Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02045 and RR42_RS02050 are separated by 99 nucleotides RR42_RS02050 and RR42_RS02055 are separated by 6 nucleotides RR42_RS02055 and RR42_RS02060 are separated by 66 nucleotides
RR42_RS02045: RR42_RS02045 - GTPase, at 455,455 to 457,398
_RS02045
RR42_RS02050: RR42_RS02050 - adenine glycosylase, at 457,498 to 458,667
_RS02050
RR42_RS02055: RR42_RS02055 - ATP-dependent protease, at 458,674 to 459,351
_RS02055
RR42_RS02060: RR42_RS02060 - nucleotide-binding protein, at 459,418 to 460,335
_RS02060
Position (kb)
457
458
459 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 456.928 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 457.059 kb on + strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.107 kb on + strand, within RR42_RS02045 at 457.295 kb on + strand at 457.295 kb on + strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.298 kb on + strand at 457.298 kb on + strand at 457.299 kb on - strand at 457.492 kb on + strand at 457.642 kb on + strand, within RR42_RS02050 at 457.702 kb on + strand, within RR42_RS02050 at 457.703 kb on - strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.798 kb on + strand, within RR42_RS02050 at 457.799 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.463 kb on + strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.606 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.703 kb on + strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.881 kb on - strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.055 kb on - strand, within RR42_RS02055 at 459.055 kb on - strand, within RR42_RS02055 at 459.197 kb on + strand, within RR42_RS02055 at 459.197 kb on + strand, within RR42_RS02055 at 459.198 kb on - strand, within RR42_RS02055 at 459.198 kb on - strand, within RR42_RS02055 at 459.244 kb on + strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.249 kb on + strand, within RR42_RS02055 at 459.283 kb on - strand, within RR42_RS02055 at 459.459 kb on + strand at 459.459 kb on + strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.477 kb on - strand at 459.500 kb on + strand at 459.500 kb on + strand at 459.500 kb on + strand at 459.501 kb on - strand at 459.501 kb on - strand at 459.549 kb on - strand, within RR42_RS02060 at 459.549 kb on - strand, within RR42_RS02060 at 459.594 kb on - strand, within RR42_RS02060 at 459.594 kb on - strand, within RR42_RS02060 at 459.601 kb on + strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 456,928 + RR42_RS02045 0.76 +0.3 456,928 + RR42_RS02045 0.76 +1.6 456,929 - RR42_RS02045 0.76 +0.8 456,929 - RR42_RS02045 0.76 +2.7 456,929 - RR42_RS02045 0.76 -0.6 456,957 + RR42_RS02045 0.77 +2.5 456,957 + RR42_RS02045 0.77 +1.0 456,957 + RR42_RS02045 0.77 +1.4 456,957 + RR42_RS02045 0.77 +2.2 456,958 - RR42_RS02045 0.77 +1.6 456,958 - RR42_RS02045 0.77 +1.1 456,958 - RR42_RS02045 0.77 +2.0 456,958 - RR42_RS02045 0.77 +2.7 456,958 - RR42_RS02045 0.77 -1.3 456,994 + RR42_RS02045 0.79 +2.2 456,994 + RR42_RS02045 0.79 -0.4 456,994 + RR42_RS02045 0.79 +2.0 456,994 + RR42_RS02045 0.79 +3.2 456,995 - RR42_RS02045 0.79 +2.0 456,995 - RR42_RS02045 0.79 +3.0 457,059 + RR42_RS02045 0.83 +2.1 457,060 - RR42_RS02045 0.83 +0.7 457,060 - RR42_RS02045 0.83 +2.4 457,107 + RR42_RS02045 0.85 +0.8 457,295 + +3.3 457,295 + +3.1 457,296 - +2.1 457,296 - +1.3 457,296 - +3.8 457,298 + +4.3 457,298 + +3.4 457,299 - +4.1 457,492 + +0.2 457,642 + RR42_RS02050 0.12 +0.4 457,702 + RR42_RS02050 0.17 -0.5 457,703 - RR42_RS02050 0.18 +0.8 457,795 + RR42_RS02050 0.25 -1.6 457,795 + RR42_RS02050 0.25 +0.1 457,798 + RR42_RS02050 0.26 +0.0 457,799 - RR42_RS02050 0.26 +0.3 458,108 - RR42_RS02050 0.52 -0.9 458,108 - RR42_RS02050 0.52 +0.3 458,108 - RR42_RS02050 0.52 -0.3 458,463 + RR42_RS02050 0.82 +1.7 458,464 - RR42_RS02050 0.83 +0.9 458,464 - RR42_RS02050 0.83 -1.2 458,464 - RR42_RS02050 0.83 -2.6 458,606 + -0.4 458,699 + -2.2 458,699 + -0.3 458,699 + -1.7 458,699 + -0.5 458,699 + -0.0 458,699 + +0.6 458,699 + -1.7 458,699 + -0.8 458,699 + -0.9 458,700 - -0.4 458,700 - -2.2 458,700 - -0.4 458,700 - -2.2 458,700 - -0.8 458,700 - +1.2 458,700 - +0.7 458,703 + -1.0 458,704 - +0.6 458,704 - +0.7 458,704 - +1.4 458,704 - -0.2 458,881 - RR42_RS02055 0.31 -1.0 459,054 + RR42_RS02055 0.56 -2.0 459,054 + RR42_RS02055 0.56 -0.1 459,054 + RR42_RS02055 0.56 +0.4 459,054 + RR42_RS02055 0.56 +1.1 459,055 - RR42_RS02055 0.56 +1.7 459,055 - RR42_RS02055 0.56 +0.1 459,197 + RR42_RS02055 0.77 -2.2 459,197 + RR42_RS02055 0.77 +0.6 459,198 - RR42_RS02055 0.77 -1.7 459,198 - RR42_RS02055 0.77 -1.0 459,244 + RR42_RS02055 0.84 +1.8 459,245 - RR42_RS02055 0.84 -0.2 459,245 - RR42_RS02055 0.84 -0.2 459,245 - RR42_RS02055 0.84 +1.3 459,249 + RR42_RS02055 0.85 +0.9 459,283 - RR42_RS02055 0.90 -1.0 459,459 + +0.7 459,459 + +0.4 459,460 - -0.4 459,460 - -1.5 459,460 - -2.1 459,460 - +0.6 459,460 - +1.0 459,476 + -1.3 459,476 + -0.4 459,476 + -0.4 459,476 + +0.1 459,477 - +1.6 459,500 + +0.6 459,500 + -1.3 459,500 + -1.3 459,501 - -0.9 459,501 - +1.6 459,549 - RR42_RS02060 0.14 -3.0 459,549 - RR42_RS02060 0.14 -2.6 459,594 - RR42_RS02060 0.19 -2.9 459,594 - RR42_RS02060 0.19 -0.0 459,601 + RR42_RS02060 0.20 -0.6 459,602 - RR42_RS02060 0.20 -1.7 459,602 - RR42_RS02060 0.20 -1.3 459,602 - RR42_RS02060 0.20 -2.3 459,602 - RR42_RS02060 0.20 -1.1 459,602 - RR42_RS02060 0.20 +3.2
Or see this region's nucleotide sequence