Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32235 and RR42_RS32240 are separated by 235 nucleotides RR42_RS32240 and RR42_RS32245 are separated by 281 nucleotides
RR42_RS32235: RR42_RS32235 - hopanoid biosynthesis associated radical SAM protein HpnH, at 2,618,430 to 2,619,596
_RS32235
RR42_RS32240: RR42_RS32240 - ABC transporter, at 2,619,832 to 2,620,479
_RS32240
RR42_RS32245: RR42_RS32245 - RND transporter, at 2,620,761 to 2,623,430
_RS32245
Position (kb)
2619
2620
2621 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2618.872 kb on - strand, within RR42_RS32235 at 2619.030 kb on + strand, within RR42_RS32235 at 2619.186 kb on + strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.265 kb on - strand, within RR42_RS32235 at 2619.312 kb on + strand, within RR42_RS32235 at 2619.313 kb on - strand, within RR42_RS32235 at 2619.357 kb on - strand, within RR42_RS32235 at 2619.438 kb on - strand, within RR42_RS32235 at 2619.462 kb on - strand, within RR42_RS32235 at 2619.492 kb on + strand at 2619.493 kb on - strand at 2619.493 kb on - strand at 2619.759 kb on - strand at 2619.872 kb on + strand at 2619.873 kb on - strand at 2619.873 kb on - strand at 2619.873 kb on - strand at 2619.909 kb on + strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.969 kb on - strand, within RR42_RS32240 at 2619.969 kb on - strand, within RR42_RS32240 at 2620.092 kb on - strand, within RR42_RS32240 at 2620.092 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.172 kb on + strand, within RR42_RS32240 at 2620.173 kb on - strand, within RR42_RS32240 at 2620.618 kb on + strand at 2620.619 kb on - strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.621 kb on - strand at 2620.621 kb on - strand at 2620.671 kb on - strand at 2620.673 kb on - strand at 2620.673 kb on - strand at 2620.673 kb on - strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.860 kb on + strand at 2620.860 kb on + strand at 2620.861 kb on - strand at 2620.861 kb on - strand at 2620.861 kb on - strand at 2620.861 kb on - strand at 2620.893 kb on - strand at 2621.124 kb on + strand, within RR42_RS32245 at 2621.229 kb on - strand, within RR42_RS32245 at 2621.442 kb on + strand, within RR42_RS32245 at 2621.442 kb on + strand, within RR42_RS32245 at 2621.442 kb on + strand, within RR42_RS32245 at 2621.443 kb on - strand, within RR42_RS32245 at 2621.443 kb on - strand, within RR42_RS32245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 2,618,872 - RR42_RS32235 0.38 -1.8 2,619,030 + RR42_RS32235 0.51 +0.2 2,619,186 + RR42_RS32235 0.65 -1.3 2,619,187 - RR42_RS32235 0.65 -2.2 2,619,187 - RR42_RS32235 0.65 -3.2 2,619,246 + RR42_RS32235 0.70 -4.2 2,619,246 + RR42_RS32235 0.70 -2.5 2,619,265 - RR42_RS32235 0.72 -4.0 2,619,312 + RR42_RS32235 0.76 -2.8 2,619,313 - RR42_RS32235 0.76 -1.1 2,619,357 - RR42_RS32235 0.79 -2.1 2,619,438 - RR42_RS32235 0.86 -1.3 2,619,462 - RR42_RS32235 0.88 -1.3 2,619,492 + -3.3 2,619,493 - -3.3 2,619,493 - -2.3 2,619,759 - -3.1 2,619,872 + -3.1 2,619,873 - -2.4 2,619,873 - -2.8 2,619,873 - -4.2 2,619,909 + RR42_RS32240 0.12 -2.5 2,619,930 - RR42_RS32240 0.15 -1.1 2,619,930 - RR42_RS32240 0.15 -2.3 2,619,930 - RR42_RS32240 0.15 -3.5 2,619,930 - RR42_RS32240 0.15 -0.8 2,619,968 + RR42_RS32240 0.21 -2.3 2,619,968 + RR42_RS32240 0.21 -4.0 2,619,968 + RR42_RS32240 0.21 -0.6 2,619,968 + RR42_RS32240 0.21 -1.7 2,619,969 - RR42_RS32240 0.21 -2.6 2,619,969 - RR42_RS32240 0.21 -4.3 2,620,092 - RR42_RS32240 0.40 -0.8 2,620,092 - RR42_RS32240 0.40 -5.1 2,620,140 - RR42_RS32240 0.48 -3.0 2,620,140 - RR42_RS32240 0.48 -4.8 2,620,140 - RR42_RS32240 0.48 -2.7 2,620,140 - RR42_RS32240 0.48 -5.4 2,620,140 - RR42_RS32240 0.48 -0.0 2,620,172 + RR42_RS32240 0.52 -2.6 2,620,173 - RR42_RS32240 0.53 -1.9 2,620,618 + -3.8 2,620,619 - -2.2 2,620,620 + -0.2 2,620,620 + -0.7 2,620,620 + -1.7 2,620,620 + -3.0 2,620,620 + -0.7 2,620,621 - +1.2 2,620,621 - -1.0 2,620,671 - +0.2 2,620,673 - -4.7 2,620,673 - -2.8 2,620,673 - -3.6 2,620,751 + -1.1 2,620,751 + -1.1 2,620,751 + -0.4 2,620,751 + -1.4 2,620,751 + -0.8 2,620,751 + -2.5 2,620,752 - -3.0 2,620,752 - -3.9 2,620,752 - -2.3 2,620,752 - -4.3 2,620,752 - -4.2 2,620,752 - -2.9 2,620,752 - -2.8 2,620,752 - -2.8 2,620,752 - -3.2 2,620,752 - -2.3 2,620,860 + -4.0 2,620,860 + -3.1 2,620,861 - -2.3 2,620,861 - -0.3 2,620,861 - -5.3 2,620,861 - -2.2 2,620,893 - +1.2 2,621,124 + RR42_RS32245 0.14 -3.7 2,621,229 - RR42_RS32245 0.18 -3.8 2,621,442 + RR42_RS32245 0.26 -4.2 2,621,442 + RR42_RS32245 0.26 -1.4 2,621,442 + RR42_RS32245 0.26 -1.9 2,621,443 - RR42_RS32245 0.26 -3.1 2,621,443 - RR42_RS32245 0.26 -2.1
Or see this region's nucleotide sequence