Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01230 and RR42_RS01235 are separated by 86 nucleotides RR42_RS01235 and RR42_RS01240 are separated by 122 nucleotides
RR42_RS01230: RR42_RS01230 - acetoacetate decarboxylase, at 278,651 to 279,391
_RS01230
RR42_RS01235: RR42_RS01235 - 3-hydroxybutyrate dehydrogenase, at 279,478 to 280,260
_RS01235
RR42_RS01240: RR42_RS01240 - membrane protein, at 280,383 to 281,735
_RS01240
Position (kb)
279
280
281 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 278.696 kb on - strand at 278.831 kb on + strand, within RR42_RS01230 at 278.832 kb on - strand, within RR42_RS01230 at 278.873 kb on + strand, within RR42_RS01230 at 278.874 kb on - strand, within RR42_RS01230 at 278.874 kb on - strand, within RR42_RS01230 at 278.906 kb on + strand, within RR42_RS01230 at 278.906 kb on + strand, within RR42_RS01230 at 278.927 kb on + strand, within RR42_RS01230 at 278.927 kb on + strand, within RR42_RS01230 at 278.927 kb on + strand, within RR42_RS01230 at 278.928 kb on - strand, within RR42_RS01230 at 278.928 kb on - strand, within RR42_RS01230 at 278.942 kb on + strand, within RR42_RS01230 at 278.943 kb on - strand, within RR42_RS01230 at 278.983 kb on - strand, within RR42_RS01230 at 278.983 kb on - strand, within RR42_RS01230 at 279.034 kb on + strand, within RR42_RS01230 at 279.034 kb on + strand, within RR42_RS01230 at 279.056 kb on + strand, within RR42_RS01230 at 279.092 kb on + strand, within RR42_RS01230 at 279.092 kb on + strand, within RR42_RS01230 at 279.152 kb on + strand, within RR42_RS01230 at 279.153 kb on - strand, within RR42_RS01230 at 279.212 kb on + strand, within RR42_RS01230 at 279.212 kb on + strand, within RR42_RS01230 at 279.212 kb on + strand, within RR42_RS01230 at 279.213 kb on - strand, within RR42_RS01230 at 279.213 kb on - strand, within RR42_RS01230 at 279.335 kb on + strand at 279.336 kb on - strand at 279.355 kb on - strand at 279.380 kb on + strand at 279.778 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.862 kb on + strand, within RR42_RS01235 at 279.862 kb on + strand, within RR42_RS01235 at 279.863 kb on - strand, within RR42_RS01235 at 279.863 kb on - strand, within RR42_RS01235 at 279.863 kb on - strand, within RR42_RS01235 at 279.892 kb on + strand, within RR42_RS01235 at 279.892 kb on + strand, within RR42_RS01235 at 279.893 kb on - strand, within RR42_RS01235 at 279.893 kb on - strand, within RR42_RS01235 at 279.893 kb on - strand, within RR42_RS01235 at 279.940 kb on + strand, within RR42_RS01235 at 279.940 kb on + strand, within RR42_RS01235 at 279.941 kb on - strand, within RR42_RS01235 at 280.004 kb on - strand, within RR42_RS01235 at 280.004 kb on - strand, within RR42_RS01235 at 280.231 kb on - strand at 280.249 kb on + strand at 280.249 kb on + strand at 280.249 kb on + strand at 280.250 kb on - strand at 280.250 kb on - strand at 280.259 kb on - strand at 280.417 kb on + strand at 280.418 kb on - strand at 280.418 kb on - strand at 280.418 kb on - strand at 280.441 kb on + strand at 280.442 kb on - strand at 280.593 kb on + strand, within RR42_RS01240 at 280.593 kb on + strand, within RR42_RS01240 at 280.593 kb on + strand, within RR42_RS01240 at 280.641 kb on + strand, within RR42_RS01240 at 280.656 kb on + strand, within RR42_RS01240 at 280.688 kb on + strand, within RR42_RS01240 at 280.689 kb on - strand, within RR42_RS01240 at 280.788 kb on + strand, within RR42_RS01240 at 280.789 kb on - strand, within RR42_RS01240 at 280.789 kb on - strand, within RR42_RS01240 at 280.827 kb on + strand, within RR42_RS01240 at 280.992 kb on + strand, within RR42_RS01240 at 280.992 kb on + strand, within RR42_RS01240 at 280.995 kb on + strand, within RR42_RS01240 at 280.995 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.119 kb on - strand, within RR42_RS01240 at 281.218 kb on - strand, within RR42_RS01240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 278,696 - +0.5 278,831 + RR42_RS01230 0.24 +1.1 278,832 - RR42_RS01230 0.24 -1.5 278,873 + RR42_RS01230 0.30 +0.1 278,874 - RR42_RS01230 0.30 +1.3 278,874 - RR42_RS01230 0.30 -0.2 278,906 + RR42_RS01230 0.34 +0.2 278,906 + RR42_RS01230 0.34 +0.6 278,927 + RR42_RS01230 0.37 +0.4 278,927 + RR42_RS01230 0.37 -0.8 278,927 + RR42_RS01230 0.37 -0.5 278,928 - RR42_RS01230 0.37 +1.6 278,928 - RR42_RS01230 0.37 +0.6 278,942 + RR42_RS01230 0.39 -0.5 278,943 - RR42_RS01230 0.39 -0.8 278,983 - RR42_RS01230 0.45 +0.3 278,983 - RR42_RS01230 0.45 +0.1 279,034 + RR42_RS01230 0.52 -0.1 279,034 + RR42_RS01230 0.52 -1.6 279,056 + RR42_RS01230 0.55 +0.4 279,092 + RR42_RS01230 0.60 -1.5 279,092 + RR42_RS01230 0.60 -0.3 279,152 + RR42_RS01230 0.68 +0.1 279,153 - RR42_RS01230 0.68 -0.5 279,212 + RR42_RS01230 0.76 -0.7 279,212 + RR42_RS01230 0.76 -0.7 279,212 + RR42_RS01230 0.76 -0.5 279,213 - RR42_RS01230 0.76 +0.2 279,213 - RR42_RS01230 0.76 -2.0 279,335 + +0.0 279,336 - +1.6 279,355 - -1.0 279,380 + +0.4 279,778 + RR42_RS01235 0.38 +1.1 279,858 + RR42_RS01235 0.49 -2.5 279,858 + RR42_RS01235 0.49 +0.3 279,858 + RR42_RS01235 0.49 +0.6 279,858 + RR42_RS01235 0.49 +1.1 279,858 + RR42_RS01235 0.49 +0.5 279,859 - RR42_RS01235 0.49 -1.2 279,859 - RR42_RS01235 0.49 -2.6 279,859 - RR42_RS01235 0.49 -0.4 279,859 - RR42_RS01235 0.49 +0.8 279,859 - RR42_RS01235 0.49 +1.2 279,859 - RR42_RS01235 0.49 +0.2 279,859 - RR42_RS01235 0.49 +0.5 279,859 - RR42_RS01235 0.49 +0.1 279,859 - RR42_RS01235 0.49 +0.4 279,862 + RR42_RS01235 0.49 -0.8 279,862 + RR42_RS01235 0.49 -0.4 279,863 - RR42_RS01235 0.49 -0.8 279,863 - RR42_RS01235 0.49 -1.3 279,863 - RR42_RS01235 0.49 +0.4 279,892 + RR42_RS01235 0.53 +0.0 279,892 + RR42_RS01235 0.53 -1.0 279,893 - RR42_RS01235 0.53 +0.3 279,893 - RR42_RS01235 0.53 +2.4 279,893 - RR42_RS01235 0.53 +0.7 279,940 + RR42_RS01235 0.59 +1.0 279,940 + RR42_RS01235 0.59 -0.4 279,941 - RR42_RS01235 0.59 -0.7 280,004 - RR42_RS01235 0.67 -0.4 280,004 - RR42_RS01235 0.67 -0.3 280,231 - -0.6 280,249 + -0.9 280,249 + -0.4 280,249 + -1.4 280,250 - -0.6 280,250 - +0.2 280,259 - -0.7 280,417 + -0.2 280,418 - -0.9 280,418 - -0.7 280,418 - +0.5 280,441 + -0.7 280,442 - -0.3 280,593 + RR42_RS01240 0.16 -1.9 280,593 + RR42_RS01240 0.16 -0.9 280,593 + RR42_RS01240 0.16 -1.0 280,641 + RR42_RS01240 0.19 +0.1 280,656 + RR42_RS01240 0.20 -2.0 280,688 + RR42_RS01240 0.23 +0.4 280,689 - RR42_RS01240 0.23 +0.4 280,788 + RR42_RS01240 0.30 +0.4 280,789 - RR42_RS01240 0.30 +1.4 280,789 - RR42_RS01240 0.30 +1.4 280,827 + RR42_RS01240 0.33 -2.3 280,992 + RR42_RS01240 0.45 -1.6 280,992 + RR42_RS01240 0.45 +2.4 280,995 + RR42_RS01240 0.45 -2.6 280,995 + RR42_RS01240 0.45 +0.4 281,000 + RR42_RS01240 0.46 -1.1 281,000 + RR42_RS01240 0.46 +0.7 281,000 + RR42_RS01240 0.46 -1.4 281,000 + RR42_RS01240 0.46 -2.7 281,000 + RR42_RS01240 0.46 +1.6 281,119 - RR42_RS01240 0.54 -0.6 281,218 - RR42_RS01240 0.62 +0.5
Or see this region's nucleotide sequence