Experiment: R2A with Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00195 and RR42_RS00200 are separated by 58 nucleotides RR42_RS00200 and RR42_RS00205 overlap by 4 nucleotides RR42_RS00205 and RR42_RS00210 overlap by 4 nucleotides
RR42_RS00195: RR42_RS00195 - ABC transporter permease, at 64,311 to 65,240
_RS00195
RR42_RS00200: RR42_RS00200 - ABC transporter permease, at 65,299 to 66,429
_RS00200
RR42_RS00205: RR42_RS00205 - branched-chain amino acid ABC transporter ATP-binding protein, at 66,426 to 67,211
_RS00205
RR42_RS00210: RR42_RS00210 - ABC transporter ATP-binding protein, at 67,208 to 67,933
_RS00210
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1
2 at 64.329 kb on + strand at 64.330 kb on - strand at 64.363 kb on - strand at 64.385 kb on + strand at 64.385 kb on + strand at 64.472 kb on + strand, within RR42_RS00195 at 64.616 kb on + strand, within RR42_RS00195 at 64.616 kb on + strand, within RR42_RS00195 at 64.617 kb on - strand, within RR42_RS00195 at 64.823 kb on + strand, within RR42_RS00195 at 64.972 kb on - strand, within RR42_RS00195 at 65.149 kb on - strand at 65.335 kb on + strand at 65.335 kb on + strand at 65.336 kb on - strand at 65.524 kb on + strand, within RR42_RS00200 at 65.569 kb on + strand, within RR42_RS00200 at 65.569 kb on + strand, within RR42_RS00200 at 65.656 kb on - strand, within RR42_RS00200 at 65.656 kb on - strand, within RR42_RS00200 at 65.659 kb on + strand, within RR42_RS00200 at 65.660 kb on - strand, within RR42_RS00200 at 65.660 kb on - strand, within RR42_RS00200 at 65.785 kb on + strand, within RR42_RS00200 at 65.786 kb on - strand, within RR42_RS00200 at 65.900 kb on - strand, within RR42_RS00200 at 65.908 kb on - strand, within RR42_RS00200 at 65.956 kb on + strand, within RR42_RS00200 at 65.957 kb on - strand, within RR42_RS00200 at 65.957 kb on - strand, within RR42_RS00200 at 66.087 kb on + strand, within RR42_RS00200 at 66.154 kb on + strand, within RR42_RS00200 at 66.154 kb on + strand, within RR42_RS00200 at 66.591 kb on + strand, within RR42_RS00205 at 66.619 kb on - strand, within RR42_RS00205 at 66.661 kb on - strand, within RR42_RS00205 at 66.661 kb on - strand, within RR42_RS00205 at 66.816 kb on + strand, within RR42_RS00205 at 66.817 kb on - strand, within RR42_RS00205 at 66.830 kb on - strand, within RR42_RS00205 at 66.845 kb on + strand, within RR42_RS00205 at 67.122 kb on + strand, within RR42_RS00205 at 67.122 kb on + strand, within RR42_RS00205 at 67.122 kb on + strand, within RR42_RS00205 at 67.122 kb on + strand, within RR42_RS00205 at 67.123 kb on - strand, within RR42_RS00205 at 67.123 kb on - strand, within RR42_RS00205 at 67.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.001 mM remove 64,329 + +0.7 64,330 - +0.6 64,363 - +0.7 64,385 + -0.1 64,385 + +0.0 64,472 + RR42_RS00195 0.17 +0.5 64,616 + RR42_RS00195 0.33 -0.3 64,616 + RR42_RS00195 0.33 +0.3 64,617 - RR42_RS00195 0.33 +0.7 64,823 + RR42_RS00195 0.55 +1.1 64,972 - RR42_RS00195 0.71 -0.1 65,149 - -0.3 65,335 + +2.3 65,335 + +2.3 65,336 - +0.1 65,524 + RR42_RS00200 0.20 +1.5 65,569 + RR42_RS00200 0.24 -0.1 65,569 + RR42_RS00200 0.24 -1.3 65,656 - RR42_RS00200 0.32 +0.0 65,656 - RR42_RS00200 0.32 -0.5 65,659 + RR42_RS00200 0.32 +1.0 65,660 - RR42_RS00200 0.32 +0.1 65,660 - RR42_RS00200 0.32 -0.9 65,785 + RR42_RS00200 0.43 -1.3 65,786 - RR42_RS00200 0.43 +0.5 65,900 - RR42_RS00200 0.53 -1.6 65,908 - RR42_RS00200 0.54 +1.2 65,956 + RR42_RS00200 0.58 -0.2 65,957 - RR42_RS00200 0.58 +0.1 65,957 - RR42_RS00200 0.58 -0.6 66,087 + RR42_RS00200 0.70 +0.2 66,154 + RR42_RS00200 0.76 -0.9 66,154 + RR42_RS00200 0.76 -0.4 66,591 + RR42_RS00205 0.21 -0.9 66,619 - RR42_RS00205 0.25 +0.5 66,661 - RR42_RS00205 0.30 +0.7 66,661 - RR42_RS00205 0.30 +1.6 66,816 + RR42_RS00205 0.50 +0.5 66,817 - RR42_RS00205 0.50 -0.5 66,830 - RR42_RS00205 0.51 -0.3 66,845 + RR42_RS00205 0.53 +1.5 67,122 + RR42_RS00205 0.89 -0.3 67,122 + RR42_RS00205 0.89 +0.3 67,122 + RR42_RS00205 0.89 +1.6 67,122 + RR42_RS00205 0.89 +0.5 67,123 - RR42_RS00205 0.89 +0.6 67,123 - RR42_RS00205 0.89 -1.6 67,189 - -0.0
Or see this region's nucleotide sequence