Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02160 and RR42_RS02165 are separated by 102 nucleotides RR42_RS02165 and RR42_RS02170 are separated by 215 nucleotides RR42_RS02170 and RR42_RS02175 are separated by 20 nucleotides
RR42_RS02160: RR42_RS02160 - LacI family transcriptional regulator, at 480,232 to 481,215
_RS02160
RR42_RS02165: RR42_RS02165 - LysR family transcriptional regulator, at 481,318 to 482,256
_RS02165
RR42_RS02170: RR42_RS02170 - hypothetical protein, at 482,472 to 483,209
_RS02170
RR42_RS02175: RR42_RS02175 - acetolactate synthase, at 483,230 to 484,855
_RS02175
Position (kb) 481
482
483 Strain fitness (log2 ratio) -2
-1
0
1
2 at 480.321 kb on + strand at 480.450 kb on - strand, within RR42_RS02160 at 480.450 kb on - strand, within RR42_RS02160 at 480.460 kb on + strand, within RR42_RS02160 at 480.460 kb on + strand, within RR42_RS02160 at 480.461 kb on - strand, within RR42_RS02160 at 480.461 kb on - strand, within RR42_RS02160 at 480.653 kb on + strand, within RR42_RS02160 at 480.653 kb on + strand, within RR42_RS02160 at 480.653 kb on + strand, within RR42_RS02160 at 480.654 kb on - strand, within RR42_RS02160 at 480.654 kb on - strand, within RR42_RS02160 at 480.654 kb on - strand, within RR42_RS02160 at 480.741 kb on - strand, within RR42_RS02160 at 480.770 kb on + strand, within RR42_RS02160 at 480.771 kb on - strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.894 kb on + strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 481.149 kb on + strand at 481.149 kb on + strand at 481.150 kb on - strand at 481.271 kb on + strand at 481.271 kb on + strand at 481.272 kb on - strand at 481.275 kb on + strand at 481.275 kb on + strand at 481.276 kb on - strand at 481.810 kb on - strand, within RR42_RS02165 at 481.810 kb on - strand, within RR42_RS02165 at 481.810 kb on - strand, within RR42_RS02165 at 481.829 kb on - strand, within RR42_RS02165 at 481.914 kb on - strand, within RR42_RS02165 at 482.261 kb on - strand at 482.293 kb on + strand at 482.360 kb on - strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.399 kb on - strand at 482.435 kb on + strand at 482.521 kb on - strand at 482.521 kb on - strand at 482.522 kb on + strand at 482.869 kb on + strand, within RR42_RS02170 at 482.869 kb on + strand, within RR42_RS02170 at 482.872 kb on + strand, within RR42_RS02170 at 482.916 kb on + strand, within RR42_RS02170 at 482.917 kb on - strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 483.033 kb on + strand, within RR42_RS02170 at 483.034 kb on - strand, within RR42_RS02170 at 483.034 kb on - strand, within RR42_RS02170 at 483.041 kb on + strand, within RR42_RS02170 at 483.093 kb on + strand, within RR42_RS02170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 480,321 + -0.1 480,450 - RR42_RS02160 0.22 -0.2 480,450 - RR42_RS02160 0.22 -1.0 480,460 + RR42_RS02160 0.23 -1.6 480,460 + RR42_RS02160 0.23 +1.1 480,461 - RR42_RS02160 0.23 -1.0 480,461 - RR42_RS02160 0.23 -0.2 480,653 + RR42_RS02160 0.43 -0.4 480,653 + RR42_RS02160 0.43 -1.6 480,653 + RR42_RS02160 0.43 -0.6 480,654 - RR42_RS02160 0.43 -0.9 480,654 - RR42_RS02160 0.43 -1.5 480,654 - RR42_RS02160 0.43 +0.9 480,741 - RR42_RS02160 0.52 -0.5 480,770 + RR42_RS02160 0.55 -0.5 480,771 - RR42_RS02160 0.55 -1.3 480,893 + RR42_RS02160 0.67 +1.0 480,893 + RR42_RS02160 0.67 +0.1 480,893 + RR42_RS02160 0.67 +0.0 480,893 + RR42_RS02160 0.67 -0.7 480,894 + RR42_RS02160 0.67 -0.7 480,942 - RR42_RS02160 0.72 +0.1 480,942 - RR42_RS02160 0.72 -0.9 480,942 - RR42_RS02160 0.72 +1.2 480,942 - RR42_RS02160 0.72 -1.0 481,149 + +2.2 481,149 + +0.5 481,150 - -1.1 481,271 + +0.6 481,271 + +0.8 481,272 - +0.4 481,275 + +0.0 481,275 + -0.2 481,276 - +0.7 481,810 - RR42_RS02165 0.52 +1.1 481,810 - RR42_RS02165 0.52 -0.9 481,810 - RR42_RS02165 0.52 -0.4 481,829 - RR42_RS02165 0.54 -2.1 481,914 - RR42_RS02165 0.63 -1.3 482,261 - -0.6 482,293 + -0.7 482,360 - +0.3 482,397 + -1.7 482,397 + +0.2 482,397 + -0.9 482,397 + -0.4 482,398 - -0.7 482,398 - -0.4 482,398 - -1.6 482,398 - +0.5 482,398 - -0.3 482,399 - +2.1 482,435 + -0.3 482,521 - +0.9 482,521 - +0.4 482,522 + -2.4 482,869 + RR42_RS02170 0.54 +0.3 482,869 + RR42_RS02170 0.54 -0.6 482,872 + RR42_RS02170 0.54 -0.5 482,916 + RR42_RS02170 0.60 +1.4 482,917 - RR42_RS02170 0.60 +0.3 482,925 + RR42_RS02170 0.61 +0.2 482,925 + RR42_RS02170 0.61 -1.1 482,925 + RR42_RS02170 0.61 -0.8 483,033 + RR42_RS02170 0.76 -1.7 483,034 - RR42_RS02170 0.76 -1.5 483,034 - RR42_RS02170 0.76 -1.2 483,041 + RR42_RS02170 0.77 -0.1 483,093 + RR42_RS02170 0.84 -1.4
Or see this region's nucleotide sequence