Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS36940

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS36925 and RR42_RS36930 are separated by 82 nucleotidesRR42_RS36930 and RR42_RS36935 are separated by 92 nucleotidesRR42_RS36935 and RR42_RS36940 overlap by 17 nucleotidesRR42_RS36940 and RR42_RS36945 are separated by 157 nucleotides RR42_RS36925: RR42_RS36925 - carotenoid oxygenase, at 3,739,201 to 3,740,595 _RS36925 RR42_RS36930: RR42_RS36930 - membrane protein, at 3,740,678 to 3,741,265 _RS36930 RR42_RS36935: RR42_RS36935 - hypothetical protein, at 3,741,358 to 3,741,579 _RS36935 RR42_RS36940: RR42_RS36940 - alcohol dehydrogenase, at 3,741,563 to 3,742,651 _RS36940 RR42_RS36945: RR42_RS36945 - mechanosensitive ion channel protein MscS, at 3,742,809 to 3,743,666 _RS36945 Position (kb) 3741 3742 3743Strain fitness (log2 ratio) -2 -1 0 1 2at 3740.685 kb on + strandat 3740.686 kb on - strandat 3740.867 kb on + strand, within RR42_RS36930at 3740.867 kb on + strand, within RR42_RS36930at 3740.868 kb on - strand, within RR42_RS36930at 3740.868 kb on - strand, within RR42_RS36930at 3740.870 kb on - strand, within RR42_RS36930at 3740.984 kb on + strand, within RR42_RS36930at 3740.984 kb on + strand, within RR42_RS36930at 3740.985 kb on - strand, within RR42_RS36930at 3741.011 kb on + strand, within RR42_RS36930at 3741.049 kb on + strand, within RR42_RS36930at 3741.049 kb on + strand, within RR42_RS36930at 3741.050 kb on - strand, within RR42_RS36930at 3741.050 kb on - strand, within RR42_RS36930at 3741.050 kb on - strand, within RR42_RS36930at 3741.050 kb on - strand, within RR42_RS36930at 3741.088 kb on - strand, within RR42_RS36930at 3741.110 kb on + strand, within RR42_RS36930at 3741.263 kb on + strandat 3741.272 kb on - strandat 3741.448 kb on + strand, within RR42_RS36935at 3741.448 kb on + strand, within RR42_RS36935at 3741.449 kb on - strand, within RR42_RS36935at 3741.546 kb on + strand, within RR42_RS36935at 3741.546 kb on + strand, within RR42_RS36935at 3741.546 kb on + strand, within RR42_RS36935at 3741.546 kb on + strand, within RR42_RS36935at 3741.884 kb on + strand, within RR42_RS36940at 3741.884 kb on + strand, within RR42_RS36940at 3741.885 kb on - strand, within RR42_RS36940at 3742.728 kb on - strandat 3742.732 kb on + strandat 3742.732 kb on + strandat 3742.732 kb on + strandat 3742.732 kb on + strandat 3742.733 kb on - strandat 3742.733 kb on - strandat 3742.733 kb on - strandat 3742.733 kb on - strandat 3742.733 kb on - strandat 3742.733 kb on - strandat 3742.762 kb on - strandat 3742.762 kb on - strandat 3742.873 kb on + strandat 3742.874 kb on - strandat 3743.021 kb on - strand, within RR42_RS36945at 3743.152 kb on + strand, within RR42_RS36945at 3743.152 kb on + strand, within RR42_RS36945at 3743.152 kb on + strand, within RR42_RS36945at 3743.153 kb on - strand, within RR42_RS36945at 3743.153 kb on - strand, within RR42_RS36945at 3743.153 kb on - strand, within RR42_RS36945at 3743.153 kb on - strand, within RR42_RS36945at 3743.621 kb on + strandat 3743.634 kb on + strandat 3743.635 kb on - strandat 3743.635 kb on - strandat 3743.635 kb on - strandat 3743.641 kb on + strandat 3743.642 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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3,740,685 + -1.5
3,740,686 - -0.1
3,740,867 + RR42_RS36930 0.32 +0.8
3,740,867 + RR42_RS36930 0.32 -1.0
3,740,868 - RR42_RS36930 0.32 +1.0
3,740,868 - RR42_RS36930 0.32 +0.4
3,740,870 - RR42_RS36930 0.33 -0.7
3,740,984 + RR42_RS36930 0.52 +0.1
3,740,984 + RR42_RS36930 0.52 -0.0
3,740,985 - RR42_RS36930 0.52 -0.3
3,741,011 + RR42_RS36930 0.57 -1.7
3,741,049 + RR42_RS36930 0.63 +0.1
3,741,049 + RR42_RS36930 0.63 +0.5
3,741,050 - RR42_RS36930 0.63 -0.5
3,741,050 - RR42_RS36930 0.63 +0.4
3,741,050 - RR42_RS36930 0.63 +2.3
3,741,050 - RR42_RS36930 0.63 +0.2
3,741,088 - RR42_RS36930 0.70 -1.0
3,741,110 + RR42_RS36930 0.73 +0.6
3,741,263 + -0.5
3,741,272 - +0.5
3,741,448 + RR42_RS36935 0.41 -0.7
3,741,448 + RR42_RS36935 0.41 -0.5
3,741,449 - RR42_RS36935 0.41 +1.4
3,741,546 + RR42_RS36935 0.85 -2.2
3,741,546 + RR42_RS36935 0.85 +0.6
3,741,546 + RR42_RS36935 0.85 +0.9
3,741,546 + RR42_RS36935 0.85 +0.2
3,741,884 + RR42_RS36940 0.29 -1.2
3,741,884 + RR42_RS36940 0.29 -0.3
3,741,885 - RR42_RS36940 0.30 -1.2
3,742,728 - -0.9
3,742,732 + -0.8
3,742,732 + -0.2
3,742,732 + -2.7
3,742,732 + -1.3
3,742,733 - -0.1
3,742,733 - -0.2
3,742,733 - +0.1
3,742,733 - -0.7
3,742,733 - +1.7
3,742,733 - +0.8
3,742,762 - +0.0
3,742,762 - -0.4
3,742,873 + -0.5
3,742,874 - +0.2
3,743,021 - RR42_RS36945 0.25 -2.2
3,743,152 + RR42_RS36945 0.40 -0.5
3,743,152 + RR42_RS36945 0.40 -1.3
3,743,152 + RR42_RS36945 0.40 -0.3
3,743,153 - RR42_RS36945 0.40 +0.6
3,743,153 - RR42_RS36945 0.40 -0.9
3,743,153 - RR42_RS36945 0.40 -0.9
3,743,153 - RR42_RS36945 0.40 +0.1
3,743,621 + -0.4
3,743,634 + -1.4
3,743,635 - -0.1
3,743,635 - -0.1
3,743,635 - +0.4
3,743,641 + +0.3
3,743,642 - -0.0

Or see this region's nucleotide sequence