Experiment: ALP with sodium fluoride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1209 and Dsui_1210 are separated by 80 nucleotides Dsui_1210 and Dsui_1211 are separated by 40 nucleotides Dsui_1211 and Dsui_1212 are separated by 93 nucleotides Dsui_1212 and Dsui_1213 are separated by 113 nucleotides
Dsui_1209: Dsui_1209 - RNA polymerase sigma factor, sigma-70 family, at 1,265,765 to 1,267,735
_1209
Dsui_1210: Dsui_1210 - DNA primase, catalytic core, at 1,267,816 to 1,269,570
_1210
Dsui_1211: Dsui_1211 - hypothetical protein, at 1,269,611 to 1,270,057
_1211
Dsui_1212: Dsui_1212 - ribosomal protein S21, at 1,270,151 to 1,270,363
_1212
Dsui_1213: Dsui_1213 - putative glycoprotease GCP, at 1,270,477 to 1,271,505
_1213
Position (kb)
1267
1268
1269
1270 Strain fitness (log2 ratio)
-1
0
1 at 1269.627 kb on + strand at 1269.634 kb on - strand at 1269.686 kb on - strand, within Dsui_1211 at 1269.686 kb on - strand, within Dsui_1211 at 1269.686 kb on - strand, within Dsui_1211 at 1269.741 kb on + strand, within Dsui_1211 at 1269.749 kb on - strand, within Dsui_1211 at 1270.028 kb on - strand at 1270.060 kb on - strand at 1270.148 kb on - strand at 1270.461 kb on + strand at 1270.469 kb on - strand at 1270.469 kb on - strand at 1270.469 kb on - strand at 1270.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with sodium fluoride 40 mM remove 1,269,627 + +0.6 1,269,634 - -0.5 1,269,686 - Dsui_1211 0.17 +0.2 1,269,686 - Dsui_1211 0.17 -0.2 1,269,686 - Dsui_1211 0.17 -0.2 1,269,741 + Dsui_1211 0.29 -0.4 1,269,749 - Dsui_1211 0.31 -0.3 1,270,028 - -1.2 1,270,060 - -1.3 1,270,148 - -0.6 1,270,461 + -0.1 1,270,469 - +0.6 1,270,469 - +0.6 1,270,469 - +0.6 1,270,469 - +0.6
Or see this region's nucleotide sequence