Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31075

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31070 and RR42_RS31075 are separated by 323 nucleotidesRR42_RS31075 and RR42_RS31080 are separated by 200 nucleotidesRR42_RS31080 and RR42_RS31085 are separated by 297 nucleotides RR42_RS31070: RR42_RS31070 - dihydroxy-acid dehydratase, at 2,350,732 to 2,352,462 _RS31070 RR42_RS31075: RR42_RS31075 - MFS transporter, at 2,352,786 to 2,353,814 _RS31075 RR42_RS31080: RR42_RS31080 - hypothetical protein, at 2,354,015 to 2,354,470 _RS31080 RR42_RS31085: RR42_RS31085 - hypothetical protein, at 2,354,768 to 2,355,184 _RS31085 Position (kb) 2352 2353 2354Strain fitness (log2 ratio) -2 -1 0 1at 2352.170 kb on + strand, within RR42_RS31070at 2352.170 kb on + strand, within RR42_RS31070at 2352.170 kb on + strand, within RR42_RS31070at 2352.170 kb on + strand, within RR42_RS31070at 2352.170 kb on + strand, within RR42_RS31070at 2352.170 kb on + strand, within RR42_RS31070at 2352.170 kb on + strand, within RR42_RS31070at 2352.171 kb on + strand, within RR42_RS31070at 2352.171 kb on - strand, within RR42_RS31070at 2352.171 kb on - strand, within RR42_RS31070at 2352.302 kb on + strandat 2352.303 kb on - strandat 2352.303 kb on - strandat 2352.360 kb on - strandat 2352.375 kb on + strandat 2352.376 kb on - strandat 2352.417 kb on - strandat 2352.417 kb on - strandat 2352.551 kb on - strandat 2352.685 kb on - strandat 2352.759 kb on + strandat 2352.760 kb on - strandat 2352.916 kb on - strand, within RR42_RS31075at 2353.098 kb on + strand, within RR42_RS31075at 2353.098 kb on + strand, within RR42_RS31075at 2353.098 kb on + strand, within RR42_RS31075at 2353.099 kb on - strand, within RR42_RS31075at 2353.100 kb on + strand, within RR42_RS31075at 2353.101 kb on - strand, within RR42_RS31075at 2353.149 kb on + strand, within RR42_RS31075at 2353.150 kb on - strand, within RR42_RS31075at 2353.167 kb on + strand, within RR42_RS31075at 2353.168 kb on - strand, within RR42_RS31075at 2353.392 kb on + strand, within RR42_RS31075at 2353.392 kb on + strand, within RR42_RS31075at 2353.393 kb on - strand, within RR42_RS31075at 2353.393 kb on - strand, within RR42_RS31075at 2353.476 kb on + strand, within RR42_RS31075at 2353.476 kb on + strand, within RR42_RS31075at 2353.477 kb on - strand, within RR42_RS31075at 2353.477 kb on - strand, within RR42_RS31075at 2353.480 kb on + strand, within RR42_RS31075at 2353.480 kb on + strand, within RR42_RS31075at 2353.481 kb on - strand, within RR42_RS31075at 2353.812 kb on + strandat 2353.812 kb on + strandat 2353.812 kb on + strandat 2353.812 kb on + strandat 2353.917 kb on + strandat 2353.927 kb on - strandat 2354.001 kb on + strandat 2354.001 kb on + strandat 2354.001 kb on + strandat 2354.030 kb on + strandat 2354.031 kb on - strandat 2354.031 kb on - strandat 2354.031 kb on - strandat 2354.031 kb on - strandat 2354.256 kb on - strand, within RR42_RS31080at 2354.256 kb on - strand, within RR42_RS31080at 2354.390 kb on + strand, within RR42_RS31080at 2354.445 kb on - strandat 2354.764 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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2,352,170 + RR42_RS31070 0.83 -0.4
2,352,170 + RR42_RS31070 0.83 +0.3
2,352,170 + RR42_RS31070 0.83 -0.0
2,352,170 + RR42_RS31070 0.83 -1.4
2,352,170 + RR42_RS31070 0.83 -0.7
2,352,170 + RR42_RS31070 0.83 +1.3
2,352,170 + RR42_RS31070 0.83 -0.3
2,352,171 + RR42_RS31070 0.83 +0.2
2,352,171 - RR42_RS31070 0.83 -0.0
2,352,171 - RR42_RS31070 0.83 -0.2
2,352,302 + +0.4
2,352,303 - -1.6
2,352,303 - -0.7
2,352,360 - -1.0
2,352,375 + +0.5
2,352,376 - +0.6
2,352,417 - -0.3
2,352,417 - -0.8
2,352,551 - +0.0
2,352,685 - -1.3
2,352,759 + -0.2
2,352,760 - -1.8
2,352,916 - RR42_RS31075 0.13 -0.6
2,353,098 + RR42_RS31075 0.30 +0.6
2,353,098 + RR42_RS31075 0.30 -0.3
2,353,098 + RR42_RS31075 0.30 -0.5
2,353,099 - RR42_RS31075 0.30 -0.3
2,353,100 + RR42_RS31075 0.31 +0.0
2,353,101 - RR42_RS31075 0.31 -0.4
2,353,149 + RR42_RS31075 0.35 -2.1
2,353,150 - RR42_RS31075 0.35 +0.0
2,353,167 + RR42_RS31075 0.37 +1.0
2,353,168 - RR42_RS31075 0.37 +0.3
2,353,392 + RR42_RS31075 0.59 -0.9
2,353,392 + RR42_RS31075 0.59 -1.6
2,353,393 - RR42_RS31075 0.59 +1.2
2,353,393 - RR42_RS31075 0.59 -0.6
2,353,476 + RR42_RS31075 0.67 +0.4
2,353,476 + RR42_RS31075 0.67 +0.0
2,353,477 - RR42_RS31075 0.67 +0.0
2,353,477 - RR42_RS31075 0.67 -0.2
2,353,480 + RR42_RS31075 0.67 -0.6
2,353,480 + RR42_RS31075 0.67 -0.8
2,353,481 - RR42_RS31075 0.68 +0.6
2,353,812 + -0.1
2,353,812 + -0.6
2,353,812 + +0.7
2,353,812 + +0.0
2,353,917 + +0.6
2,353,927 - +1.2
2,354,001 + -2.0
2,354,001 + -0.9
2,354,001 + -0.1
2,354,030 + +0.0
2,354,031 - +1.6
2,354,031 - -0.7
2,354,031 - +1.2
2,354,031 - -0.3
2,354,256 - RR42_RS31080 0.53 +0.0
2,354,256 - RR42_RS31080 0.53 +0.8
2,354,390 + RR42_RS31080 0.82 -0.6
2,354,445 - +1.4
2,354,764 + -0.7

Or see this region's nucleotide sequence