Experiment: ALP with sodium fluoride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1868 and Dsui_1869 are separated by 322 nucleotides Dsui_1869 and Dsui_1870 are separated by 130 nucleotides Dsui_1870 and Dsui_1871 overlap by 4 nucleotides
Dsui_1868: Dsui_1868 - HD-GYP domain-containing protein, at 1,964,363 to 1,965,607
_1868
Dsui_1869: Dsui_1869 - hypothetical protein, at 1,965,930 to 1,966,208
_1869
Dsui_1870: Dsui_1870 - efflux transporter, outer membrane factor lipoprotein, NodT family, at 1,966,339 to 1,967,841
_1870
Dsui_1871: Dsui_1871 - hydrophobe/amphiphile efflux-1 (HAE1) family transporter, at 1,967,838 to 1,970,972
_1871
Position (kb)
1966
1967
1968 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1965.392 kb on + strand, within Dsui_1868 at 1965.443 kb on - strand at 1965.708 kb on - strand at 1965.712 kb on + strand at 1965.753 kb on - strand at 1965.872 kb on + strand at 1965.920 kb on + strand at 1965.944 kb on + strand at 1965.961 kb on + strand, within Dsui_1869 at 1966.004 kb on + strand, within Dsui_1869 at 1966.083 kb on + strand, within Dsui_1869 at 1966.113 kb on + strand, within Dsui_1869 at 1966.349 kb on + strand at 1966.349 kb on + strand at 1966.432 kb on - strand at 1966.436 kb on + strand at 1966.459 kb on + strand at 1966.468 kb on + strand at 1966.494 kb on + strand, within Dsui_1870 at 1966.564 kb on + strand, within Dsui_1870 at 1966.686 kb on - strand, within Dsui_1870 at 1966.724 kb on + strand, within Dsui_1870 at 1966.724 kb on + strand at 1966.724 kb on + strand, within Dsui_1870 at 1966.732 kb on - strand, within Dsui_1870 at 1966.732 kb on - strand, within Dsui_1870 at 1966.732 kb on - strand, within Dsui_1870 at 1966.734 kb on + strand, within Dsui_1870 at 1966.872 kb on + strand, within Dsui_1870 at 1966.872 kb on + strand, within Dsui_1870 at 1966.880 kb on - strand, within Dsui_1870 at 1966.884 kb on - strand, within Dsui_1870 at 1967.100 kb on + strand, within Dsui_1870 at 1967.116 kb on - strand, within Dsui_1870 at 1967.197 kb on + strand, within Dsui_1870 at 1967.205 kb on - strand, within Dsui_1870 at 1967.265 kb on + strand, within Dsui_1870 at 1967.265 kb on + strand, within Dsui_1870 at 1967.273 kb on - strand, within Dsui_1870 at 1967.273 kb on - strand, within Dsui_1870 at 1967.465 kb on + strand at 1967.492 kb on + strand, within Dsui_1870 at 1967.557 kb on - strand at 1967.567 kb on - strand, within Dsui_1870 at 1967.584 kb on - strand, within Dsui_1870 at 1967.770 kb on + strand at 1967.778 kb on - strand at 1967.811 kb on - strand at 1967.862 kb on - strand at 1967.964 kb on - strand at 1968.141 kb on + strand at 1968.156 kb on - strand, within Dsui_1871 at 1968.353 kb on - strand, within Dsui_1871 at 1968.416 kb on - strand, within Dsui_1871 at 1968.446 kb on + strand, within Dsui_1871 at 1968.490 kb on + strand at 1968.490 kb on + strand, within Dsui_1871 at 1968.522 kb on - strand, within Dsui_1871 at 1968.557 kb on + strand at 1968.565 kb on - strand, within Dsui_1871 at 1968.580 kb on + strand, within Dsui_1871 at 1968.593 kb on - strand, within Dsui_1871 at 1968.613 kb on - strand, within Dsui_1871 at 1968.613 kb on - strand, within Dsui_1871 at 1968.628 kb on + strand, within Dsui_1871 at 1968.629 kb on + strand at 1968.676 kb on + strand, within Dsui_1871 at 1968.725 kb on + strand, within Dsui_1871 at 1968.732 kb on - strand, within Dsui_1871 at 1968.795 kb on - strand, within Dsui_1871 at 1968.824 kb on + strand, within Dsui_1871
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with sodium fluoride 40 mM remove 1,965,392 + Dsui_1868 0.83 -0.4 1,965,443 - -1.1 1,965,708 - -0.1 1,965,712 + -0.1 1,965,753 - +0.9 1,965,872 + -0.0 1,965,920 + -0.0 1,965,944 + +0.1 1,965,961 + Dsui_1869 0.11 +0.2 1,966,004 + Dsui_1869 0.27 +0.2 1,966,083 + Dsui_1869 0.55 +0.0 1,966,113 + Dsui_1869 0.66 +0.1 1,966,349 + -0.4 1,966,349 + +0.0 1,966,432 - +0.9 1,966,436 + -0.1 1,966,459 + -1.2 1,966,468 + -1.4 1,966,494 + Dsui_1870 0.10 +0.7 1,966,564 + Dsui_1870 0.15 -0.7 1,966,686 - Dsui_1870 0.23 -0.7 1,966,724 + Dsui_1870 0.26 -0.7 1,966,724 + +0.9 1,966,724 + Dsui_1870 0.26 -0.1 1,966,732 - Dsui_1870 0.26 +0.9 1,966,732 - Dsui_1870 0.26 -1.1 1,966,732 - Dsui_1870 0.26 -2.6 1,966,734 + Dsui_1870 0.26 -1.7 1,966,872 + Dsui_1870 0.35 -0.1 1,966,872 + Dsui_1870 0.35 -1.4 1,966,880 - Dsui_1870 0.36 -1.6 1,966,884 - Dsui_1870 0.36 +1.5 1,967,100 + Dsui_1870 0.51 -1.4 1,967,116 - Dsui_1870 0.52 -0.9 1,967,197 + Dsui_1870 0.57 +0.9 1,967,205 - Dsui_1870 0.58 -0.4 1,967,265 + Dsui_1870 0.62 -0.1 1,967,265 + Dsui_1870 0.62 -0.7 1,967,273 - Dsui_1870 0.62 +0.1 1,967,273 - Dsui_1870 0.62 -1.2 1,967,465 + +0.9 1,967,492 + Dsui_1870 0.77 -1.1 1,967,557 - -0.1 1,967,567 - Dsui_1870 0.82 -0.1 1,967,584 - Dsui_1870 0.83 +0.9 1,967,770 + +0.9 1,967,778 - -0.1 1,967,811 - +1.2 1,967,862 - +0.9 1,967,964 - +0.9 1,968,141 + +0.9 1,968,156 - Dsui_1871 0.10 +0.9 1,968,353 - Dsui_1871 0.16 -0.1 1,968,416 - Dsui_1871 0.18 -0.8 1,968,446 + Dsui_1871 0.19 -0.1 1,968,490 + +0.9 1,968,490 + Dsui_1871 0.21 -1.1 1,968,522 - Dsui_1871 0.22 +0.1 1,968,557 + -0.1 1,968,565 - Dsui_1871 0.23 -0.4 1,968,580 + Dsui_1871 0.24 -0.7 1,968,593 - Dsui_1871 0.24 -1.0 1,968,613 - Dsui_1871 0.25 +0.3 1,968,613 - Dsui_1871 0.25 -1.1 1,968,628 + Dsui_1871 0.25 -0.1 1,968,629 + +0.9 1,968,676 + Dsui_1871 0.27 +0.9 1,968,725 + Dsui_1871 0.28 +1.9 1,968,732 - Dsui_1871 0.29 -0.4 1,968,795 - Dsui_1871 0.31 -0.1 1,968,824 + Dsui_1871 0.31 -0.1
Or see this region's nucleotide sequence