,112 + +0.3 520,113 - +1.0 520,130 - +2.3 520,497 + RR42_RS02320 0.48 -0.7 520,646 + RR42_RS02320 0.67 -1.3 520,697 + RR42_RS02320 0.74 -0.4 520,697 + RR42_RS02320 0.74 +0.6 520,698 - RR42_RS02320 0.74 -0.9 521,169 + RR42_RS02325 0.14 -0.3 521,169 + RR42_RS02325 0.14 -0.5 521,170 - RR42_RS02325 0.14 +0.1 521,235 - RR42_RS02325 0.17 +0.1 521,271 + RR42_RS02325 0.19 -0.3 521,272 - RR42_RS02325 0.19 -0.9 521,406 + RR42_RS02325 0.27 +0.8 521,407 - RR42_RS02325 0.27 +0.6 521,408 + RR42_RS02325 0.27 -0.4 521,457 + RR42_RS02325 0.29 -0.1 521,457 + RR42_RS02325 0.29 -0.2 521,457 + RR42_RS02325 0.29 +0.3 521,457 + RR42_RS02325 0.29 -1.4 521,458 - RR42_RS02325 0.29 +0.9 521,580 + RR42_RS02325 0.36 +3.3 521,580 + RR42_RS02325 0.36 -0.2 521,580 + RR42_RS02325 0.36 -2.1 521,581 - RR42_RS02325 0.36 -0.5 521,651 + RR42_RS02325 0.40 +0.2 521,651 + RR42_RS02325 0.40 -0.3 521,671 - RR42_RS02325 0.41 -1.0 521,671 - RR42_RS02325 0.41 +1.8 521,829 + RR42_RS02325 0.50 +1.8 521,889 + RR42_RS02325 0.53 +0.1
Or see this region's nucleotide sequence