Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4864

Experiment: Thymine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4863 and Pf1N1B4_4864 overlap by 4 nucleotidesPf1N1B4_4864 and Pf1N1B4_4865 are separated by 12 nucleotides Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766 _4863 Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954 _4864 Pf1N1B4_4865 - Multidrug efflux membrane fusion protein MexE, at 5,382,967 to 5,384,220 _4865 Position (kb) 5379 5380 5381 5382 5383Strain fitness (log2 ratio) -2 -1 0 1 2at 5379.232 kb on - strand, within Pf1N1B4_4863at 5379.379 kb on - strand, within Pf1N1B4_4863at 5379.851 kb on - strandat 5379.851 kb on - strandat 5379.854 kb on + strandat 5379.854 kb on + strandat 5379.855 kb on - strandat 5379.855 kb on - strandat 5379.855 kb on - strandat 5379.855 kb on - strandat 5379.855 kb on - strandat 5379.855 kb on - strandat 5379.855 kb on - strandat 5380.110 kb on + strand, within Pf1N1B4_4864at 5380.111 kb on - strand, within Pf1N1B4_4864at 5380.111 kb on - strandat 5380.254 kb on - strand, within Pf1N1B4_4864at 5380.286 kb on - strand, within Pf1N1B4_4864at 5380.337 kb on + strandat 5380.338 kb on - strand, within Pf1N1B4_4864at 5380.476 kb on - strand, within Pf1N1B4_4864at 5380.476 kb on - strand, within Pf1N1B4_4864at 5380.476 kb on - strand, within Pf1N1B4_4864at 5380.476 kb on - strand, within Pf1N1B4_4864at 5380.625 kb on + strand, within Pf1N1B4_4864at 5380.625 kb on + strand, within Pf1N1B4_4864at 5380.658 kb on + strand, within Pf1N1B4_4864at 5380.673 kb on + strand, within Pf1N1B4_4864at 5380.772 kb on + strand, within Pf1N1B4_4864at 5380.773 kb on - strand, within Pf1N1B4_4864at 5380.773 kb on - strand, within Pf1N1B4_4864at 5380.841 kb on + strand, within Pf1N1B4_4864at 5380.842 kb on - strand, within Pf1N1B4_4864at 5380.943 kb on + strand, within Pf1N1B4_4864at 5380.943 kb on + strand, within Pf1N1B4_4864at 5380.944 kb on - strand, within Pf1N1B4_4864at 5380.944 kb on - strand, within Pf1N1B4_4864at 5381.149 kb on - strand, within Pf1N1B4_4864at 5381.149 kb on - strand, within Pf1N1B4_4864at 5381.149 kb on - strand, within Pf1N1B4_4864at 5381.217 kb on - strand, within Pf1N1B4_4864at 5381.336 kb on - strand, within Pf1N1B4_4864at 5381.349 kb on - strand, within Pf1N1B4_4864at 5381.349 kb on - strand, within Pf1N1B4_4864at 5381.349 kb on - strandat 5381.548 kb on - strand, within Pf1N1B4_4864at 5381.549 kb on + strandat 5381.890 kb on + strand, within Pf1N1B4_4864at 5381.966 kb on + strand, within Pf1N1B4_4864at 5381.966 kb on + strand, within Pf1N1B4_4864at 5382.196 kb on - strand, within Pf1N1B4_4864at 5382.479 kb on + strand, within Pf1N1B4_4864at 5382.480 kb on - strandat 5382.480 kb on - strand, within Pf1N1B4_4864at 5382.491 kb on + strand, within Pf1N1B4_4864at 5382.492 kb on - strand, within Pf1N1B4_4864at 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.720 kb on - strandat 5382.720 kb on - strandat 5382.957 kb on + strandat 5382.957 kb on + strandat 5383.331 kb on + strand, within Pf1N1B4_4865at 5383.554 kb on - strand, within Pf1N1B4_4865at 5383.557 kb on + strand, within Pf1N1B4_4865at 5383.558 kb on - strand, within Pf1N1B4_4865at 5383.558 kb on - strand, within Pf1N1B4_4865at 5383.689 kb on + strand, within Pf1N1B4_4865

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Per-strain Table

Position Strand Gene LocusTag Fraction Thymine (N)
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5,379,232 - Pf1N1B4_4863 0.62 +0.5
5,379,379 - Pf1N1B4_4863 0.73 +1.1
5,379,851 - -1.2
5,379,851 - +1.5
5,379,854 + -2.1
5,379,854 + +0.8
5,379,855 - +1.1
5,379,855 - +0.5
5,379,855 - -0.0
5,379,855 - +0.8
5,379,855 - +0.1
5,379,855 - +1.2
5,379,855 - +0.7
5,380,110 + Pf1N1B4_4864 0.11 +0.1
5,380,111 - Pf1N1B4_4864 0.11 +1.1
5,380,111 - -0.5
5,380,254 - Pf1N1B4_4864 0.15 -0.5
5,380,286 - Pf1N1B4_4864 0.16 -1.5
5,380,337 + -0.5
5,380,338 - Pf1N1B4_4864 0.18 +0.5
5,380,476 - Pf1N1B4_4864 0.22 -1.5
5,380,476 - Pf1N1B4_4864 0.22 -1.1
5,380,476 - Pf1N1B4_4864 0.22 +0.7
5,380,476 - Pf1N1B4_4864 0.22 -0.1
5,380,625 + Pf1N1B4_4864 0.27 -2.1
5,380,625 + Pf1N1B4_4864 0.27 +1.8
5,380,658 + Pf1N1B4_4864 0.28 -0.5
5,380,673 + Pf1N1B4_4864 0.29 +0.1
5,380,772 + Pf1N1B4_4864 0.32 +0.3
5,380,773 - Pf1N1B4_4864 0.32 +0.7
5,380,773 - Pf1N1B4_4864 0.32 +0.9
5,380,841 + Pf1N1B4_4864 0.34 +0.8
5,380,842 - Pf1N1B4_4864 0.34 -0.1
5,380,943 + Pf1N1B4_4864 0.37 +1.8
5,380,943 + Pf1N1B4_4864 0.37 -1.1
5,380,944 - Pf1N1B4_4864 0.37 +1.5
5,380,944 - Pf1N1B4_4864 0.37 +0.5
5,381,149 - Pf1N1B4_4864 0.43 -0.5
5,381,149 - Pf1N1B4_4864 0.43 +1.1
5,381,149 - Pf1N1B4_4864 0.43 +1.5
5,381,217 - Pf1N1B4_4864 0.46 -0.5
5,381,336 - Pf1N1B4_4864 0.49 +0.1
5,381,349 - Pf1N1B4_4864 0.50 +1.5
5,381,349 - Pf1N1B4_4864 0.50 +1.1
5,381,349 - +0.5
5,381,548 - Pf1N1B4_4864 0.56 +1.3
5,381,549 + +0.5
5,381,890 + Pf1N1B4_4864 0.67 -0.5
5,381,966 + Pf1N1B4_4864 0.69 -1.8
5,381,966 + Pf1N1B4_4864 0.69 -1.5
5,382,196 - Pf1N1B4_4864 0.76 +1.1
5,382,479 + Pf1N1B4_4864 0.85 +0.5
5,382,480 - -0.5
5,382,480 - Pf1N1B4_4864 0.85 -0.1
5,382,491 + Pf1N1B4_4864 0.85 +0.1
5,382,492 - Pf1N1B4_4864 0.85 +0.1
5,382,719 + +0.5
5,382,719 + +0.1
5,382,719 + -1.1
5,382,719 + -0.5
5,382,719 + +0.5
5,382,720 - +1.5
5,382,720 - +0.5
5,382,957 + +0.5
5,382,957 + +1.8
5,383,331 + Pf1N1B4_4865 0.29 +0.1
5,383,554 - Pf1N1B4_4865 0.47 -1.5
5,383,557 + Pf1N1B4_4865 0.47 +0.6
5,383,558 - Pf1N1B4_4865 0.47 +1.1
5,383,558 - Pf1N1B4_4865 0.47 -1.1
5,383,689 + Pf1N1B4_4865 0.58 +0.5

Or see this region's nucleotide sequence