Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02175 and RR42_RS02180 are separated by 8 nucleotides RR42_RS02180 and RR42_RS02185 are separated by 26 nucleotides
RR42_RS02175: RR42_RS02175 - acetolactate synthase, at 483,230 to 484,855
_RS02175
RR42_RS02180: RR42_RS02180 - ABC transporter substrate-binding protein, at 484,864 to 485,850
_RS02180
RR42_RS02185: RR42_RS02185 - fumarate reductase, at 485,877 to 487,604
_RS02185
Position (kb)
484
485
486 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 484.073 kb on + strand, within RR42_RS02175 at 484.073 kb on + strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.597 kb on - strand, within RR42_RS02175 at 484.788 kb on - strand at 484.788 kb on - strand at 484.865 kb on + strand at 484.866 kb on - strand at 484.904 kb on + strand at 484.904 kb on + strand at 484.905 kb on - strand at 485.072 kb on + strand, within RR42_RS02180 at 485.101 kb on + strand, within RR42_RS02180 at 485.202 kb on + strand, within RR42_RS02180 at 485.257 kb on + strand, within RR42_RS02180 at 485.268 kb on + strand, within RR42_RS02180 at 485.269 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.519 kb on + strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.751 kb on + strand, within RR42_RS02180 at 485.866 kb on + strand at 485.867 kb on - strand at 485.874 kb on - strand at 486.547 kb on + strand, within RR42_RS02185 at 486.548 kb on - strand, within RR42_RS02185 at 486.548 kb on - strand, within RR42_RS02185 at 486.673 kb on + strand, within RR42_RS02185 at 486.674 kb on - strand, within RR42_RS02185 at 486.674 kb on - strand, within RR42_RS02185 at 486.718 kb on + strand, within RR42_RS02185 at 486.719 kb on - strand, within RR42_RS02185 at 486.719 kb on - strand, within RR42_RS02185 at 486.770 kb on - strand, within RR42_RS02185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 484,073 + RR42_RS02175 0.52 -1.1 484,073 + RR42_RS02175 0.52 -0.9 484,074 - RR42_RS02175 0.52 +2.6 484,074 - RR42_RS02175 0.52 -1.8 484,597 - RR42_RS02175 0.84 +0.1 484,788 - -1.7 484,788 - +0.1 484,865 + +1.0 484,866 - -0.6 484,904 + +0.2 484,904 + -0.7 484,905 - +1.9 485,072 + RR42_RS02180 0.21 +1.0 485,101 + RR42_RS02180 0.24 -0.4 485,202 + RR42_RS02180 0.34 -1.7 485,257 + RR42_RS02180 0.40 -0.3 485,268 + RR42_RS02180 0.41 +1.0 485,269 - RR42_RS02180 0.41 -0.8 485,300 - RR42_RS02180 0.44 -1.3 485,300 - RR42_RS02180 0.44 -0.4 485,384 + RR42_RS02180 0.53 +1.5 485,384 + RR42_RS02180 0.53 +0.1 485,384 + RR42_RS02180 0.53 -0.1 485,384 + RR42_RS02180 0.53 +0.2 485,385 - RR42_RS02180 0.53 +0.0 485,385 - RR42_RS02180 0.53 -0.3 485,519 + RR42_RS02180 0.66 -0.4 485,520 - RR42_RS02180 0.66 +0.2 485,520 - RR42_RS02180 0.66 -0.1 485,520 - RR42_RS02180 0.66 +0.6 485,736 - RR42_RS02180 0.88 +0.0 485,736 - RR42_RS02180 0.88 -1.1 485,736 - RR42_RS02180 0.88 -0.6 485,751 + RR42_RS02180 0.90 -0.1 485,866 + -1.9 485,867 - +0.4 485,874 - +0.9 486,547 + RR42_RS02185 0.39 -1.4 486,548 - RR42_RS02185 0.39 -0.2 486,548 - RR42_RS02185 0.39 +1.2 486,673 + RR42_RS02185 0.46 -0.4 486,674 - RR42_RS02185 0.46 -0.6 486,674 - RR42_RS02185 0.46 -0.4 486,718 + RR42_RS02185 0.49 +0.0 486,719 - RR42_RS02185 0.49 -2.0 486,719 - RR42_RS02185 0.49 +1.1 486,770 - RR42_RS02185 0.52 -0.4
Or see this region's nucleotide sequence