Experiment: ALP with sodium fluoride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2008 and Dsui_2009 are separated by 455 nucleotides Dsui_2009 and Dsui_2010 are separated by 192 nucleotides
Dsui_2008: Dsui_2008 - tetratricopeptide repeat protein, at 2,117,219 to 2,117,806
_2008
Dsui_2009: Dsui_2009 - tRNA-Ser, at 2,118,262 to 2,118,352
_2009
Dsui_2010: Dsui_2010 - micrococcal nuclease-like nuclease, at 2,118,545 to 2,119,039
_2010
Position (kb)
2118
2119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2117.304 kb on - strand at 2117.349 kb on + strand, within Dsui_2008 at 2117.462 kb on + strand, within Dsui_2008 at 2117.486 kb on - strand, within Dsui_2008 at 2117.502 kb on - strand, within Dsui_2008 at 2117.517 kb on + strand, within Dsui_2008 at 2117.641 kb on + strand, within Dsui_2008 at 2117.648 kb on - strand, within Dsui_2008 at 2117.695 kb on + strand at 2117.703 kb on - strand, within Dsui_2008 at 2117.715 kb on + strand, within Dsui_2008 at 2117.730 kb on + strand, within Dsui_2008 at 2117.774 kb on + strand at 2117.826 kb on - strand at 2117.828 kb on + strand at 2117.836 kb on - strand at 2118.095 kb on + strand at 2118.119 kb on - strand at 2118.244 kb on + strand at 2118.388 kb on + strand at 2118.507 kb on + strand at 2118.513 kb on + strand at 2118.513 kb on + strand at 2118.513 kb on + strand at 2118.513 kb on + strand at 2118.513 kb on + strand at 2118.513 kb on + strand at 2118.521 kb on - strand at 2118.521 kb on - strand at 2118.521 kb on - strand at 2118.521 kb on - strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.536 kb on + strand at 2118.544 kb on - strand at 2118.544 kb on - strand at 2118.544 kb on - strand at 2118.544 kb on - strand at 2118.544 kb on - strand at 2118.544 kb on - strand at 2118.544 kb on - strand at 2118.559 kb on + strand at 2118.560 kb on + strand at 2118.567 kb on - strand at 2118.568 kb on - strand at 2118.573 kb on + strand at 2118.638 kb on + strand, within Dsui_2010 at 2118.644 kb on + strand, within Dsui_2010 at 2118.646 kb on - strand at 2118.646 kb on - strand, within Dsui_2010 at 2118.666 kb on - strand at 2118.673 kb on - strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.753 kb on + strand, within Dsui_2010 at 2118.756 kb on - strand, within Dsui_2010 at 2118.761 kb on - strand, within Dsui_2010 at 2118.761 kb on - strand, within Dsui_2010 at 2118.761 kb on - strand, within Dsui_2010 at 2118.784 kb on + strand, within Dsui_2010 at 2118.806 kb on + strand, within Dsui_2010 at 2118.824 kb on + strand, within Dsui_2010 at 2118.824 kb on + strand, within Dsui_2010 at 2118.832 kb on - strand, within Dsui_2010 at 2118.832 kb on - strand, within Dsui_2010 at 2118.857 kb on + strand, within Dsui_2010 at 2118.863 kb on + strand, within Dsui_2010 at 2118.864 kb on + strand, within Dsui_2010 at 2118.903 kb on + strand, within Dsui_2010 at 2118.903 kb on + strand, within Dsui_2010 at 2118.903 kb on + strand, within Dsui_2010 at 2118.906 kb on - strand, within Dsui_2010 at 2118.921 kb on - strand, within Dsui_2010 at 2118.926 kb on + strand, within Dsui_2010 at 2118.926 kb on + strand, within Dsui_2010 at 2118.926 kb on + strand, within Dsui_2010 at 2118.926 kb on + strand, within Dsui_2010 at 2118.926 kb on + strand, within Dsui_2010 at 2118.934 kb on - strand, within Dsui_2010 at 2118.934 kb on - strand, within Dsui_2010 at 2118.934 kb on - strand, within Dsui_2010 at 2118.934 kb on - strand, within Dsui_2010 at 2119.017 kb on + strand at 2119.022 kb on + strand at 2119.025 kb on + strand at 2119.051 kb on - strand at 2119.056 kb on + strand at 2119.056 kb on + strand at 2119.063 kb on + strand at 2119.074 kb on + strand at 2119.082 kb on - strand at 2119.082 kb on - strand at 2119.082 kb on - strand at 2119.145 kb on + strand at 2119.165 kb on + strand at 2119.167 kb on + strand at 2119.223 kb on - strand at 2119.274 kb on + strand at 2119.327 kb on - strand at 2119.327 kb on - strand at 2119.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with sodium fluoride 40 mM remove 2,117,304 - +0.2 2,117,349 + Dsui_2008 0.22 -0.8 2,117,462 + Dsui_2008 0.41 -1.4 2,117,486 - Dsui_2008 0.45 +0.2 2,117,502 - Dsui_2008 0.48 +1.2 2,117,517 + Dsui_2008 0.51 +1.2 2,117,641 + Dsui_2008 0.72 +1.2 2,117,648 - Dsui_2008 0.73 +0.2 2,117,695 + +0.2 2,117,703 - Dsui_2008 0.82 +0.2 2,117,715 + Dsui_2008 0.84 -0.4 2,117,730 + Dsui_2008 0.87 +1.2 2,117,774 + +0.2 2,117,826 - -0.8 2,117,828 + -0.4 2,117,836 - +0.2 2,118,095 + +0.2 2,118,119 - +1.2 2,118,244 + +0.4 2,118,388 + +0.3 2,118,507 + +0.2 2,118,513 + +0.5 2,118,513 + -0.4 2,118,513 + -2.2 2,118,513 + +0.2 2,118,513 + +0.3 2,118,513 + +0.3 2,118,521 - +1.2 2,118,521 - +0.2 2,118,521 - -0.8 2,118,521 - -0.8 2,118,536 + -1.5 2,118,536 + +0.9 2,118,536 + -0.3 2,118,536 + +0.3 2,118,536 + -0.0 2,118,536 + +0.2 2,118,536 + -0.1 2,118,536 + -0.3 2,118,536 + -0.4 2,118,536 + -0.2 2,118,536 + -0.4 2,118,536 + +0.2 2,118,544 - -0.4 2,118,544 - +0.2 2,118,544 - +1.2 2,118,544 - +1.2 2,118,544 - +1.2 2,118,544 - -0.4 2,118,544 - -1.2 2,118,559 + -0.6 2,118,560 + -0.5 2,118,567 - -0.4 2,118,568 - -0.8 2,118,573 + +0.4 2,118,638 + Dsui_2010 0.19 -1.6 2,118,644 + Dsui_2010 0.20 +0.2 2,118,646 - +1.2 2,118,646 - Dsui_2010 0.20 +0.2 2,118,666 - +1.2 2,118,673 - Dsui_2010 0.26 +1.2 2,118,753 + Dsui_2010 0.42 +2.7 2,118,753 + Dsui_2010 0.42 +2.2 2,118,753 + Dsui_2010 0.42 +1.2 2,118,753 + Dsui_2010 0.42 +1.2 2,118,753 + Dsui_2010 0.42 -0.4 2,118,753 + Dsui_2010 0.42 +1.2 2,118,753 + Dsui_2010 0.42 +1.2 2,118,756 - Dsui_2010 0.43 -0.4 2,118,761 - Dsui_2010 0.44 +0.2 2,118,761 - Dsui_2010 0.44 -1.8 2,118,761 - Dsui_2010 0.44 -2.0 2,118,784 + Dsui_2010 0.48 +1.2 2,118,806 + Dsui_2010 0.53 +0.2 2,118,824 + Dsui_2010 0.56 -0.2 2,118,824 + Dsui_2010 0.56 +1.2 2,118,832 - Dsui_2010 0.58 +1.2 2,118,832 - Dsui_2010 0.58 +0.2 2,118,857 + Dsui_2010 0.63 -0.4 2,118,863 + Dsui_2010 0.64 +0.2 2,118,864 + Dsui_2010 0.64 -0.8 2,118,903 + Dsui_2010 0.72 +0.2 2,118,903 + Dsui_2010 0.72 +1.2 2,118,903 + Dsui_2010 0.72 +1.2 2,118,906 - Dsui_2010 0.73 -1.2 2,118,921 - Dsui_2010 0.76 +0.2 2,118,926 + Dsui_2010 0.77 +0.2 2,118,926 + Dsui_2010 0.77 -0.4 2,118,926 + Dsui_2010 0.77 +1.2 2,118,926 + Dsui_2010 0.77 +0.2 2,118,926 + Dsui_2010 0.77 +1.2 2,118,934 - Dsui_2010 0.79 +1.2 2,118,934 - Dsui_2010 0.79 +1.2 2,118,934 - Dsui_2010 0.79 -0.8 2,118,934 - Dsui_2010 0.79 +0.2 2,119,017 + +1.2 2,119,022 + -1.4 2,119,025 + +0.2 2,119,051 - +1.2 2,119,056 + +1.2 2,119,056 + -1.4 2,119,063 + +1.2 2,119,074 + -2.2 2,119,082 - +1.2 2,119,082 - -1.8 2,119,082 - +1.2 2,119,145 + +1.2 2,119,165 + -0.8 2,119,167 + +0.2 2,119,223 - -1.2 2,119,274 + +1.2 2,119,327 - -0.4 2,119,327 - +1.2 2,119,341 + +1.2
Or see this region's nucleotide sequence