Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02040 and RR42_RS02045 are separated by 283 nucleotides RR42_RS02045 and RR42_RS02050 are separated by 99 nucleotides
RR42_RS02040: RR42_RS02040 - formamidopyrimidine-DNA glycosylase, at 454,290 to 455,171
_RS02040
RR42_RS02045: RR42_RS02045 - GTPase, at 455,455 to 457,398
_RS02045
RR42_RS02050: RR42_RS02050 - adenine glycosylase, at 457,498 to 458,667
_RS02050
Position (kb)
455
456
457
458 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 454.464 kb on + strand, within RR42_RS02040 at 454.464 kb on + strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 455.071 kb on - strand, within RR42_RS02040 at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.214 kb on - strand at 455.232 kb on + strand at 455.232 kb on + strand at 455.233 kb on - strand at 455.327 kb on - strand at 455.327 kb on - strand at 455.351 kb on + strand at 455.352 kb on - strand at 455.485 kb on + strand at 455.485 kb on + strand at 455.486 kb on - strand at 455.508 kb on - strand at 455.508 kb on - strand at 455.557 kb on + strand at 455.590 kb on - strand at 455.727 kb on + strand at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.753 kb on - strand, within RR42_RS02045 at 455.853 kb on + strand, within RR42_RS02045 at 455.854 kb on - strand, within RR42_RS02045 at 455.854 kb on - strand, within RR42_RS02045 at 455.959 kb on + strand, within RR42_RS02045 at 455.959 kb on + strand, within RR42_RS02045 at 455.960 kb on - strand, within RR42_RS02045 at 455.960 kb on - strand, within RR42_RS02045 at 456.376 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 457.059 kb on + strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.107 kb on + strand, within RR42_RS02045 at 457.295 kb on + strand at 457.295 kb on + strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.298 kb on + strand at 457.298 kb on + strand at 457.299 kb on - strand at 457.492 kb on + strand at 457.642 kb on + strand, within RR42_RS02050 at 457.702 kb on + strand, within RR42_RS02050 at 457.703 kb on - strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.798 kb on + strand, within RR42_RS02050 at 457.799 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 454,464 + RR42_RS02040 0.20 +0.3 454,464 + RR42_RS02040 0.20 +1.0 454,465 - RR42_RS02040 0.20 -0.7 454,465 - RR42_RS02040 0.20 -0.4 454,792 - RR42_RS02040 0.57 +1.4 454,792 - RR42_RS02040 0.57 +0.6 454,868 + RR42_RS02040 0.66 +0.9 454,868 + RR42_RS02040 0.66 +0.0 454,868 + RR42_RS02040 0.66 +0.6 454,868 + RR42_RS02040 0.66 +1.9 454,868 + RR42_RS02040 0.66 +1.1 454,869 - RR42_RS02040 0.66 +0.6 454,869 - RR42_RS02040 0.66 +0.5 454,869 - RR42_RS02040 0.66 +0.2 454,869 - RR42_RS02040 0.66 +0.1 454,869 - RR42_RS02040 0.66 -1.6 454,869 - RR42_RS02040 0.66 -2.0 454,869 - RR42_RS02040 0.66 +1.1 454,869 - RR42_RS02040 0.66 +0.9 454,872 + RR42_RS02040 0.66 -1.2 454,872 + RR42_RS02040 0.66 -0.2 454,872 + RR42_RS02040 0.66 -0.2 454,872 + RR42_RS02040 0.66 -2.8 454,872 + RR42_RS02040 0.66 -1.6 454,872 + RR42_RS02040 0.66 +0.8 454,872 + RR42_RS02040 0.66 -1.6 454,872 + RR42_RS02040 0.66 +2.0 454,872 + RR42_RS02040 0.66 -0.3 454,872 + RR42_RS02040 0.66 -0.5 454,872 + RR42_RS02040 0.66 -0.0 454,872 + RR42_RS02040 0.66 -0.2 454,873 - RR42_RS02040 0.66 -1.4 454,873 - RR42_RS02040 0.66 -1.2 454,873 - RR42_RS02040 0.66 -0.2 454,873 - RR42_RS02040 0.66 +0.2 454,873 - RR42_RS02040 0.66 -0.3 454,873 - RR42_RS02040 0.66 -0.4 454,873 - RR42_RS02040 0.66 -0.2 454,873 - RR42_RS02040 0.66 -0.4 454,873 - RR42_RS02040 0.66 +1.0 455,071 - RR42_RS02040 0.89 -2.3 455,148 + -0.6 455,148 + -0.5 455,148 + -1.4 455,148 + +0.5 455,214 - -0.0 455,232 + +0.5 455,232 + -1.6 455,233 - -2.9 455,327 - -0.1 455,327 - +1.9 455,351 + +0.9 455,352 - +4.2 455,485 + +3.6 455,485 + +1.6 455,486 - +1.4 455,508 - -1.0 455,508 - +2.9 455,557 + +2.6 455,590 - +1.6 455,727 + +1.3 455,727 + RR42_RS02045 0.14 +1.5 455,727 + RR42_RS02045 0.14 +1.6 455,727 + RR42_RS02045 0.14 +0.7 455,727 + RR42_RS02045 0.14 +2.4 455,728 - RR42_RS02045 0.14 -1.1 455,728 - RR42_RS02045 0.14 +1.7 455,728 - RR42_RS02045 0.14 +2.6 455,728 - RR42_RS02045 0.14 +1.5 455,728 - RR42_RS02045 0.14 +1.7 455,753 - RR42_RS02045 0.15 +2.4 455,853 + RR42_RS02045 0.20 -1.7 455,854 - RR42_RS02045 0.21 +2.2 455,854 - RR42_RS02045 0.21 +1.6 455,959 + RR42_RS02045 0.26 +0.6 455,959 + RR42_RS02045 0.26 +0.9 455,960 - RR42_RS02045 0.26 +2.6 455,960 - RR42_RS02045 0.26 +1.8 456,376 + RR42_RS02045 0.47 +2.1 456,928 + RR42_RS02045 0.76 +0.3 456,928 + RR42_RS02045 0.76 +1.6 456,929 - RR42_RS02045 0.76 +0.8 456,929 - RR42_RS02045 0.76 +2.7 456,929 - RR42_RS02045 0.76 -0.6 456,957 + RR42_RS02045 0.77 +2.5 456,957 + RR42_RS02045 0.77 +1.0 456,957 + RR42_RS02045 0.77 +1.4 456,957 + RR42_RS02045 0.77 +2.2 456,958 - RR42_RS02045 0.77 +1.6 456,958 - RR42_RS02045 0.77 +1.1 456,958 - RR42_RS02045 0.77 +2.0 456,958 - RR42_RS02045 0.77 +2.7 456,958 - RR42_RS02045 0.77 -1.3 456,994 + RR42_RS02045 0.79 +2.2 456,994 + RR42_RS02045 0.79 -0.4 456,994 + RR42_RS02045 0.79 +2.0 456,994 + RR42_RS02045 0.79 +3.2 456,995 - RR42_RS02045 0.79 +2.0 456,995 - RR42_RS02045 0.79 +3.0 457,059 + RR42_RS02045 0.83 +2.1 457,060 - RR42_RS02045 0.83 +0.7 457,060 - RR42_RS02045 0.83 +2.4 457,107 + RR42_RS02045 0.85 +0.8 457,295 + +3.3 457,295 + +3.1 457,296 - +2.1 457,296 - +1.3 457,296 - +3.8 457,298 + +4.3 457,298 + +3.4 457,299 - +4.1 457,492 + +0.2 457,642 + RR42_RS02050 0.12 +0.4 457,702 + RR42_RS02050 0.17 -0.5 457,703 - RR42_RS02050 0.18 +0.8 457,795 + RR42_RS02050 0.25 -1.6 457,795 + RR42_RS02050 0.25 +0.1 457,798 + RR42_RS02050 0.26 +0.0 457,799 - RR42_RS02050 0.26 +0.3 458,108 - RR42_RS02050 0.52 -0.9 458,108 - RR42_RS02050 0.52 +0.3 458,108 - RR42_RS02050 0.52 -0.3
Or see this region's nucleotide sequence